Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.3.5 (5'-nucleotidase)
 3,167 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The growth of mouse mastocytoma P-815 cells in culture (37 degrees, 42 hr) was inhibited by exogenous adenosine (0.2 to 1.0 mM) and more effectively by AMP (0.01 to 0.1 mM), but not by adenine. The inhibited growth (a 25% inhibition by 0.5 mM adenosine and a 80% inhibition by 0.25 mM AMP) was restored to a near control level by the addition of uridine (0.5 mM) to the medium. The pretreatment (37 degrees, 3 hr) of the cells with adenosine or AMP caused a 60% inhibition of incorporation (37 degrees, 2 hr) of [U-14C]aspartate into uracil nucleotides, accumulating 14C-orotate and orotidine. Both dipyridamole, an inhibitor of adenosine uptake, and exogenous adenosine deaminase suppressed the growth inhibition induced by not only adenosine but also AMP. 2-Chloroadenosine, which is resistant to the action of adenosine deaminase, was a more potent growth inhibitor, while 3'AMP and 2'-AMP, which are not hydrolyzed to adenosine by membrane 5'-nucleotidase, were ineffective. Adenosine 5'-sulfate and other 5'-substituted adenosines were also ineffective. These observations indicate that AMP inhibits the growth of mastocytoma P-815 cells as a result of its continuous conversion to adenosine and a constant exposure of the cells to a low concentration of adenosine which readily permeates the cell membrane. In addition, adenosine, AMP and their agarose-linked forms rapidly (37 degrees, 20 min) elevated cellular levels of cAMP. This effect was not suppressed by dipyridamole. Apparently adenosine and AMP also act extracellularly for growth inhibition by regulating cAMP levels.
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PMID:Effect of adenosine and adenosine 5'-monophosphate on cell division of cultured mastocytoma P-815 cells. 625 14

Activity of 5'-nucleotidase was inhibited in thymus tissue of tumor-bearing mice; bradykinin (1 mg/kg body weight) increased the enzymatic activity 2.0-2.2-fold. Administration of bradykinin led to inhibition of allogenic Crocker's sarcoma growth and to stimulation of syngeneic mastocytoma P-815 growth, which was inoculated into noninbred and BALB/c mice, respectively. Hence, bradykinin activated 5'-nucleotidase in Crocker's sarcoma and did not affect the enzymatic activity in tumor P-815. This suggests that activity of 5'-nucleotidase is of importance in evaluation of the thymus tissue state as well as it demonstrates the tumoral tissue sensitivity to bradykinin.
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PMID:[Activity of 5'-nucleotidase from thymus and tumors upon administering bradykinin to tumor-bearing mice]. 851 76