Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound   Target Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound

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Query: EC:3.1.3.8 (phytase)
1,997 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

1. Three experiments were carried out to determine the phosphorus (P) requirements of laying hens aged 34 to 58 weeks (experiment 1), 59 to 70 weeks (experiment 2) and 22 to 50 weeks (experiment 3) given diets containing wheat, sorghum and soyabean meals as the main ingredients. Dietary total P (Pt) varied between 3.2 and 7.3 g/kg (experiment 1), 3.2 and 4.6 g/kg (experiment 2) and 3.0 and 6.6 g/kg (experiment 3). Hens were housed at either 18 degrees or 30 degrees C (experiments 1 and 2) and uncontrolled temperature (experiment 3), and in experiment 2 diets were fed without or with a phytase supplement of 500 units/g. 2. Dietary Pt had no significant effect on production measures in any experiment. Increases in dietary Pt adversely influenced egg shell quality although uterine calcium (Ca), ATPase and carbonic anhydrase activities were unaffected. 3. A 3-d-feeding trial in experiment 1 gave maximum Pt retentions of 228 mg/d at 18 degrees C and 204 mg/d at 30 degrees C. These were obtained with diets containing, respectively, 4.6 and 6.0 g Pt/kg. 4. Plasma inorganic P (Pi) increased consistently with increases in dietary Pt at all temperatures but plasma total Ca, and tibia Ca and P, were unaffected. 5. The inclusion of the phytase supplement in diets containing 3.2 and 4.6 g Pt/kg had an adverse effect on egg production at both temperatures in experiment 2. 6. A dietary Pt concentration of 3.2 g/kg, providing a calculated 1.2 g available P (Pav)/kg, with a dietary phytase activity of less than 200 units/kg, satisfied the P requirements of the hens used in these studies. However, the data from experiment 3 suggest that the Pt requirement of some flocks fed on wheat-based diets may be lower than 3.2 g/kg.
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PMID:Phosphorus requirements of laying hens fed on wheat-based diets. 765 2

Seven experiments were conducted to determine the efficacy of 1,25-dihydroxycholecalciferol [1,25-(OH)2D3] and microbial phytase in improving P, Zn and Mn utilization of chicks fed P, Zn- and Mn-deficient soy protein diets containing surfeit levels of cholecalciferol. Efficacy of 1 alpha-hydroxycholecalciferol (1 alpha-OH D3) was also studied. A dose titration study indicated that supplemental phytase at 1200 units/kg diet would increase bone ash by at least 65% when added to a corn-soybean meal diet containing 0.43 g P/100 g (0.1 g nonphytate P/100 g). These responses were similar to those obtained from supplemental P (0.1 g/100 g) as KH2PO4 or from added 1,25-(OH)2D3 (10 micrograms/kg). Dietary addition of both 1200 units phytase and 10 micrograms/kg 1,25-(OH)2D3 elicited bone ash responses that were near 100%. When chicks were fed a Zn-deficient soy-concentrate diet (13 mg Zn/kg), diet supplementation with 1,25-(OH)2D3 or phytase increased growth rate by 40% and tibia Zn content by > 100%; adding 1,25-(OH)2D3 together with phytase increased tibia Zn content by 160%. Utilization of both Zn and Mn contained in the corn-soybean meal diet also was markedly enhanced by supplemental phytase, 1,25-(OH)2D3, or the combination. The cholecalciferol analog 1 alpha-OH D3 was found to improve dietary P utilization maximally (70% bone ash response) at a dose of 20 micrograms/kg diet, and effects were additive when 1 alpha-OH D3 was fed in the presence of phytase.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:1 alpha-Hydroxylated cholecalciferol compounds act additively with microbial phytase to improve phosphorus, zinc and manganese utilization in chicks fed soy-based diets. 766 60

A high phytic acid diet (barley, wheat, soya bean meal, 4 g P/kg, of that 2/3 phytate P) without added phytase, with phytase supplement (1000 U/kg diet) or with supplementary phosphate (2,2g P/kg diet) was examined with 3 x 12 weaned piglets. The high dietary P level due to phosphate addition, significantly improved body weight gain and feed:gain ratio. The phytase effect on these criteria was small. The phytase but more the supplementary phosphate increased P concentration of serum. In case of phytase addition pigs had 10% less ash P and Ca in the rib than animals of phosphate group. In the group without phytase or P addition the ash, P and Ca concentration of bone were decreased by 20%.
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PMID:[Evaluation of Aspergillus niger phytase and dietary phosphate in weaned piglets. 1. Growth, blood serum and bone status]. 766 74

