Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.3.5 (5'-nucleotidase)
 3,167 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

5'-Nucleotidase activity has been localized at the ultrastructural level in the axon-myelin-Schwann cell complex. Sciatic nerves of rabbits of pre- and postnatal development were used. Positive reaction was found on the plasma membrane, basal lamina, cytoplasm, and finger-like processes of the Schwann cells; on the intraperiod lines of the compact myelin, on the surface of myelin sheath, in the split myelin lamellae in the paranodal regions and Schmidt-Lanterman clefts, in segments of outermost and innermost lamellae, split off from the interparanodal myelin, in the mesaxons (outer and inner), in the loose myelin lamellae in the earlier stages of myelinization; on the axolemma (especially in the nodal and paranodal segments), in the periaxonal space, axoplasm. The alterations of 5'-nucleotidase distribution were associated with the developing myelin sheath.
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PMID:Ultrastructural cytochemical localization of 5'-nucleotidase activity in axon-myelin-Schwann cell complex. 284 52

To extend our earlier studies on the subcellular distribution of 5'-nucleotidase in peripheral nerve fibers, we have applied the cytochemical method with cerium ions as trapping agents for inorganic phosphate released from adenosine-5'-monophosphate. Adult and 15-day-old Wistar rats were investigated. In myelinated nerve fibers, 5'-nucleotidase activity was visualized mainly on the Schwann cell plasma membrane, the axolemma, the surface of the myelin sheath and its widened lamellae in the paranodal areas, and Schmidt-Lanterman clefts. Single reaction precipitates were observed at the intraperiod lines of the compact myelin. In addition, in the young animals, the nuclear envelope and the rough endoplasmic reticulum were also found associated with reaction deposits. The simple axon-Schwann cell sheaths of the unmyelinated nerve fibers were also reactive. The cerium-based method reveals a pattern of localization of the 5'-nucleotidase in peripheral nerve fibers quite similar to that detected by the conventional lead-based method. The cerium-based method, however, determines a more uniform distribution of finer reaction deposits along the cellular membranes. In young rats, the enzyme reaction was found to be more intense, especially in the areas of myelin-forming membranes.
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PMID:Electron microscopial localization of 5'-nucleotidase in the rat peripheral nerve fibers by cerium-based cytochemistry. 1097 Jan 25