Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.30.2 (endonuclease)
 18,621 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

An accurate, sensitive, and quick (approximately 3 h) method for determining the sex of ovine embryos was developed using polymerase chain reaction (PCR) primers derived from an ovine-specific Y-chromosome random amplified polymorphic DNA marker (UcdO43). The accuracy and sensitivity of the assay were first tested using genomic DNA from 10 males and 10 females of five different sheep breeds, and then tested using serial dilutions of male-in-female DNA. The assay was 100% accurate in confirming the sex of the individuals and the ovine male-specific fragment was detected in dilutions containing as little as 10 pg of male DNA in 50 ng of female DNA. The assay was also confirmed to be specific for the ovine Y-chromosome as bovine, caprine, porcine, murine, and human DNA did not amplify. The ovine embryo sexing method is a duplex PCR system that also includes ZFY/ZFX primers. ZFY/ZFX provide an internal positive control for amplification as well as a means to confirm the results obtained with the UcdO43 primers. All embryo sexing results (36/36) from our method were in agreement with the ZFY/ZFX assay results. However, while our method requires an internal control to detect PCR failure, it has the advantages of not requiring nested PCR or restriction endonuclease digestion of the PCR product, and concerns about cross-species contamination are eliminated.
 ...
PMID:Ovine-specific Y-chromosome RAPD-SCAR marker for embryo sexing. 917 11

The polymerase chain reaction (PCR) has been used to amplify a region of the ZFX and ZFY genes from DNA in human blood and other tissues, for determination of the sex of an individual. In the present study DNA was extracted from the pulp of 21 male and 24 female fresh human third molar teeth. A region of the ZFX and ZFY genes was amplified by PCR and analysed by digestion of the amplified DNA with HaeIII restriction endonuclease. The digested PCR products were run on a 2 per cent agarose gel. Males were distinguished from females by having a fragment of 317 base pairs which was absent in females. Identification of the sex of the individual was 100 per cent accurate in each case. A blind study of random samples of the same teeth, used to assess the reproducibility of the technique evoked an identical result. This method provides an accurate alternative to skeletal measurements and histological staining techniques for the sexing of individuals from small amounts of DNA.
 ...
PMID:A simple method for the determination of sex from the pulp of freshly extracted human teeth utilizing the polymerase chain reaction. 958 6