Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.30.2 (
endonuclease
)
18,621
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Purified Anabaena variabilis chromosomal DNA was partially digested with restriction
endonuclease
Sau3A and ligated into the BamHI site of plasmid pBR322. Escherichia coli 342-167, a mutant with a decreased level of
phosphoenolpyruvate carboxylase
(
PEPCase
) activity was transformed with plasmids from the A. variabilis genomic library. A transformant that grew on minimal media in the absence of glutamate was isolated and its plasmid, pTRH1, was shown to encode the A. variabilis
PEPCase
. E. coli HB101 cells transformed with plasmid pTRH1 have approx. 50 times the normal amount of
PEPCase
activity and also overproduce a protein with the apparent Mr (99,000) of the A. variabilis
PEPCase
.
...
PMID:Molecular cloning of the phosphoenolpyruvate carboxylase gene of Anabaena variabilis. 309 19
