Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.3.9 (glucose-6-phosphatase)
 3,081 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

1. Homogenates of bovine splenic nerves were subjected to differential and sucrose density gradient centrifugation. From the low-speed supernatant a high-speed sediment (mitochondria, lysosomes, microsomes and noradrenaline (NA) vesicles) was obtained. By density gradient centrifugation of this sediment it was shown that NA vesicles are slightly less dense than mitochondria, but denser than microsomes.2. In further experiments a mitochondrial and a microsomal sediment were obtained. The mitochondrial sediment was fractionated with a short centrifugation time over a density gradient ranging from 0.6 to 1.2 M sucrose. Mitochondria (fumarase and succinate-dehydrogenase) and lysosomes (acid ribonuclease and deoxyribonuclease) sedimented to the bottom of the tube. The highest concentration of NA vesicles was found in a medium position. There was only a small amount of microsomes (glucose-6-phosphatase) present.3. The microsomal sediment was centrifuged for 150 min over a density gradient ranging from 0.8 to 1.4 M sucrose. The microsomes remained on the top of the gradient. There were also some mitochondria and lysosomes present. The NA vesicles were found in highest concentration in the middle of the gradient (at about 1.2 M sucrose).4. With the use of these two density gradients, the subcellular distribution of dopamine-beta-hydroxylase, monoamine oxidase and ATPase was studied. Dopamine-beta-hydroxylase was found to be localized in the NA vesicles. Monoamine oxidase was mainly recovered in mitochondria; a small part of the enzyme appeared to be microsomal. ATPase was present in microsomal elements.
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PMID:Bovine splenic nerve: characterization of noradrenaline-containing vesicles and other cell organelles by density gradient centrifugation. 431 May 9

Experiments were run on three groups of healthy guinea pigs. One group was given ethionamide, kanamycin and PASA another was given ethionamide, kanamycin and pyrazinamide while the third served as a control. These studies permitted to establish that the above drugs affect the activity of enzymes involved in the metabolism of carbohydrates and nucleic acids. Activity of glucose-6-phosphatase and aldolase significantly decreased in liver, brain and lung tissue. At the same time, activity of deoxyribonuclease and ribonuclease in the tissues concerned sharply increased. Changes in activity of phosphohexose isomerase, lactate dehydrogenase and aminotransferases in these tissues was statistically insignificant.
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PMID:[Effect of various combinations of antibacterial drugs on enzyme activity in guinea pig tissues]. 531 14