Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.3.5 (5'-nucleotidase)
 3,167 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Alkaline phosphatase (ALP), 5'-nucleotidase (5NT), and gamma-glutamyltransferase (GGT) were measured in sera from 335 adult and 93 pediatric inpatients receiving anti-epileptic drugs (AEDs). Among adults, 18 took only phenobarbital, 39 only carbamazepine, 159 only phenytoin, and 99 phenytoin with other AEDs. Of 93 children, 49 took phenytoin, and 44 took another AED. Among the 335 adults, only 118 had normal enzymes, but 316 had 5NT and ALP, each less than two times and GGT less than five times normal limits. Of 19 patients outside these ranges, 14 had either historic, physical, or biochemical evidence of liver or severe multisystem disease. The authors observed that the frequency of enzyme elevations in adults receiving AEDs was lower for 5NT (23%) than for GGT (54%) but similar to ALP (27%). The degree of elevation was also lower for 5NT and ALP than for GGT. Phenytoin was associated with the most frequent and highest enzyme elevations in both adult and pediatric patients. Consideration of these data is necessary in the evaluation of liver function in patients receiving AEDs.
 ...
PMID:Serum 5'-nucleotidase in patients receiving anti-epileptic drugs. 286 88

Biomaterials induce an inflammatory reaction characterized by a rapid recruitment at the implantation site of polymorphonuclear cells and macrophages. In the course of the inflammatory response, the cellular activation triggers expression of a number of enzymes, such as 5'-nucleotidase, which is widely distributed in animal cell membranes as an ectoenzyme. It is now well established that 5'-nucleotidase activity decreases following the contact of inflammatory cells with foreign particles. In this paper we investigate a possible correlation between the enzymatic activities and the dynamic properties of the cell membrane bilayer. Dacron pieces were introduced into rats' peritoneal cavities for a period of 6 h, after which the peritoneal cells were harvested, and various enzyme assays performed, including those for cytoplasmic, lysosomal, and ectoenzymes. In parallel, we studied cell membrane fluidity, using fluorescence polarization of 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH), and cellular ultrastructural alteration resulting from the cell-biomaterial interactions using scanning and transmission electron microscopy. Our results show that: 1) macrophages spread around the Dacron fibers with cytoplasmic finger-like projections, but no phagolysosomes, 2) 5'-nucleotidase levels decrease with surgical trauma in comparison with the resident cell exudate, 3) implantation of biomaterials slightly modify the 5'-nucleotidase levels observed in the sham animal, 4) no differences in the anisotropy values indicating that membrane lipid order within the cells could not account for the observed decrease of 5'-nucleotidase activity. Thus, we can suggest that 5'-nucleotidase expression may reflect a particular feature of cell activation without a phagocytic process.
 ...
PMID:Dacron vascular biomaterial triggers macrophage ectoenzyme activity without change in cell membrane fluidity. 840 21

The human placental microvillar membrane contains several ectoenzymes, including 5'-nucleotidase, alkaline phosphatase and ATP-diphosphohydrolase (ATP-DPH), which might be involved in the extracellular metabolism of nucleotides. The type of anchorage to the plasma membrane of the two first enzymes has been shown to be via a glycosyl-phosphatidylinositol. In the present study, using an enzymatic approach, we show that the ATP-DPH should be attached to the plasma membrane through a different type of anchorage. We were also interested in the search of compounds which could interact differentially with this enzyme to be used as a tool for studying the other two hydrolytic enzymes in the presence of ATP-DPH. Here we report several inhibitors of ecto-ATPases which seem to be a useful tool for studying these three enzymes.
 ...
PMID:Human placental ecto-enzymes: studies on the plasma membrane anchorage and effect of inhibitors of ATP-metabolizing enzymes. 917 64