Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.30.2 (endonuclease)
 18,621 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Fifty-five strains received as Haemophilus vaginalis or as catalase-negative coryneform bacteria from the vagina together with 61 marker cultures were subjected to numerical phenetic analyses using 149 unit characters. The data were examined using the simple matching (SSM), Jaccard (SJ) and pattern (DP) coefficients and clustering was achieved using the average linkage algorithm. Cluster composition was not markedly affected by the coefficient used or by test error, estimated at 6 . 5%. The H. vaginalis strains formed a tight cluster which was only distantly related to representatives of the genera arthrobacter, Cellulomonas, Corynebacterium sensu stricto, Erysipelothrix, Haemophilus, Kurthia, Lactobacillus, Listeria and Propionibacterium but shared a high overall affinity to unclassified catalase-negative coryneforms which formed a discrete taxon, cluster 9. The H. vaginalis strains could be distinguished from the related strains in cluster 9 by several unrelated phenotypic characters. Using the S1 endonuclease assay, DNA-DNA hybridizations were performed with representative strains from the numerical as well as with reference strains of Bifidobacterium and Actinomyces. Haemophilus vaginalis was found to be a genotypically legitimate group and its DNA showed little homology with DNA from the marker strains tested. The DNA base composition of H. vaginalis was 42 to 44 mol % guanine plus cytosine. A new genus should be created to incorporate strains known as H. vaginalis or Corynebacterium vaginale. The name Gardnerella vaginalis proposed by Greenwood & Pickett (1979) is supported.
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PMID:A taxonomic study of Gardnerella vaginalis (Haemophilus vaginalis) Gardner and Dukes 1955. 697 16

Restriction fragment length polymorphisms of the 16S rRNA genes of Erysipelothrix strains were studied by cleavage of the chromosomal DNA with restriction endonuclease EcoRI, followed by hybridization to a 420-bp internal fragment of the 16S rRNA gene. Thirty-two Erysipelothrix type and reference strains were classified, together with seven field strains. Reference strains of all serotypes and the type strains of Erysipelothrix rhusiopathiae and Erysipelothrix tonsillarum were included. Nine ribopatterns were observed. Pattern A was represented by 16 strains and included strains of serotypes 1b, 2 to 8, 11 to 13, 15 to 17, 19, and 23. Pattern B was represented by two strains (serotypes 1a and 9). Pattern C was represented by five strains (serotypes 5, 6, and 21). Pattern D was represented by one strain of serotype 4. Pattern E was represented by 11 strains of serotypes 2, 7, 10, 20, 22, 24, and 25. Patterns F, G, H, and I were each represented by a single strain of serotypes 26, 2, 18, and 3, respectively. All the different ribopatterns had some bands in common. Patterns B, C, and D were most similar to pattern A, while patterns F, G, H, and I resembled pattern E. Partial sequencing of the 16S rRNA gene of nine selected strains resulted in three different sequences, i.e., the typical E. rhusiopathiae sequence, the E. tonsillarum sequence, and a third sequence found for two strains. Strains of the same serotype were found to have different ribopatterns as well as different partial 16S rDNA sequences.
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PMID:Classification of Erysipelothrix strains on the basis of restriction fragment length polymorphisms. 753 73