Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.30.1 (S1 nuclease)
 3,660 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We report the entire primary structure of the human alpha 3(IV) collagen chain determined from cDNA clones and polymerase chain reaction-amplified DNAs. The deduced amino acid sequence demonstrates that the complete translation product consists of 1670 amino acid residues and the mature alpha 3(IV) chain contains 1642 residues with a corresponding calculated molecular mass of 161,753. The full-length translated polypeptide has a signal peptide of 28 amino acids, a 1410-residue collagenous domain starting with a 14-residue noncollagenous sequence, and a 232-residue NC1 domain. There are 23 noncollagenous interruptions in the Gly-X-Y repeat sequence of the collagenous domain. The major transcription start site of the alpha 3(IV) chain gene was also determined from genomic DNA by primer extension and S1 nuclease protection assays. Northern analysis revealed coexpression of the alpha 3(IV) and alpha 4(IV) chains in tissues where expression was observed such as in kidney, muscle, and lung.
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PMID:Complete primary structure of the human alpha 3(IV) collagen chain. Coexpression of the alpha 3(IV) and alpha 4(IV) collagen chains in human tissues. 808 1

The levels of abscisic acid (ABA) during embryogenesis in the soybean (Glycine max) cultivar Dare were quantitated. An increase in the quantity of ABA per cotyledon was correlated with a decrease in the chlorophyll a/b binding (Cab) protein gene mRNA population. Soybean cotyledons were cultured in vitro in the presence or absence of ABA. Quantitation of cotyledonary ABA levels and Cab mRNA levels indicated that the application of 5 x 10(-5) molar and 5 x 10(-6) molar exogenous ABA decreased Cab mRNA prevalences. S1 nuclease protection experiments demonstrated that exogenous ABA modulated the level of Cab3 mRNA. These data strongly suggest that one of the developmental regulators of Cab gene expression during soybean embryogeny is the plant hormone, ABA; ABA negatively regulates Cab mRNA accumulation.
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PMID:Abscisic Acid Negatively Regulates Expression of Chlorophyll a/b Binding Protein Genes during Soybean Embryogeny. 1666 19

Changes in the temporal and spatial distribution of the mRNAs for the chlorophyll-a/b-binding-protein gene (Cab) in cotyledons from developing soybean (Glycine max (L.) Merr.) seedlings were studied. Cab mRNAs could be detected in the polysomal polyadenylated poly(A)(+) mRNA population of cotyledons within 3 d after start of imbibition, prior to their emergence from soil, and declined prior to the onset of cotyledonary senescence. The Cab mRNA levels were compared to the levels of rbcS (ribulose-1,5-bisphosphate carboxylase small subunit) mRNAs in cotyledons and distinct differences in their expression programs were noted. Quantitative analyses with S1 nuclease were used to monitor the accumulation of the mRNAs of individual members of the Cab gene family. Cab 3, Cab 4, and Cab 5 mRNAs were differentially regulated in the cotyledons during post-germinative development. Cab 4 was the most abundant Cab gene mRNA representing approx. 4.3% of the cotyledonary polysomal poly(A)(+) mRNA population. In-situ hybridizations using methacrylate-imbedded tissue and (3)H-antisense- and -sense-strand RNA probes were used to determine the qualitative and quantitative distribution of Cab RNAs in cotyledonary cells. Cab RNAs were most abundant in the palisade cells. These results indicate an interaction of both developmental and environmental cues in modulating the expression of the Cab gene family in soybean cotyledons.
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PMID:Spatial and temporal expression of Cab mRNAs in cotyledons of the developing soybean seedling. 2418 66


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