Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.30.1 (S1 nuclease)
 3,660 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

S1 nuclease hydrolysis and hydroxyapatite chromatography were used to study the effect of the alkylating antibiotic, streptozotocin, on the secondary structure of DNA. Native calf thymus DNA was alkylated in vitro with increasing concentrations of streptozotocin and subjected to S1 nuclease hydrolysis. An increasing degree of DNA degradation was seen, suggesting a destabilization of the secondary structure. Indirect evidence, deduced from alkaline hydrolysis, effect of NaCl on S1 nuclease hydrolysis, and hydroxyapatite chromatographic analysis of alkylated DNA, suggested a significant alkylation of DNA phosphates in addition to DNA bases. Nicotinamide has been reported to alter the cytotoxic and carcinogenic effects of streptozotocin. Our experiments indicate that in the presence of nicotinamide, streptozotocin causes the formation of a greater proportion of alkylated bases in relation to alkyl phosphotriesters. This may have significance in relation to the differential cytotoxicity of streptozotocin in the absence and presence of nicotinamide.
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PMID:Effect of alkylation with streptozotocin on the secondary structure of DNA. 302 Dec 52

We have isolated from a BALB/c genomic library a 17.5-kilobase (kb) DNA fragment containing the entire mouse sn-glycerol-3-phosphate dehydrogenase (glycerol-P dehydrogenase) gene, and a substantial portion of the flanking regions. The DNA sequence of 10.8 kb of this fragment was determined. Using this information, together with the DNA sequence of several partial glycerol-P dehydrogenase cDNA clones and the rabbit glycerol-P dehydrogenase amino acid sequence, we determined the structural organization of the mouse glycerol-P dehydrogenase gene. The gene is 7.3 kb long and contains 8 exons. Transcription starts 19 base pairs 5' of the ATG initiation codon. Sequence analysis and S1 nuclease mapping indicated that the 8th exon contains coding sequences for the last 31 amino acids of glycerol-P-dehydrogenase, followed by 1.7 kb of 3' untranslated mRNA sequence. The mouse glycerol-P dehydrogenase amino acid sequence determined from the DNA sequence is 90% homologous with the rabbit enzyme. An examination of the exon/intron organization of the mouse glycerol-P dehydrogenase gene shows that intron 3 precisely separates the NAD-binding and the catalytic domains and intron 2 separates the adenine- and nicotinamide-binding regions.
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PMID:Primary structure of the mouse glycerol-3-phosphate dehydrogenase gene. 375 21