Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.3.8 (phytase)
 1,997 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

These studies were conducted to determine if supplementation of a corn-soybean meal diet with 1,25-dihydroxycholecalciferol [1,25-(OH)2D3] would increase the utilization of natural phytate phosphorus by broiler chickens. Two experiments were conducted to evaluate the effect of dietary 1,25-(OH)2D3 in the presence and absence of supplemental phytase and at several dietary levels of inorganic phosphorus supplementation. The criteria measured in these studies were weight gain, gain:feed ratio, bone ash, rickets due to phosphorus deficiency, plasma calcium and phosphorus and retention of calcium, phosphorus and phytate phosphorus. In the first experiment, the types and amounts of fecal inositol phosphates were determined by HPLC, and the total fecal phytate was determined by the classic FeCl3 precipitation technique. In the first experiment, the addition of 1,25-(OH)2D3 to the diet in the presence of dietary phytase resulted in greater 9-d weight and bone ash and lower incidence of rickets; the retention of total fecal phytate and phytate phosphorus was greater than in controls. The second experiment was a complete 2 x 2 x 2 factorial design [phosphorus levels x phytase x 1,25-(OH)2D3]. The addition of 1,25-(OH)2D3 alone to the diet resulted in greater 9-d weight and bone ash, lower incidence of rickets, and greater retention of total calcium and phosphorus and phytate phosphorus. The highest retention of phytate phosphorus (79.4%) was obtained when both phytase and 1,25-(OH)2D3 were present in the diet. The possible mode of action and importance of these results in many areas of nutrition and environmental science are discussed.
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PMID:Dietary 1,25-dihydroxycholecalciferol supplementation increases natural phytate phosphorus utilization in chickens. 838 10

Two experiments were conducted to compare the effects of supplementation with 1,25-dihydroxycholecalciferol [1,25-(OH)2D3] and a commercial phytase on P utilization by broiler males. Experiment 1 was conducted with three levels of total dietary P (0.45,0.55, and 0.65%) in corn-soybean meal diets supplemented with 5 micrograms/kg of 1,25-(OH)2D3, 600 units/kg of phytase, or the combination of these supplements in a factorial arrangement from 0 to 21 d in battery brooders. A second experiment was conducted with a similar design except that it was carried out in floor pens for a period of 35 d. In Experiment 1, maximal BW was obtained at 0.65% P in chicks receiving the basal diet, 0.55% P in chicks receiving phytase or 1,25-(OH)2D3, and 0.45% P in chicks fed both supplements. Bone ash for chicks receiving the basal, phytase, 1,25-(OH)2D3, and combination treatments at 0.45% total dietary P were 26.6, 34.9, 35.1, and 38.8%. There were significant interactions between phytase and 1,25-(OH)2D3 for BW, bone ash, and incidence of rickets. Similar results were noticed in Experiment 2, with the exception that 1,25-(OH)2D3 had little influence on BW from 0 to 3 wk, likely due to slightly higher dietary P. From 3 to 5 wk, BW and bone ash were increased by each supplement and further increased by their combination. These interactions suggest different mechanisms of action for these supplements in influencing phytate P utilization.
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PMID:Additive effects of 1,25-dihydroxycholecalciferol and phytase on phytate phosphorus utilization and related parameters in broiler chickens. 865 Jan

Three experiments were conducted to determine the effects of supplementing 1,25-dihydroxycholecalciferol [1,25-(OH)2D3] and a commercial phytase product on Ca and P requirements of 0- to 21-d-old broiler males. These experiments were conducted with four levels of dietary Ca and P in corn-soybean diets with and without supplementation of 5 micrograms/kg of 1,25-(OH)2D3, 600 units/kg of phytase, and the combination of these supplements. The results show that these levels of phytase and 1,25-(OH)2D3 can replace up to 0.1% of the inorganic P for criteria such as BW, bone ash, and plasma P. Both supplements increased phytate P retention, whereas higher levels of Ca and P decreased phytate P retention. The addition of 1,25-(OH)2D3, but not phytase, reduced Ca requirements and decreased the incidence of tibial dyschondroplasia. The combination of these levels of phytase and 1,25-(OH)2D3 replaced 0.2% inorganic P for criteria such as BW, bone ash, and P rickets. Total dietary P requirements are estimated to be between 0.55 and 0.60% at the levels of phytase and 1,25-(OH)2D3, listed above, or 0.45% when the combination is added. The Ca requirements are estimated to be 0.77% when 1,25-(OH)2D3 is added to the diet and 0.9 to 0.95% when phytase is added.
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PMID:Effects of phytase and 1,25-dihydroxycholecalciferol on phytate utilization and the quantitative requirement for calcium and phosphorus in young broiler chickens. 865 Jan 18

