Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.30.2 (
endonuclease
)
18,621
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
DNA polymerase lambda (
Pol lambda
) is a novel enzyme of the family X of DNA polymerases.
Pol lambda
has some properties in common with DNA polymerase beta (Pol beta). The substrate properties of
Pol lambda
were compared to Pol beta using DNAs mimicking short-patch (SP) and long-patch (LP) base excision repair (BER) intermediates as well as recessed template primers. In the present work, the influence of several BER proteins such as flap-
endonuclease
-1 (FEN1), PCNA, and apurinic/apyrimidinic endonuclease-1 (APE1) on the activity of
Pol lambda
was investigated.
Pol lambda
is unable to catalyze strand displacement synthesis using nicked DNA, although this enzyme efficiently incorporates a dNMP into a one-nucleotide gap. FEN1 and PCNA stimulate the strand displacement activity of
Pol lambda
. FEN1 processes nicked DNA, thus removing a barrier to
Pol lambda
DNA synthesis. It results in a one-nucleotide gapped DNA molecule that is a favorite substrate of
Pol lambda
. Photocrosslinking and functional assay show that
Pol lambda
is less efficient than Pol beta in binding to nicked DNA. APE1 has no influence on the strand displacement activity of
Pol lambda
though it stimulates strand displacement synthesis catalyzed with Pol beta. It is suggested that
Pol lambda
plays a role in the SP BER rather than contributes to the LP BER pathway.
...
PMID:Comparison of functional properties of mammalian DNA polymerase lambda and DNA polymerase beta in reactions of DNA synthesis related to DNA repair. 1597 54
