Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.30.2 (endonuclease)
 18,621 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A total of 913 boned pork loins were selected in a commercial cutting operation on the basis of color measured by a Colormet surface colorimeter. Color (L, a, b values) at three locations, free water, and ultimate pH were measured on each loin. In addition, the genotype with respect to malignant hyperthermia was determined by a restriction endonuclease assay. Six hundred ninety-three loins were determined to be of normal (NN) genotype, 198 heterozygote (Nn), and 22 homozygote (nn). This distribution reflected the selection strategy and not the incidence in the general population. Meat was darker (P < .01) and free water was reduced (P < .01) in NN loins compared to Nn or nn loins. Ultimate pH was lower (P < .01) in Nn loins than in NN loins. An increase in the incidence of Nn or nn meat samples was observed as the paleness of the loins or their free water content increased, or as the ultimate pH of the meat decreased. Based on this study, approximately 30% of meat classified as PSE on a color basis would come from heterozygote pigs.
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PMID:Effect of the genotype for malignant hyperthermia as determined by a restriction endonuclease assay on the quality characteristics of commercial pork loins. 844 Jun 62

Sclerosing hemangioma of the lung remains poorly understood, and it is still unclear whether this lesion is neoplastic or not. It consists of two major cell types, pale cells and cuboidal cells. We analyzed the clonality of each cell types from six female cases of surgically resected sclerosing hemangioma. The pale cells and cuboidal cells were separated by microdissection from methanol-fixed sections, and DNA was extracted for clonal analysis based on an X-chromosome-linked polymorphic marker, the human androgen receptor (HUMARA) gene or the phosphoglycerate kinase (PGK) gene. The HUMARA and PGK genes were found to be amplified with or without digestion by the methylation-sensitive restrictive endonuclease HpaII. Five of six cases were informative. Pale cells and cuboidal cells showed the same monoclonality in all of the informative cases, whereas the control cells showed a polyclonal pattern. Our results demonstrated that sclerosing hemangioma is caused by monoclonal expansion of cells, confirming that it is a neoplasia. Moreover, the present data indicate that both pale cells and cuboidal cells are derived from the same cell.
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PMID:Monoclonality of both pale cells and cuboidal cells of sclerosing hemangioma of the lung. 954 67

Postweaning multisystemic wasting syndrome (PMWS), a new disease in Hungary, was recognized in a swine herd located in Southeast Hungary, during the early winter of 1999. The first clinical signs of paleness, anaemia, and leanness appeared immediately after weaning, at the age of 40-50 days. Pustules were frequently observed on the skin of the trunk, and signs of necrotic dermatitis were also visible. A syndrome of poor growth and wasting was characteristic of the affected pigs. A porcine circovirus (PCV), the suspected causative agent, was detected by polymerase chain reaction (PCR). Sequencing data and restriction endonuclease (RE) analysis of the PCR products suggested that the virus belonged to the PCV-II group where all the causative agents of PMWS are also grouped.
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PMID:New pig disease in Hungary: postweaning multisystemic wasting syndrome caused by circovirus (short communication). 1140 63