Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.30.2 (endonuclease)
 18,621 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

pMHC25, a recombinant plasmid containing myosin heavy chain (MHC) cDNA sequences from differentiated myotubes of the L6E9 rat cell line, has been shown to hybridize to all sarcomeric MHC mRNAs so far tested but not to nonsarcomeric MHC mRNAs. In addition, pMHC25 hybridizes to multiple restriction endonuclease-digested fragments of rat genomic DNA corresponding to different MHC genomic sequences. Thus, the MHC gene represented by pMHC25 is a member of a sarcomeric MHC multigene family that has regions of sequence homology shared among its members. This sarcomeric MHC multigene family has been estimated to be composed of a minimum of seven genes, some of which are polymorphic in the rat. We have also determined that pMHC25 hybridizes to MHC gene sequences in genomic DNA of all species that have striated muscle, ranging from nematodes to man. Sarcomeric MHC genes, therefore, have been horizontally and vertically conserved in evolution. Additionally, we have used the pMHC25 plasmid to demonstrate that MHC genes do not undergo rearrangement or amplification during muscle cell differentiation.
 ...
PMID:Sarcomeric myosin heavy chain is coded by a highly conserved multigene family. 629 Oct 33

Cloned myosin heavy chain DNA probes from rat and human were hybridized to restriction endonuclease digests of genomic DNA from somatic cell hybrids and their parental cells. The mouse myosin heavy chain genes detectable by this assay were located on chromosome 11, and three different human sarcomeric myosin heavy chain genes were mapped to the short arm of chromosome 17. A synteny between myosin heavy chain and two unrelated markers, thymidine kinase and galactokinase, was found to be preserved in the rodent and human genomes.
 ...
PMID:Multigene family for sarcomeric myosin heavy chain in mouse and human DNA: localization on a single chromosome. 687 74

We have isolated and characterised the 5' region of a member of the carp myosin heavy chain gene family. Expression of this gene has previously been shown to be induced by an increase in environmental temperature and is restricted to the small-diameter white myotomal muscle fibres which are associated with growth. The whole isoform gene, including potential regulatory sequence 5' to the transcription start site and the 3' untranslated region was cloned in a lambda2001 bacteriophage vector. Studies of the structure of the 5'-end of the gene revealed high amino acid sequence similarity with translated exons 3-7 of mammalian myosin heavy chain genes indicating identical exon/intron boundaries. The overall length of the gene was however only about one half of that in mammals and birds due to shorter introns. The region 5' to the transcription unit was sequenced and revealed the presence of putative TATA and CCAAAT boxes. In order to study the regulation of expression, a series of endonuclease-generated fragments from the 5' flanking sequence were spliced to chloramphenicol acetyltransferase reporter vectors and used in cell transfection assays or direct gene injection into carp skeletal muscle. The 5' flanking region, which contains a consensus sequence known as an E-box (CANNTG) and a MEF2 binding site, was shown to improve the expression of the reporter gene in fish acclimated at 18 degrees C or 28 degrees C. Unlike the coding region, there was little similarity between the 5'-upstream sequence (promoter region) when compared with sequences flanking the 5'-end of the other myosin heavy chain genes in mammals or chicken.
 ...
PMID:The characterisation of the 5' regulatory region of a temperature-induced myosin-heavy-chain gene associated with myotomal muscle growth in the carp. 866 10

In order to evaluate the genotype of CAST gene and its relationship with the muscle histology and other postmortem traits. 158 Jinpi F2 pigs were electrically stunned and exsanguinated. The blood and muscle samples were collected, and both postmortem and meat traits were recorded. PCR-RFLP, PAS and myosin heavy chain immunohistochemistry were employed to explore the relationship between the genotypes and the muscle histology. Based on the PAS reactivity, the muscle fibers can be divided into three types: I, II-a and II-b. Myosin heavy chain immunohistochemistry could differentiated the fibers into slow fibers and fast fibers, the ratio of slow fibers and fast fibers is about 7.62% and 92.38% respectively. The amplification products of the region between 6th and 7th exon of CAST gene were digested by HinfI and discriminated 2 polymorphic sites (524bp and 632bp) in endonuclease map. Only 3 genotypes (AA,AB and BB) was distinguished out. The frequency of AA, AB and BB is 0.1795, 0.5897 and 0.2308 respectively. The frequency of A and B is 0.4743 and 0.5256 respectively. Different genotypes had no statistical significant effect on area, water holding capacity, pH value, and conductivity of longissimus dorsi muscle. The results revealed that the genotypes had a significant effect on the aspect ratio of muscle and showed a negative correlation with the percentage of intramuscular connective tissue.
 ...
PMID:[The histological characteristics of longissimus dorsi and its correlation with restriction fragments polymorphism of calpastatin gene in f2 Jinghua x Pietrain crossbred pigs]. 1825 33