Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.30.1 (
S1 nuclease
)
3,660
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The cyclin-dependent kinase inhibitor
p27Kip1
plays an important role in regulating cell-cycle progression.
p27Kip1
directly inhibits the catalytic activity of cyclin/cdks (cyclin-dependent kinase) complexes and/or interferes physically with cyclin/cdks activation by CAK. Interestingly, the expression level of
p27Kip1
mRNA was maximal in resting Go T-cells and rapidly declined following anti-CD3 activation. We report here the cloning of
p27Kip1
gene from murine genomic DNA and the functional analysis of the promoter of the
p27Kip1
gene. The gene consists of at least three exons and spans more than 5.6 kb of DNA. Primer extension and
nuclease S1
protection analysis revealed two major transcription initiation sites. The promoter region lacked a TATA box but contained potential binding sites for the transcriptional factors including two Sp1, CRE, Myb and NFkB located at positions -153, -178, -286, -875, and -1011, respectively. To analyze the regulatory mechanisms controlling
p27Kip1
gene expression, we characterized the 5'-flanking region from nt -1609 to +178. The -326 to -615 region contained positive regulatory elements.
...
PMID:Characterization of the murine cyclin-dependent kinase inhibitor gene p27Kip1. 897 54
