Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.30.1 (S1 nuclease)
 3,660 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The major neuronal populations of the primate cerebral cortex can be classified immunocytochemically according to their transmitters and in terms of the differential expression of certain other molecules such as neuropeptides, calcium-binding proteins and protein kinases. We have been able to chart the time course of developmental expression of these molecules and to show that gene expression for many of them is regulated in adult and infant animals by afferent activity entering the cortex. In the visual cortex of adult monkeys, levels of immunocytochemically detectable gamma aminobutyric acid (GABA), of its synthesizing enzyme glutamic acid decarboxylase (GAD) and of the tachykinins are greatly reduced in deprived ocular dominance columns within 24 h of blocking impulse activity in the optic nerve by intraocular injection of tetrodotoxin (TTX). Conversely, levels of immunocytochemically detectable calcium-calmodulin-dependent protein kinase (CAMII kinase) are increased in deprived eye dominance columns. These effects are quickly reversible on restoration of binocular vision, and experiments involving in situ hybridization and S1 nuclease protection assays show that the changes are associated with parallel changes in mRNA levels for preprotachykinin and CAM II kinase, but not for GAD, which appears to be regulated by post-transcriptional mechanisms. Experiments in the primate somatic sensory cortex suggest comparable activity-dependent effects on gene expression there also. It is proposed that effects of this type underlie the establishment of cortical maps during development and their activity-dependent mutability in adulthood.
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PMID:The role of afferent activity in the maintenance of primate neocorticalfunction. 217 67

In Pseudomonas putida carrying the CAM plasmid, the operon (camDCAB) encoding enzymes involved in the degradation pathway of D-camphor is negatively regulated by the CamR protein, and camR is autorepressed. S1 nuclease mapping revealed that camDCAB and camR were divergently transcribed from overlapping promoters, the transcription start sites were separated by 11 bp, and transcriptions of the cam operon (camDCAB) and camR increased about 10- and 4-fold, respectively, immediately after addition of camphor. The transcriptions of camDCAB and camR were negatively regulated through the interaction of the CamR protein with the one operator located in the overlapping promoter region. In vitro transcription experiments were performed to characterize the regulation of cam genes. The camR promoter was initiated by P. putida RNA polymerase containing sigma 70, but transcription from the camDCAB promoter by sigma 70 holoenzyme was not observed. The purified CamR protein repressed in vitro transcription from the camR promoter. This repression was suppressed by camphor. The RNA polymerase binding region of the camR promoter was identified by using DNase I footprinting. In addition, footprinting studies revealed that the CamR protein and RNA polymerase coexisted on the promoter region in a joint nonproductive complex.
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PMID:Transcription of the cam operon and camR genes in Pseudomonas putida PpG1. 769 53