Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.3.5 (5'-nucleotidase)
 3,167 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Livers from rats fed the carcinogen 2-acetylaminofluorene (AAF) were analyzed at weekly or semiweekly intervals to correlate appearance of enzymatic markers in total liver homogenates with histochemical events accompanying formation of hyperplastic liver nodules. gamma-Glutamyltranspeptidase (gamma-GT)-positive foci appeared by day 11 and visible nodules were present by days 28-35. Specific activity of homogenate gamma-GT increased in parallel to formation of hyperplastic foci and nodules, declined and then rose again to 20-fold that of controls by day 77. Specific activity of ornithine decarboxylase increased in advance of that of gamma-GT, to a level of 8-fold above control during the period of formation of hyperplastic foci. An early response was a 2-fold rise in the specific activity of nucleoside diphosphate phosphatase during the first week of carcinogen administration. The specific activity of 5'-nucleotidase, known to increase during liver regeneration, declined as the animals aged and was not increased by the dietary AAF. The enzymatic alterations induced by AAF could not be mimicked by cell proliferation, diet stress or the hepatotoxicity induced by feeding 1.87% 4-acetamidophenol.
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PMID:Early biochemical alterations induced by 2-acetylaminofluorene in rat liver. 614 71

In one experiment Swiss mice were maintained on a 16 or 23% fat diet (laboratory chow with added fat, principally corn oil) or on laboratory chow alone (5.5% fat). In another experiment C57BL/1 mice were given a 23% fat diet (as above) or a low-fat diet (67% laboratory chow, 1.9% corn oil, and 31% starch; 5.5% fat). Colon mucosal samples were analyzed for several enzyme activities. In Swiss mice the analyses revealed the following: 1) Ouabain-insensitive ATPase was unaltered in male mice, but it rose significantly in females fed a high-fat diet (this effect was seen when a resuspended high-speed pellet was analyzed but not seen with the initial homogenate); 2) 5'-nucleotidase activity showed a significant stepwise increase with dietary fat; 3) nonspecific esterase activity tended to rise with a high-fat diet (not significant); 4) beta-glucuronidase levels were not altered by diet fat; and 5) ornithine decarboxylase levels were not altered by diet fat. In C57BL/1 mice analyses were done on ouabain-insensitive ATPase, 5'-nucleotidase, nonspecific esterase, and beta-glucuronidase, but no diet effects were seen. Fecal reductase activity was measured with the use of 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride hydrate). A high-fat diet did not affect the activity in C57BL/1 mice, but it caused a significant rise in Swiss mice.
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PMID:High-fat diets and fecal level of reductase and colon mucosal level of ornithine decarboxylase, beta-glucuronidase, 5'-nucleotidase, ATPase, and esterase in mice. 632 44