Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.30.1 (
S1 nuclease
)
3,660
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Glucose-6-phosphate dehydrogenase
(
G6PD
) activity differs among tissues and, in liver, with the dietary state of the mouse. Tissue-specific differences in
G6PD
activity in adipose tissue, liver, kidney, and heart were associated with similar differences in the amount of
G6PD
mRNA. Regulation of mRNA amount by dietary fat was only observed in liver. In mice fed a low-fat diet, the relative amounts of
G6PD
mRNA were 3:1:1:0.38, respectively, in the four tissues. Further, the amount of precursor mRNA for
G6PD
in liver, kidney, and heart reflected the amount of mature mRNA in these tissues, suggesting differing transcriptional activity. Our
S1 nuclease
and primer-extension analyses indicated that the same transcriptional start site is used in liver, kidney, and adipose tissue, resulting in a common 5' end of the mRNA in these tissues. Thus, differential regulation is not attributable to alternate promoter usage. A DNase hypersensitivity analysis of the 5' end of the
G6PD
gene identified three hypersensitive sites (HS): HS 1 and HS 2 were present in all tissues, whereas HS 3 was liver specific. Thus, regulation of
G6PD
expression involves both dietary and tissue-specific signals that appear to act via different mechanisms.
...
PMID:Structural characterization and tissue-specific expression of the mouse glucose-6-phosphate dehydrogenase gene. 953 8
