Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.30.2 (
endonuclease
)
18,621
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
NAD(+)-dependent
15-hydroxyprostaglandin dehydrogenase
(
15-PGDH
) is a key enzyme involved in the catabolism of the prostaglandins. The cDNA for human placental
15-PGDH
has been expressed in Escherichia coli as a catalytically active protein. The polymerase chain reaction was used to introduce restriction
endonuclease
sites at each end of the
15-PGDH
coding sequence. The
15-PGDH
DNA was then inserted into the bacterial expression plasmids pUC-18 and pUC-19 which contain the isopropyl-l-thio-beta-D-galactopyranoside (IPTG) inducible lacZ promoter. Extracts from E. coli containing these expression plasmids exhibited
15-PGDH
activity which was inducible with (IPTG). Crude extracts from E. coli expressing
15-PGDH
activity were found to contain proteins of the predicted sizes in stained SDS-polyacrylamide gels and in Western blots using human placental
15-PGDH
antiserum. The specific activity in E. coli extracts was several hundred-fold higher than that seen in extracts from human placenta.
...
PMID:Expression of the cDNA for NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase as a catalytically active enzyme in Escherichia coli. 150 55
