Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.30.2 (
endonuclease
)
18,621
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Distribution of
ceruloplasmin
-coding sequences among fragments of rat nuclear DNA obtained after complete cleavage with seven restriction endonucleases was studied using highly specific complementary DNA probes. Three different procedures were used for the synthesis of cDNAs, whose relative advantages and disadvantages are discussed in this work. The number of restriction fragments carrying
ceruloplasmin
gene sequences varied from two to five depending on the enzyme used. The total molecular weight of these fragments was several times higher than the minimal length of
ceruloplasmin
structural gene deduced from the molecular size of mRNA. The restriction
endonuclease
cleavage of the partial double-stranded transcript of ceruloplasmin mRNA coding for about 60% of its length from the 3'-end has shown that distribution of restriction
endonuclease
cleavage sites on the dsDNA differs in the position of cleavage sites on the
ceruloplasmin
gene in cellular DNA. Hybridization of cDNA with total cellular DNA allows to determine 1.3-1.4 copies of
ceruloplasmin
gene in the rat haploid genome. Proceeding from the data obtained it may be stated that the rat
ceruloplasmin
gene is constructed of several structural gene segments cut by introns.
...
PMID:[Structural organization of rat ceruloplasmin gene]. 626 65