This study evaluated the overall performance of 0-to-16-d-old, mixed-sex, Cobb x Cobb broiler chicks when dietary phytase levels were supplemented in excess of industry standards. The experimental diet used consisted of a basal corn-soybean meal diet that contained an analyzed 22.2% CP, 0.88% Ca, a deficient total P (tP) level of 0.46% (phytate P = 0.272%), and calculated ME of 3.123 kcal/g diet on an as-is basis. In addition to a positive control diet [0.70% tP], the dietary phytase levels evaluated were 0, 93.75, 187.5, 350, 750, 1,500, 3,000, 6,000, and 12,000 U/kg of diet. Supplementing phytase from 0 to 12,000 U significantly increased body weight gain from 287 to 515 g/chick, feed intake from 381 to 595 g/chick, gain to feed from 0.755 to 0.866, plasma P from 2.5 to 7.1 mg/100 mL, tibia ash from 26 to 41%, tibia ash weight from 0.200 to 0.601 g/tibia, tP retention from 51 to 80%, phytate P disappearance from 40% to 95%, apparent N retention from 58 to 78%, AMEn from 3,216 to 3,415 kcal/kg diet, and reduced P rickets from 80 to 3%. Using nonlinear regression analysis on log-transformed phytase levels, gain to feed, apparent N retention, and AME, responded linearly with respective R2 values of 0.76, 0.82, and 0.72, whereas body weight gain, feed intake, plasma P, P rickets, tP retention, phytate P disappearance, tibia ash percentage, and tibia ash weight responded quadratically with respective R2 values of 0.93, 0.88, 0.85, 0.84, 0.91, 0.96, 0.96, and 0.98. Few statistical differences existed between response data for broilers consuming the positive control diet or diets containing 1,500 to 12,000 U of phytase (P > 0.05). This finding indicates that broilers consuming a tP-deficient corn-soybean meal diet can achieve maximum performance when phytase is supplemented to 12,000 U/kg diet and that current phytase supplementation levels within the poultry industry may need to be reevaluated.
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PMID:Graded levels of phytase past industry standards improves broiler performance. 1271 Apr 90

Supplemental 1alpha-hydroxycholecalciferol (1alpha-OHD3) has been shown to have qualitatively similar and quantitatively additive effects to exogenous phytase. Two experiments were conducted from 0 to 35 d in floor pens to determine the additive effect of phytase and 1alpha-OHD3 when supplemented to Ca- and P-deficient diets. In both experiments, at least 4 replicates per treatment (50 chicks per replicate) were used. Corn-soybean-meal-and soybean-oil-based diets were fed and birds were raised in a house impervious to ultraviolet light. During the starter phase (ST), from 0 to 18 d, chicks were fed a 23% CP diet containing 0.60% Ca and 0.47% total P (tP). During the grower/finisher phase (GF), from 19 to 35 d, birds were fed a 19% CP diet containing 0.30% Ca and 0.37% tP. A combination of 1,000 phytase units/kg of Natuphos phytase and 5 microg/kg of 1alpha-OHD3 (P+1A) was supplemented to some of the feed during the ST and GF. Diets containing adequate Ca and P were also fed during the ST (0.90% Ca, 0.68% tP) and GF (0.80% Ca, 0.67% tP). Performance characteristics and the incidence of rickets and tibial dyschondroplasia were measured at 18 and 35 d. In experiment 1, unsupplemented chicks performed well but had considerable leg problems. Chicks fed P+1A during the ST or GF did not perform as well as birds fed P+1A throughout. Birds fed P+1A throughout performed as well birds fed the adequate diets without any indication of leg problems. In experiment 2, unsupplemented birds performed similarly to unsupplemented birds in experiment 1. However, chicks fed the supplements or the control diets did not perform as well or accumulate as much bone ash as birds in experiment 1, although the diets were formulated identically in both experiments. Diets with as little as 0.30% Ca and 0.37% tP appear to be adequate for broilers older than 18 d if supplemented with the correct amounts of phytase and 1alpha-OHD3. However, there are unknown variables that may limit the potential of broilers in terms of bone mineralization and bone pathology, even when adequate diets are fed.
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PMID:Phytase and 1alpha-hydroxycholecalciferol supplementation of broiler chickens during the starting and growing/finishing phases. 1633 32

