Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.30.2 (endonuclease)
 18,621 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Nosocomial infections caused by methicillin-resistant Staphylococcus aureus (MRSA) have become an important problem with increasing frequency. In order to learn if plasmid pattern analysis can be used in epidemiologic investigations of MRSA infections, the authors did plasmid extractions of 70 MRSA stock isolates using a rapid lysostaphin lysis method. All isolates carried at least one plasmid. Most of the isolates had one large plasmid of 24-28 megadaltons (Md). Many also carried one or two small plasmids. Accordingly, 12 different patterns were identified. From these background results, we applied this method to the investigation of two small nosocomial outbreaks of MRSA infection. It was found that the analysis of plasmid pattern and restriction endonuclease analysis are more discriminative than antibiograms. Strains with the same antibiograms can be different by plasmid analysis. It is concluded that the plasmid pattern with a restriction endonuclease analysis study is a reliable method for epidemiologic study of MRSA infections.
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PMID:Epidemiologic investigation of nosocomial outbreak of methicillin-resistant Staphylococcus aureus by plasmid pattern analysis. 136 72

This study evaluated the Promega Magic Minipreps (MM) (Promega Corporation, Madison, WI) DNA purification system for use in plasmid analysis of common nosocomial bacterial pathogens. The MM system is a kit that includes lysis solutions and buffers and incorporates a minicolumn of DNA binding resin for recovery of plasmid DNA. The MM system was used according to the manufacturer's directions to recover plasmids for agarose gel electrophoresis from clinical isolates of Acinetobacter calcoaceticus, Enterobacter cloacae, and Klebsiella pneumoniae. For Salmonella enteritidis and Staphylococcus aureus, lysozyme and lysostaphin, respectively, were used for pretreatment. Plasmid DNA from ten isolates could be recovered in approximately one hour with very little manipulation and no phenol/chloroform extractions and was suitable for restriction endonuclease digestion. Compared with a standard miniprep protocol, the MM system was much easier to perform and resulted in significant cost savings due to a 50% reduction in technologist time. The authors conclude that the MM system is a convenient and cost-effective method for clinical microbiology laboratories for recovering plasmid DNA from nosocomial bacterial pathogens.
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PMID:Evaluation of a commercial DNA purification system for plasmid analysis of nosocomial bacterial pathogens. 837 41