Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.30.1 (
S1 nuclease
)
3,660
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
In this work, a new kind of peroxidase-mimicking DNAzyme (G-quadruplex-hemin DNAzyme, G4-hemin) was constructed by using hemin-modified G-rich DNA (hemin-G-DNA). Experimental results demonstrated that the G-rich DNA can form a G-quadruplex structure by the inducement of terminally modified hemin, rendering the assembly of hemin and G-quadruplex structure spontaneously and efficiently. As a result, G-hemin revealed higher peroxidase activity than traditional G-quadruplex/hemin DNAzyme (G4/hemin). Besides, different from G4/hemin, G4-hemin was constructed in one step without the participation of metal ions and adscititious hemin. Accordingly, the construction procedure was significantly simplified and the background signal from dissociative hemin was remarkably reduced. In a proof-of-concept trial, according to the colorimetric signals of G4-hemin, a novel biosensor for the detection of
S1 nuclease
activity was established, which provides a novel perspective for designing peroxidase-mimicking DNAzyme-based biosensors.
ACS
Appl Mater Interfaces 2016 Jan 13
PMID:Construction of Metal-Ion-Free G-quadruplex-Hemin DNAzyme and Its Application in S1 Nuclease Detection. 2666 85
The recent discovery of reversible chemical modifications on mRNA has opened a new era of post-transcriptional gene regulation in eukaryotes. Among the 15 types of modifications identified in mRNA of eukaryotes, N7-methylguanosine (m
7
G) is unique owing to its presence in the 5' cap structure. It remains unknown whether m
7
G is also present internally in mRNA, and this is largely attributed to the lack of an appropriate analytical method to differentiate internal m
7
G in mRNA from that in the 5' cap. To address this analytical challenge, we developed a novel strategy of combining differential enzymatic digestion with liquid chromatography-tandem mass spectrometry analysis to quantify the levels of these two types of m
7
G modifications in mRNA. In particular, we found that
S1 nuclease
and phosphodiesterase I exhibit differential activities toward internal and 5'-terminal m
7
G. By using this method, we found that internal m
7
G was present in mRNA of cultured human cells as well as plants and rat tissue. In addition, our results showed that plants contain higher levels of internal m
7
G in mRNA than mammals. We also observed that exposure of rice to cadmium (Cd) stimulated marked diminution in the levels of m
7
G at both the 5' cap and internal positions of mRNA, which was correlated with the Cd-induced elevated expression of m
7
G-decapping enzymes. Taken together, we reported here a strategy to distinguish internal and 5'-terminal m
7
G in mRNA, and by using this method, we demonstrated the prevalence of internal m
7
G modification in mRNA, which we believe will stimulate future functional studies of m
7
G on post-transcriptional gene regulation in eukaryotes.
ACS
Chem Biol 2018 12 21
PMID:Existence of Internal N7-Methylguanosine Modification in mRNA Determined by Differential Enzyme Treatment Coupled with Mass Spectrometry Analysis. 2931 62
Herein, we report a carbazole (Cz) ligand that displays distinct turn-on fluorescence signals upon interaction with human telomeric G-quadruplex ( h-TELO) and nuclease enzymes. Interestingly, Cz selectively binds and stabilizes the mixed hybrid topology of h-TELO G-quadruplex that withstands digestion by exonucleases and
nuclease S1
. The distinct fluorescence signatures of Cz-stabilized h-TELO with nucleases are used to design conceptually novel DNA devices for selectively detecting the enzymatic activity of DNase I as well as performing logic operations. An INHIBIT logic gate is constructed using h-TELO and DNase I as the inputs while the inputs of h-TELO and
nuclease S1
form a YES logic gate. Furthermore, a two-input two-output reusable logic device with "multireset" function is developed by using h-TELO and DNase I as inputs. On the basis of this platform, combinatorial logic systems (INHIBIT-INHIBIT and NOR-OR) have been successfully installed using different combinations of nucleases as inputs. Moreover, this new strategy of using a synthetic dual emissive probe and enzyme/DNA inputs for constructing reusable logic device may find important applications in biological computing and information processing.
ACS
Synth Biol 2018 05 18
PMID:Enzyme-Regulated DNA-Based Logic Device. 2966 71
Bacterial determination, emerging as a critical step in the understanding of increasingly serious bacterial contaminations, remains a major challenge. Herein, a novel chemiluminescence biosensor was exploited for the ultrasensitive determination of nuclease activity and bacteria, in which, hemin, the chemiluminescent (CL) tag molecule was encapsulated into ordered mesopores of mesoporous silica nanoparticles with a specific DNA gate. The capped DNA could be specifically switched upon exposure to the DNA nuclease or bacterial lysate and allowed for an increased release of the encapsulated hemin, which therefore resulted in an obviously enhanced CL signal for the luminol-H
2
O
2
system. Attributed to this unique behavior with the linear or sigmoidal relationship between CL intensity and DNA nuclease or bacterial concentration, the as-prepared CL biosensor could detect
S1 nuclease
activity in the concentration range 0.01-10.0 U with a detection limit of 0.1 mU, and
Escherichia coli
O157:H7 (
E. coli
) or
Staphylococcus aureus
(
S. aureus
) in the concentration ranges 10
1
to 10
9
cfu mL
-1
. The detection limit of
E. coli
and
S. aureus
was calculated to be 3.0 and 2.5 cfu mL
-1
, respectively, which was comparable or even better than that of previous studies. Thus, this detection method could achieve detectable levels without cell enrichment overnight. Moreover, the proposed biosensing system could be conducted in the homogeneous solution without separation and washing, greatly improving the reaction efficiency and simplifying the procedure. As expected, the novel CL biosensor promised a great potential for simple and convenient detection of nuclease and bacteria in fields such as food bacterial contamination, pharmaceuticals, and clinical analysis.
ACS
Sens 2019 11 22
PMID:Ultrasensitive Chemiluminescence Biosensor for Nuclease and Bacterial Determination Based on Hemin-Encapsulated Mesoporous Silica Nanoparticles. 3167 71
