Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.30.2 (endonuclease)
 18,621 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Leptospira, a member of the order Spirochaetales, is the causative agent of leptospirosis, an important zoonosis encountered worldwide. The Leptospira interrogans serovar Sejroe was grown in EMJH medium and its DNA was isolated using standard techniques. The LipL32 gene was amplified using the reported primer of Kirschneri of LipL32. The amplified product was found to comprise 756 base pairs. This amplified gene fragment of LipL32 lipoprotein was cloned in E. coli (DH5 alpha) cells using pDrive plasmid as a vector. The recombinant cells were selected on LB agar medium containing ampicillin, X-gal and isopropyl-beta-D-thiogalactopyranoside. Plasmid was extracted from the recombinant white colonies, and restriction endonuclease (RE) analysis was carried out using PstI and SalI. On partial sequence analysis, the product exhibited 756 base pairs, corresponding to 251 amino acids. The cloned gene could be further used for expression of recombinant protein for serodiagnosis of leptospirosis.
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PMID:Cloning and sequence analysis of the gene encoding LipL32 of Leptospira interrogans serovar Sejroe. 1722 65

Giardia duodenalis is one of the most common and important protozoan parasites of the gastrointestinal tract in humans and animals, especially in developing countries. The purpose of this study was determining prevalence of Giardia genotypes specially zoonosis genotypes in sheep and goat in eastern of iran slaughterers.This cross-sectional study was conducted during April to November 2019. 300 fecal samples were collected from the rectum of sheep and goats. The samples were subjected to DNA extraction after sucrose gradient purification. A fragment of the glutamate dehydrogenase gene (gdh) was amplified by semi-nested PCR and genotype diagnosis was performed by digestion of the secondary PCR product with restriction enzymes RsaI and Nla IV. The prevalence of Giardia was found as (274/300) by the molecular method. Restriction endonuclease digestion of the nested-PCR product showed; among 274 positive isolates, 95 were typed as assemblage E, 15 as assemblage B, 87 assemblage AI, 45 assemblage AII, and 32 assemblege C. In this study, frequency of different assemblages of G. duodenalis was determined in sheep and goats by gdh gene and PCR-RFLP method. Same of other studies, assemblage E was dominant genotype in sheep and goats. Isolation of zoonotic assemblages as AI, AII, and BIII showed that sheep and goats should be considered as a source for human infection.
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PMID:Frequency of different genotypes of Giardia duodenalis in slaughtered sheep and goat in east of iran. 3280 15