Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.30.1 (S1 nuclease)
 3,660 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In developing rodent nervous system, although the appearance of Thy-1 mRNA, as seen by in situ hybridisation, is in general quickly followed by the appearance of immunohistochemically detectable protein, there are certain sites where a delay of several days occurs between expression of detectable message and protein. Mouse Purkinje cells exemplify this behaviour and are the dominant Thy-1-expressing cell in early postnatal cerebellum, so allowing quantitative, homogenate-based methods to be used to test whether such a lag in protein expression does occur. Measurement of Thy-1 mRNA (by slot blot) and protein (by radioimmunoassay) shows a substantial excess of Thy-1 message, compared to protein accumulating in the tissue, during the first postnatal week, which is not found in tissues (rat cerebellum, and rat or mouse cerebrum) where no lag is apparent in appearance of Thy-1 protein from the section-based methods. The species of Thy-1 mRNA produced by Purkinje cells does not appear to change during development, as assessed either in terms of its size (by northern blotting) or in the heterogeneous pattern of transcription initiation sites used (assessed by S1 nuclease protection analysis). Appearance of Thy-1 protein in these cells, therefore, seems to be regulated posttranscriptionally.
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PMID:Expression of the neuronal surface glycoprotein Thy-1 does not follow appearance of its mRNA in developing mouse Purkinje cells. 134 38

Recombinant bacteriophage and cosmid clones containing the gene for the mouse Thy-1.2 glycoprotein were isolated and characterized. The complete sequence of the gene was determined, including a previously unidentified exon located 2.1 kb upstream of the portion of the gene encoding the Thy-1.2 glycoprotein. The transcriptional initiation site was located by S1 nuclease protection mapping in both T lymphocytes and neural cells and was found to be located immediately upstream of this exon. S1 nuclease protection mapping was also used to define the 3' end of the Thy-1.2 transcription unit, and no evidence for alternate mRNA processing was found. Thus, the mouse Thy-1.2 gene is 5447 base pairs in length, including promoter sequences, rather than 2094 as previously described. The mouse and rat Thy-1 genes are highly homologous in both introns and exons. However, the mouse Thy-1 cDNA and rat Thy-1 cDNA differ significantly in sequence in the 5' untranslated region. This suggests that the transcriptional initiation site of the mouse and rat genes may be located at different positions within the genomic sequence and may be related to the differing tissue distribution of Thy-1 in the two species.
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PMID:The mouse Thy-1.2 glycoprotein gene: complete sequence and identification of an unusual promoter. 286 59