Supplementation of some organic acids to a P-deficient diet has been shown to improve phytate P utilization. Two experiments were conducted from 0 to 16 d in battery brooders to determine the effect of various organic acids supplementation on phytate P utilization. In both experiments, birds were fed P-deficient corn and soybean meal-based diets. In experiment 1, citric acid, malic acid, fumaric acid, and EDTA were supplemented. Experiment 2 had a 2 x 2 factorial design with 2 sources of Met, 2-hydroxy-4-(methylthio) butanoic acid (HMB) and dl-Met, with or without 500 U/kg of phytase. In experiment 1, the addition of citric, malic, and fumaric acids increased percentage of bone ash, but only the effect of citric acid was significant. The addition of citric and malic acids also significantly increased the retention of P and phytate P (P<0.05). In experiment 2, the addition of phytase to the diet significantly increased 16-d BW gain, feed intake, percentage of bone ash, milligrams of bone ash, phytate P disappearance, and decreased the incidence of P-deficiency rickets. Methionine source did not affect 16-d BW gain, feed intake, feed efficiency, milligrams of bone ash, or P rickets incidence. However, the birds fed HMB had a higher percentage of bone ash and phytate P disappearance compared with the groups fed dl-Met only when phytase was added to the diets. The additions of citric acid and HMB improved phytate P utilization. However, the reason why some organic acids are effective whereas others are not is not apparent.
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PMID:The effect of several organic acids on phytate phosphorus hydrolysis in broiler chicks. 1833 89

Supplementation of 1alpha-hydroxycholecal-ciferol (1alpha-OHD(3)), phytase, and Ca levels has been shown to influence phytate P utilization. Two experiments were conducted with 1- to 16-d broilers to investigate the interactions between the effects of 1alpha-OHD(3), phytase, and Ca on phytate P utilization. In experiment 1, the birds were randomly allocated to 13 treatments with 6 levels of 1alpha-OHD(3) from 0 to 11microg/kg, with and without 500 U/kg of phytase supplemented to P-deficient diet. A positive control diet, adequate in Ca and P, was included. In experiment 2, a 3-dimensional rotatable design with 5 levels each of 1alpha-OHD(3), phytase, and Ca was implemented. Supplementation of 1alpha-OHD(3) and phytase increased phytate P utilization, as indicated by an increase in bone ash, phytate P disappearance, and BW. In experiment 1, no interaction between the 1alpha-OHD(3) and phytase effects was determined for any criteria, except for the incidence of P rickets. However, the second experiment showed that there were interactions between the 3 factors (1alpha-OHD(3), phytase, and Ca). The probabilities that observed variation in the 3-way interaction for measured criteria that were due to chance are: BW gain = 0.1364, G:F = 0.11, bone ash percentage = 0.0278, milligrams of bone ash/tibia = 0.0909, Ca rickets = 0.9394, P rickets = 0.4892, tibial dyschondroplasia = 0.5927, and phytate P disappearance = 0.1126. The equations obtained from the regression models were used to generate contour-surface plots. The percentage of bone ash data indicated that low Ca, high phytase, and high 1alpha-OHD(3) levels were needed to optimize phytate P utilization. The results of this study show that this experimental design may be utilized to identify the best levels of nutrients to add to a diet where complex 3-way interactions exist affecting several criteria. The equations may be used to optimize performance based on desired levels of performance and the costs of the inputs.
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PMID:Experimental approach to optimize phytate phosphorus utilization by broiler chickens by addition of supplements. 1959 81

Mineral deficiencies, especially of iron, zinc, and calcium, respectively, negatively affect human health and may lead to conditions such as iron deficiency anemia, rickets, osteoporosis, and diseases of the immune system. Cereal grains and legumes are of global importance in nutrition of monogastrics (humans and the respective domestic animals) and provide high amounts of several minerals, e.g., iron, zinc, and calcium. Nevertheless, their bioavailability is low. Plants contain phytates, the salts of phytic acid, chemically known as inositol-hexakisphosphate, which interact with several minerals and proteins. However, phytate may be hydrolysed by phytase. This enzyme is naturally present in plants and also widely distributed in microorganisms. Several food processing methods have been reported to enhance phytate hydrolysis, due to the activation of endogenous phytase activity or via the enzyme produced by microbes. In recent years, fermentation for food and feed improvement and preservation, respectively, has gained increasing interest as a promising method to degrade phytate and enhance mineral utilization in monogastrics. Indeed, several in vitro as well as in vivo studies confirm a positive effect on the utilization of minerals, such as P, Ca, Fe and Zn, using sourdough fermentation for baking or fermentation of legumes, mainly soybeans. This review summarizes the current knowledge regarding the potential of fermentation to enhance macro and trace element bioavailability in monogastric species.
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PMID:Fermentation of food and feed: A technology for efficient utilization of macro and trace elements in monogastrics. 2701 74