Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.3.5 (5'-nucleotidase)
 3,167 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

ADP and ATP and their analogues were evaluated as inhibitors of 5'-nucleotidase purified from heart plasma membrane. ADP analogues are more powerful inhibitors than the corresponding ATP analogues. The most powerful inhibitor found is adenosine 5'-[alpha beta-methylene]diphosphate (AOPCP) for which the enzyme shows a Ki of 5 nM at pH 7.2. Measurements of pKi values for ADP and AOPCP as a function of pH indicate that the major inhibitory species of both nucleotides is the dianion. In the physiological range of pH values, AOPCP is a more powerful inhibitor than ADP principally because a higher percentage of AOPCP exists in the dianion form. The methylenephosphonate analogue of AMP (ACP), though not a substrate, is a moderately effective inhibitor. The corresponding analogues of ADP (ACPOP) and ATP (ACPOPOP) are as good inhibitors as ADP and ATP respectively. The thiophosphate analogues of ADP all inhibit 5'-nucleotidase, although not as powerfully as ADP, the most effective of these analogues being adenosine 5'-O-(1-thiodiphosphate) diastereoisomer B (ADP[alpha S](B)]. Other nucleotides inhibit the enzyme, but none is as effective as AOPCP. Inorganic tripolyphosphate and methylenediphosphonate are better inhibitors of the enzyme than is inorganic pyrophosphate. Inorganic thiophosphate is a better inhibitor than is orthophosphate. Hill plots of the ADP and AOPCP inhibition yield slopes close to 1; Hill plots of the ATP inhibition yield slopes of about 0.6. MgADP- is not an inhibitor, and MgATP2- is at best a very weak inhibitor of the enzyme.
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PMID:5'-Nucleotidase from rat heart membranes. Inhibition by adenine nucleotides and related compounds. 298 44

A cell line designated as Ca761-86 has been established from the solid mouse mammary cancer (Ca761) by suspension culture. It has been passaged for more than 200 generations. Moderate round cells were predominant and most of them were mononuclear. Some characteristics of malignant cells and A-type viral-like particles were observed by electron microscopy. The results of cytochemical studies (DNA, RNA, SDH, 5'AMPase, ACP etc) were comparable to the ultramicroscopic results. It multiplied approximately 27.4 fold on Day 5 with mitotic index reaching 1.8% on Day 3. This cell line was a hyperdiploid with karyotype of 45 or 43, -2X, tri12, tri17, +M1-5. Cell agglutination was observed when treated with ConA (greater than or equal to 7 micrograms/ml). Spontaneous agglutination might also take place without adding any ConA. After 5 x 10(6) cells of Ca761-86 suspension were transplanted into the normal inbred 615 mice by different ways (subcutaneous, intrafoot pad or intraperitoneal), the transplantability rate reached 100%. Spontaneous remission was never observed and its metastatic ability reserved. PPLO were not detected. Ca761-86 may be of value for practical purposes.
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PMID:[Establishment of a mammary cancer cell line Ca 761-86 and its biologic characteristics in inbred 615 mice]. 325 Aug 24