Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.30.2 (
endonuclease
)
18,621
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
It is difficult to make a precise diagnosis for
intestinal tuberculosis
and differentiate it from Crohn's disease. For evaluating the efficacy of PCR assay in these two aspects, 36 specimens of
intestinal tuberculosis
and 26 of Crohn's disease from surgical resections and endoscopic biopsies were subjected to PCR assay. Oligonucleotides derived from IS 6110 sequence, which is a repeated in M. tuberculosis chromosome and highly specific for the M. tuberculosis complex, were used as a primer. The specificity of PCR products was confirmed by digestion with Sal I restrictive
endonuclease
and southern blot hybridization by using digoxigenin-labeled probe. The results showed that M. tuberculosis DNA was identified in 27 of the 36 specimens of
intestinal tuberculosis
, but none in those of 26 Crohn's disease. In conclusion, PCR is a rapid, sensitive and specific method for the diagnosis of
intestinal tuberculosis
, and it is also valuable in differentiating
intestinal tuberculosis
from Crohn's disease.
...
PMID:[The value of polymerase chain reaction assay in the diagnosis of intestinal tuberculosis and differentiation from Crohn's disease]. 760 Aug 74
It is difficult to make a precise diagnosis of
intestinal tuberculosis
and to differentiate it from Crohn's disease. For evaluating Polymerase Chain Reaction (PCR) assay in these two aspects, 36 specimens of
intestinal tuberculosis
from surgical resections and endoscopic biopsies and 26 Crohn's disease samples were subjected to PCR assay. 21 specimens of normal colon tissue surrounding cancer were used as the control. Oligonucleotides derived from the IS 6110 sequence, which is repeated in M. tuberculosis chromosome and highly specific for the M. tuberculosis complex, were used as a primer. The amplified PCR products were detected by examination of ethidium-bromide-stained polyacrylamide gels. The specificity of PCR products was confirmed by digestion with Sal I restrictive
endonuclease
and southern blot hybridization using digoxigenin-labeled probe. The results showed that the M. tuberculosis DNA was identified in 27/36
intestinal tuberculosis
, but none of 26 Crohn's disease. Acid fast bacilli were only found in 16/36
intestinal tuberculosis
. In conclusion, as a rapid, sensitive, and specific pathogenic method in diagnosis of
intestinal tuberculosis
, PCR assay has been developed in this study, and is considered valuable in the differentiation between
intestinal tuberculosis
and Crohn's disease.
...
PMID:Value of polymerase chain reaction assay in diagnosis of intestinal tuberculosis and differentiation from Crohn's disease. 779 30
