Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.30.2 (
endonuclease
)
18,621
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The long non-coding RNA
ANRIL
, antisense to the
CDKN2B
locus, is transcribed from a gene that encompasses multiple disease-associated polymorphisms. Despite the identification of multiple isoforms of
ANRIL
, expression of certain transcripts has been found to be tissue-specific and the characterisation of
ANRIL
transcripts remains incomplete. Several functions have been associated with
ANRIL
.
In our judgement, studies on
ANRIL
functionality are premature pending a more complete appreciation of the profusion of isoforms. We found differential expression of
ANRIL
exons, which indicates that multiple isoforms exist in melanoma cells. In addition to linear isoforms, we identified circular forms of
ANRIL
(
circANRIL
). Further characterisation of
circANR
IL
in two patient-derived
metastatic melanoma
cell lines (NZM7 and NZM37) revealed the existence of a rich assortment of circular isoforms. Moreover, in the two melanoma cell lines investigated, the complements of
circANRIL
isoforms were almost completely different. Novel exons were also discovered. We also found the family of linear
ANRIL
was enriched in the nucleus, whilst the circular isoforms were enriched in the cytoplasm and they differed markedly in stability. With respect to the variable processing of
circANRIL
species, bioinformatic analysis indicated that intronic
Arthrobacter luteus
(Alu) restriction
endonuclease
inverted repeats and exon skipping were not involved in selection of back-spliced exon junctions. Based on our findings, we hypothesise that "
ANRIL
" has wholly distinct dual sets of functions in melanoma. This reveals the dynamic nature of the locus and constitutes a basis for investigating the functions of
ANRIL
in melanoma.
...
PMID:Multiple Isoforms of ANRIL in Melanoma Cells: Structural Complexity Suggests Variations in Processing. 2972 53
