Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.30.2 (endonuclease)
 18,621 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Two new human papovavirus isolates (JMV and MMV) from the urines of patients with Wiskott-Aldrich syndrome were morphologically and serologically identical to BK virus (BKV). The genomes of these two new isolates were found to be indistinguishable from prototype BKV DNA in a variety of nucleic acid hybridization experiments. Like BKV DNA, JMV and MMV DNAs share approximately 20% of their polynucleotide sequences with simian virus 40 DNA. The genome of JMV was indistinguishable from that of BKV by restriction endonuclease analysis; MMV DNA contained three instead of four R-Hind cleavage sites and one rather than no R-HpaII cleavage sites. Physical maps of the BKV and MMV genomes were constructed using restriction endonucleases, and these maps were oriented to the map of simian virus 40 DNA.
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PMID:Physical map of the BK virus genome. 17 Apr 25

Human fibroblast cell lines established from skin biopsies of patients with Wiskott-Aldrich syndrome (WAS), a sex-linked immunodeficiency disorder, were found to have unusually high sensitivity to SV40 infection. When examined by immunofluorescence, two of the cell lines showed almost 100% positive staining for tumor and viral antigens 4 days after infection, while the third cell line showed 75% positive cells for both antigens. Marked cytopathic changes were seen in infected cultures and viral yields of 10(8) pfu/ml were obtained. After six serial passages in WAS cells, the viral DNA was examined by restriction endonuclease analysis and found to have HindIII cleavage pattern similar to that of DNA from SV40 grown in monkey cells.
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PMID:High-titer SV40 replication in human fibroblast cell lines derived from patients with Wiskott-Aldrich syndrome. 631 84