A high phytic acid diet (barley, wheat, soya bean meal, 4 g P/kg diet, of that 2/3 phytate P) without added phytase, with phytase supplement (1000 U/kg diet) or with supplementary phosphate (2.2 g P/kg diet) was examined with 3 x 12 weaned piglets. The three diets contained 8 g Ca/kg. At the end of experiment 6 pigs/group were slaughtered. In animal body (as empty body) the content and gain of ash, P, Ca, protein and fat were detected. P supplementation and supplementary phytase had no effect on dry matter, protein and fat content of animal body. The enzyme but more the supplementary phosphate increased mineralization of skeleton and made the animal body higher in ash, P and Ca content. Piglets without supplementary phytase and P gained 1.1 g P daily. Phytase increased daily P gain by 0.5 g (P < 0.05), the phosphate by 1.4 g (P < 0.001). The daily Ca gain was 1.7; 2.8 and 5.1 g in the different groups. A piglet (body weight 20 kg) with sufficient P and Ca in the diet gains 5 g P and 10 g Ca per kg body weight gain (empty body).
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PMID:[Evaluation of Aspergillus niger phytase and phosphate in weaned piglets. 2. Content and gain of fat, energy, ash, Ca and P in the animal body]. 766 82

The use of canola meal, an abundant side-product of canola oil processing in Canada, as animal feed is hampered by high phytic acid levels that reduce metal cation availability. Aspergillus carbonarius grows well in a solid canola meal medium, produces phytase and reduces the phytic acid content to zero. Inorganic phosphate addition at a concentration of 1 mg and 5 mg/110 g solid-state culture system results in better growth of the microorganism, higher rates and levels of phytase production, and faster reduction of phytic acid content. Phosphate concentrations of 50 mg and 100 mg/110 g inoculated system had a negative effect affecting primarily the initial rates of biomass and phytase production and phytic acid content reduction. Models that predict biomass production (expressed as glucosamine content) and phytase, as well as the reduction of phytic acid content in the solid-state cultures supplemented with phosphate are reported. They fit the experimental results reasonably well (with a maximum deviation of 7%).
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PMID:The effect of phosphate concentration on phytase production and the reduction of phytic acid content in canola meal by Aspergillus carbonarius during a solid-state fermentation process. 776 33

The presence of phytase activities in condensed cane molasses solubles (CCMS) and CCMS-Lactobacillus (Lacto) were determined. Single Comb White Leghorn layers were fed .25 and .45% available P (AP) diets supplemented with CCMS and CCMS-Lacto for nine 28-d periods to determine phytase activities of the gastrointestinal (GI) tract contents and intestine, liver, and pancreatic tissues, the GI tract pH, the P and Ca retention, and layer performance. Six dietary treatments were corn-soybean (C-S) control, C-S+CCMS, and C-S+CCMS-1,100 mg Lacto/kg diet (ppm) [4.4 x 10(7) cfu/mg Lacto] each with .25 and .45% AP. The CCMS were used as a carrier for the Lacto, and the CCMS and CCMS-Lacto premix were incorporated at 2% of the diets. Phytase activity was much higher in CCMS-Lacto premix than in CCMS. Phytase activities of the crop contents were higher with the CCMS-Lacto diets regardless of the AP level. Intestinal phytase activity was higher with the .45% AP CCMS-Lacto diet than the unsupplemented .45% AP diets. Lactobacillus supplementation did not stimulate phytase activities in the intestinal contents or liver and pancreatic tissues. The pH of the crop and intestinal contents were much lower for the Lacto-fed layers than the layers fed unsupplemented diets regardless of dietary AP levels. No differences in Ca retentions were observed with Lacto supplementation regardless of the dietary AP levels. However, higher P retentions were observed with the Lacto supplementation in the .25% AP diet. Layers fed .25 and .45% AP Lacto-supplemented diets had lower hen-day egg production, poorer feed conversion value, consumed slightly more feed, produced less egg mass, and laid larger eggs than the layers fed .25 and .45% AP unsupplemented diets. Lacto supplementation to .25% AP diet produced eggs with higher specific gravity than the unsupplemented .45% AP diet, but not different from unsupplemented .25% AP diet. Layers fed the .25% AP diets had lower BW gains then layers fed the .45% AP diets regardless of lacto supplementation. Phytase activity was present in the lacto source, and the presence of phytase and Lacto supplementation to a .25% AP diet improved P retention in layers.
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PMID:Phytase activity, phosphorus and calcium retention, and performance of single comb White Leghorn layers fed diets containing two levels of available phosphorus and supplemented with direct-fed microbials. 781 30

