Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.3.5 (5'-nucleotidase)
 3,167 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

As in rats, administration of estradiol to ovariectomized mice results in a trypsin-like proteolytic activity in the uterus. After fractionation of uteri from estradiol-treated ovariectomized mice the protease activity was found in the 12,000 times g pellet and the nucleus, appearing first in the former. Further fractionation of the pellet by discontinuous sucrose gradient centrigugation resulted in sedimentation of the protease with 5'-nucleotidase, a marker enzyme for plasma membrane and separate from mitochondrial and lysosomal enzyme markers. Solubilization was best accomplished by lysis at 37 degrees. The soluble enzyme from mouse uterus had optimal activity at about 43 degrees and pH 8.3 and was inhibited by diisopropylfluorophosphate, tosylarginine methyl ester, antipain, and leupeptin, but not by soybean trypsin inhibitor. Inhibition in vitro by antipain and leupeptin, two low molecular weight peptides, prompted the study of their effect in vivo on the mouse uterus. After intact, cycling female mice received subcutaneous injections of antipain and leupeptin for 16 days, their uteri showed significant diminution in weight and total DNA when compared to untreated controls. Fertility rates were also diminished. Trypsin-like protease activity may be essential to normal uterine metabolism and function.
 ...
PMID:Antipain and leupeptin restrict uterine DNA synthesis and function in mice. 26 27

1. Rat livers were dissociated into their constituent cells by perfusion through the portal vein with a medium containing collagenase, and hepatocytes separated from non-parenchymal cells. 2. It is shown that the procedure described by Wisher & Evans [(1975) Biochem. J. 146, 375-388] for preparation of plasma membranes from liver tissue when applied to isolated hepatocytes also yielded subfractions of similar morphology and marker-enzyme distribution. 3. Thus the distribution of alkaline phosphodiesterase, 5'-nucleotidase and the basal and glucagon-stimulated adenylate cyclase among two 'light' vesicular and one 'heavy' junction-containing plasma-membrane subfractions paralleled that reported for tissue-derived plasma-membrane subfractions. 4. Increased recoveries and specific activities of plasma-membrane marker enzymes were obtained when soya-bean trypsin inhibitor was included in the collagenase-containing perfusion media used to dissociate the liver. 5. Polyacrylamide-gel-electrophoretic analysis of the corresponding plasma-membrane subfractions prepared from liver tissue and isolated hepatocytes were generally similar. 6. The results indicate that the functional polarity of the hepatocyte's plasma membrane is retained after tissue dissociation. The damage occurring to plasma-membrane ectoenzymes by the collagenase-perfusion procedure is discussed.
 ...
PMID:Preparation of plasma-membrane subfractions from isolated rat hepatocytes. 88 Feb 46

Succinyl trialanine p-nitroanilide [Suc-(Ala)3-pNA] hydrolytic activity, an enzymatic activity related to elastase, in vascular wall was detected and partially characterized. Subcellular distribution of this activity closely paralleled that of plasma membrane marker enzymes, 5'-nucleotidase, and phosphodiesterase I, suggesting its association with the vascular muscle plasma membranes. The same distribution of elastolytic activity was observed. Hydrolytic activity toward Suc-(Ala)3-pNA was inhibited by EDTA, ethyleneglycol-bis(beta-aminoethylether)-N,N'-tetraacetic acid but not by trypsin inhibitor. Enzyme activities were different not only between aortic muscle extracts from young and mature rats, but also among the extracts from elastic and muscular arteries, specific activity being higher in the aortas of young animals or in elastic arteries, respectively. The activity studied [Suc-(Ala)3-pNAase] in vascular wall may play a role in vascular connective tissue metabolism as well as function.
 ...
PMID:Succinyl trialanine p-nitroanilide hydrolytic activity in blood vessels of the rat. 352 26