Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.3.8 (
phytase
)
1,997
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Phytase (EC 3.1.3.26) and phytic acid (myo-inositol hexaphosphate) play an important environmental role in poultry industry and have a health aspect in food industry. Novel biosensors have been developed for simple, one step quantitative phytic acid and
phytase
detection. A system based on the sequentially acting enzyme
phytase
and pyruvate oxidase (POD) was employed for the development of
phytase
and phytic acid biosensors.
Poly
(carbamoylsulphonate) (PCS) hydrogel immobilized POD electrode was applied for the detection of
phytase
. It was based on the indication of phosphate ions produced by the hydrolysis of phytic acid. The
phytase
biosensor showed a linear response ranging from 0.5 to 6.0 units/ml. A bi-enzyme sensor based on co-immobilization of
phytase
and POD was developed for the detection of phytic acid on the basis of amperometric detection of the enzymatically-generated hydrogen peroxide at 0.6 V versus Ag/AgCl. It showed a linear response ranging from 0.2 to 2.0 mM with a detection limit of 0.002 mM.
...
PMID:Novel biosensors for quantitative phytic acid and phytase measurement. 1501 58