Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.30.1 (S1 nuclease)
 3,660 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The synthesis of several nucleic acid block polymers of the general type dGn.rCidCk is described. The key steps in this procedure were the joining of dCk oligomers, protected at the 3'-OH with an acetyl group, to rCi oligomers by T4 DNA ligase and the purification of the products by RPC-5 column chromatography. The block polymers were characterized by 20% polyacrylamide gel electrophoresis, UV and CD spectra, analytical Cs2SO4 buoyant density analyses, helix-coil transitions and S1 nuclease studies. NMR studies on one member of this series, dGn.rC11dC16, were reported recently (Selsing, E., Wells, R.D., Early, T.A., and Kearns, D.R. (1978) Nature 275, 249-250). The NMR studies and the results described herein indicate that these block polymers are linear duplexes with two adjoining conformations yet are hydrogen-bonded and base-stacked throughout with minimal disruption of the helix at the junction of the two conformations. Computer model building studies described in the following paper (Selsing, E., Wells, R.D., Alden, C.J., and Arnott, S. (1979) J. Biol. Chem. 254, 5417-5422) predict that these nucleic acids contain a bend at the junction region.
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PMID:Polynucleotide block polymers consisting of a DNA.RNA hybrid joined to a DNA.DNA duplex. Synthesis and characterization of dGn.rCidCk duplexes. 44 59

We have investigated the sites sensitive to the S1 nuclease which are presented in a supercoiled plasmid, pSVS 3.3. This plasmid contains the entire transcription unit plus 5'- and 3'-flanking regions of the rat seminal vesicle IV gene on a 3.3 kb Eco RI fragment (Harris et al., 1983). Using a cDNA probe of rat seminal vesicle secretion product IV (SVS IV) mRNA, we have demonstrated S1 nuclease-sensitive sites in the 5'-flanking and in the 3'-flanking regions of SVS IV gene. The site in the 5'flanking region has been mapped to approximately 117 bp upstream from the CAP site. This site at -117 bp can potentially form a cruciform structure as inferred from the presence of inverted repeats in this region (Lilley, 1981; Panayotatos and Wells, 1981). We have now shown such a structure most probably exists. The S1 nuclease-sensitive site in the 3'flanking region is present in an area of highly repeated sequence.
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PMID:The seminal vesicle secretion IV gene: detection of S1 nuclease-sensitive sites in supercoiled plasmid pSVS 3.3. 630 19