Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.30.1 (S1 nuclease)
 3,660 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The class I gene family of the rabbit consists of 8 to 12 members and includes a gene that is transcribed only in T cells and in lymphoid tissues containing T cells. A cDNA clone, pR27, was isolated from a cDNA library constructed using mRNA from the T cell line RL-5. The pR27 insert was 1.9 kb long and included sequences that correspond to class I exons 2, 3, and 4 encoding external domains. Intron 4 sequence was found downstream followed by an unusually long 3' region which contained a sequence highly repeated in the rabbit genome. Although the pR27 exons encode structures typical of class I antigens, structural comparisons show no close relationship to other rabbit class I genes in the 3' regions, and no relationship to the 3' region of any murine gene including those encoded in the T1a region. By contrast, similarity of the 3' sequence has been found with human genes of the HLA-A type both by sequence comparison and by DNA hybridization studies. Southern blot analyses with a specific probe from this clone indicated that the gene encoding pR27 is present as a single copy in all rabbit DNA samples examined. Northern blot analysis and S1 nuclease mapping studies revealed that transcripts corresponding to pR27 are present in virally transformed T cell lines, thymus, and, to a lesser extent, in spleen and appendix but not in nonlymphoid tissue. The sizes of the detected transcripts (2.8 and 3.9 kb) were larger than that normally observed for a class I gene.
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PMID:A rabbit class I major histocompatibility complex gene with a T cell-specific expression pattern. 349 86

In order to determine the relative quantities of different HLA-A and -B mRNAs in cells, we have developed a simple and reliable method by using reverse transcription-polymerase chain reaction (RT-PCR), denaturing gradient gel electrophoresis (DGGE) and phosphor imaging analysis. Cytoplasmic RNA from lymphoblastoid cell lines with well-characterized HLA phenotypes are reversely transcribed with a primer specific for all HLA-A and -B antigens. The first-strand cDNA is used as template for quantitative PCR. The primer pair used for quantitative PCR are specific for all class I HLA and one of the primers is labeled with [gamma-32P]ATP. The amplified sequences include parts of exon 2 and exon 3 which contain most polymorphic residues in class I HLA molecules. The RT-PCR products containing the amplified HLA-A and -B sequences are separated by DGGE. The radioactivities of different DNA bands separated in denaturing gradient polyacrylamide gels are measured by phosphor imaging and used to determine the relative amounts of HLA-A and -B mRNAs. This approach is validated by using samples containing known quantities of different HLA-A and -B mRNA transcripts and confirmed by S1 nuclease protection assay. The combined RT-PCR/DGGE approach therefore provides a simple and reliable method for quantitation of relative amounts of different HLA-A and -B mRNAs in cells. This method should also be useful for studying the expression of other highly conserved and duplicated genes.
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PMID:Measurement of relative quantities of different HLA-A and -B mRNAs in cells by reverse transcription-polymerase chain reaction and denaturing gradient gel electrophoresis. 913 31