Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.3.9 (
glucose-6-phosphatase
)
3,081
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The objective of our study was to determine the cellular localisation of
glucose-6-phosphatase
in developing human kidney using monospecific antiserum and a standard immunohistochemical method (peroxidase-antiperoxidase,
PAP
) on formalin fixed and paraffin embedded tissue. In embryonic and early fetal development of the metanephric kidney,
glucose-6-phosphatase
is located primarily in derivatives of the ureteric bud such as the pelvis, calyces and collecting ducts. In mid-fetal life as nephrons evolve and develop they become increasingly immunoreactive to
glucose-6-phosphatase
, such that in mature metanephric kidney the proximal tubules are highly reactive for
glucose-6-phosphatase
with other elements of the nephron also immunopositive albeit at lower reactivities. In addition the parietal layer of Bowman's capsule and some cells of the visceral layer are immunopositive. Only with the development of nephrons does the early predominance of
glucose-6-phosphatase
immunoreactivity to ureteric bud derivatives change: in mature kidney the reactivity in the collecting ducts is a small proportion of the total. In proximal tubular cells the distribution of
glucose-6-phosphatase
immunoreactivity is relatively uniform throughout development in contrast to collecting ducts where in fetal life this reactivity is displaced to the apices and basal areas by intracellular glycogen deposits. The mesonephric kidney has a similar pattern of
glucose-6-phosphatase
immunoreactivity to that of metanephric kidney. The availability of monospecific antiserum to
glucose-6-phosphatase
and immunohistochemical methods now allows an alternative approach to cellular localisation. Many of the difficulties in the fixation of tissue and assay of
glucose-6-phosphatase
activity inherent in conventional histochemical methods are avoided by such methods.
...
PMID:Immunohistochemical localisation of glucose-6-phosphatase in developing human kidney. 796 Sep 40