Phytases are the primary enzymes responsible for the hydrolysis of phytic acid, myo-inositol-1,2,3,4,5,6-hexakisphosphate (I-1,2,3,4,5,6-P6). A number of phytases with varying specificities, properties, and localizations hydrolyze phytic acid present in cells. The specificity of hydrolysis of phytic acid by alkaline phytase from lily (Lilium longiflorum L.) pollen is described. Structures of the intermediate inositol phosphates and the final product were established by a variety of nuclear magnetic resonance techniques (1H-, 31P-, and 31P-1H-detected multiple quantum coherence spectroscopy, and total correlation spectroscopy). On the basis of the structures identified we have proposed a scheme of hydrolysis of phytic acid. Initial hydrolysis of the phosphate ester occurs at the D-5 position of phytic acid to yield the symmetrical I-1,2,3,4,6-P5. The two subsequent dephosphorylations occur adjacent to the D-5 hydroxyl group to yield I-1,2,3-P3 as the final product. Alkaline phytase differs from other phytases in the specificity of hydrolysis of phosphate esters on the inositol ring, its high substrate specificity for phytic acid, and biochemical properties such as susceptibility to activation by calcium and inhibition by fluoride. The physiological significance of alkaline phytase and the biological role of I-1,2,3-P3 remain to be identified.
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PMID:Specificity of hydrolysis of phytic acid by alkaline phytase from lily pollen. 784 60

Bile Cu accumulation in Cu-depleted chicks fed Cu concentrations between .56 and 1.56 mg Cu/kg (0, .5, or 1 mg supplemental Cu/kg) was used to investigate the effect of microbial phytase at 600 U/kg and 1,25-dihydroxycholecalciferol [1,25-(OH)2D3] at 10 micrograms/kg on Cu bioavailability from dehulled soybean meal (SBM) and cottonseed meal (CSM). The bioavailability of Cu (relative to CuSO4.5H2O, which was set at 100%) in SBM and CSM was 43 and 39%, respectively. Phytase addition (600 U/kg diet) decreased Cu bioavailability in SBM to 21%, but did not affect that in CSM (34%). Copper bioavailability in SBM was not affected by addition of 1,25-(OH)2D3 (10 micrograms/kg diet), but that in CSM was nearly doubled by 1,25-(OH)2D3 supplementation.
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PMID:Effect of microbial phytase and 1,25-dihydroxycholecalciferol on dietary copper utilization in chicks. 789 99

An Aspergillus niger (ficuum) genomic DNA lambda EMBL3 library was probed with a 354-bp DNA fragment obtained by polymerase chain reaction of A. niger DNA with oligonucleotides based on partial amino acid sequence of a pH 2.5 optimum acid phosphatase. A clone containing a 1605 bp segment (phyB) encoding the 479 amino acid enzyme was isolated and found to contain four exons. Global alignment revealed 23.5% homology to Aspergillus niger phytase (PhyA); four regions of extensive homology were identified. Some of these regions may contain catalytic sites for phosphatase function.
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PMID:Identification and cloning of a second phytase gene (phyB) from Aspergillus niger (ficuum). 791 10

Three secreted acid phosphatases had previously been characterized from Aspergillus ficuum grown under conditions of limited phosphate. One of these could not be readily separated from AFPhyB, a pH 2.5 optimum acid phosphatase with phytase activity. From extensive protein sequence analysis and subsequent cloning of the gene, we have shown that the AFPhyB protein fraction contains a fourth secreted acid phosphatase (AFPhoA) that has 64% homology to a phosphate-repressible acid phosphatase from Penicillium chrysogenum. Garnier plot analysis revealed that the putative phosphate catalytic domain of AFPhoA at His215Asp216 is similar to those of other acid phosphatases, but that AFPhoA lacks the phosphate-binding motif RHGXRXP of known histidine phosphatases.
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PMID:An acid phosphatase from Aspergillus ficuum has homology to Penicillium chrysogenum PhoA. 794 93


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