Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.3.9 (glucose-6-phosphatase)
 3,081 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The in vitro effects of vitamin D3 metabolites, parathyroid extract (PTE), purified parathyroid hormone (bPTH), vitamin A, and heparin on acid and alkaline phosphatases in rat or mouse calvaria in culture were investigated. Results show that: (a) when compared to values found in half calvaria incubated for 24 h in control medium, the bone acid and alkaline phosphatase content is significantly higher in paired halves incubated with PTE (L USP/ml), bPTH (4 x 10(-8)M), heparin (5 USP/ml), vitamin A (23 USP/ml), 25-(OH)D3 (2.5 x 10(-11) to 2.5 x 10(-8)M), 24,25-(OH)2D3, and 1,25-(OH)2D3 (2.5 x 10(-12) to 2.5 x 10(-7M); (b) the presence of 24,25-(OH)2D3 at low concentrations in the incubation medium decreases significantly the PTE, bPTH, vitamin A, or heparin induced stimulation of the phosphatase activities. This interaction is also observed when measuring beta glucuronidase and glucose-6-phosphatase activities and 45Ca release from previously labeled mouse calvaria; (c) a similar activity could not be found with 1,25-(OH)2D3 suggesting that 24,25-(OH)2D3 may have a specific role in bone metabolism.
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PMID:Interaction of 24,25-dihydroxyvitamin D3 and parathyroid hormone on bone enzymes in vitro. 11 87

In order to determine the relationship of parathyroid hormone and levels of dietary protein and calcium with the activity of renal glucose-6-phosphatase (G6Pase), effects of two levels of dietary protein, namely, 25 and 75%, on the enzyme activity were compared at three levels of dietary calcium, namely, 0.06, 0.63, and 1.83%, with the use of intact and thyroparathyroidectomized (TPTX) rats. In intact rats, 0.06% dietary calcium caused an increase in renal G6Pase activity in rats fed the high carbohydrate diet, and dietary calcium in excess (1.83%) caused the enzyme activity to decrease. Similar responses in the activity of renal G6Pase to the variation of dietary calcium levels were seen in rats fed the high protein diet, but significant differences were not obtained. In TPTX rats fed the high carbohydrate diet, the activity of renal G6Pase was significantly decreased compared with that of intact rats. When TPTX rats were fed the high protein diet, however, no significant decrease in the enzyme activity was observed. Free access to aqueous 0.1% CaC1(2) solution by TPTX rats tended to restore the activity of renal G6Pase and serum calcium concentrations depressed by thyroparathyroidectomy. In addition, a significant correlation was observed between the total activity of renal G6Pase and serum calcium concentrations. Hypothyroidism produced by oral administration of propylthiouracil (0.05% of diets) did not affect the enzyme activity in the kidneys of rats fed the high carbohydrate and the high protein diets. The results suggest that the activity of renal G6Pase of rats fed the high protein diet might be less susceptible both to dietary calcium levels and to parathyroid function than that of rats fed the high carbohydrate diet.
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PMID:Effects of dietary protein and calcium levels on renal glucose-6-phosphatase activity of intact and thyroparathyroidectomized rats. 16 33