Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.30.1 (
S1 nuclease
)
3,660
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The murine
Mac-2
gene is composed of six exons dispersed over 10.5 kilobases.
S1 nuclease
mapping showed multiple transcription initiation sites, clustered within a 30-base pair region. Sequence analysis revealed that a consensus initiator sequence is located in this area which lacks a TATA motif. The untranslated first exon contains an alternative splice donor site, confirming the existence of two cDNA species with the potential to encode proteins differing at their NH2 termini. In vitro expression and translocation experiments demonstrate that both of the alternatively spliced variants of
Mac-2
encode proteins which lack a functional signal peptide. Subcellular fractionation studies indicate that most of the
Mac-2
protein is present in the cytosol. These results support the view that
Mac-2
is exported from the cell by an unusual mechanism which does not depend on the presence of a signal peptide.
...
PMID:Structure of the murine Mac-2 gene. Splice variants encode proteins lacking functional signal peptides. 850 79
