Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.3.5 (
5'-nucleotidase
)
3,167
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Interest in the role of mononuclear phagocytes in
glomerulonephritis
(GN) and in defining markers of renal neoplasms led the authors to study alpha-naphthyl acetate/butyrate esterase (ANAE/ANBE), alkaline phosphatase (AlkP), acid phosphatase (AcP),
5'-nucleotidase
(5'N), and ATPase (ATP) activity in paraformaldehyde-fixed, plastic-embedded renal tissue from patients with a variety of pathologic conditions. These conditions included GN, renal tumors, and transplant rejection. Enzymatic staining for ANAE, ANBE, AcP, AlkP, and ATP was generally confined to tubules and collecting ducts in normal kidney. Nine of 10 cases of renal carcinoma had weakly to strongly positive reactions with AlkP, AcP, and ANAE; 9 of 10 cases of Wilms' tumor showed weakly positive reactions with AcP and ANAE, particularly in tubular structures. Severely damaged kidney allografts showed surprising retention of normal histochemical features. In all cases 5'N stained both glomerular capillaries and interstitial vasculature; ATPase and AlkP stained interstitial vessels only. Plastic embedding provides superb preservation of both microscopic anatomy and enzymatic activity, which may allow utilization of enzyme histochemistry for diagnostic and research purposes.
...
PMID:Enzyme histochemistry in plastic-embedded sections of normal and diseased kidneys. 258 40
It has been demonstrated in anti-Thy1
glomerulonephritis
that extracellular adenine nucleotides have a significant pro-inflammatory activity, however, glomerular ATP/ADPase, which in concert with
5'-nucleotidase
converts ATP/ADP, and AMP to anti-inflammatory adenosine had an anti-inflammatory role. We have studied distribution of
5'-nucleotidase
and divalent cation-activated ATPase in kidney biopsies of 15 patients with
glomerulonephritis
. The major finding was an overexpression of
5'-nucleotidase
in the mesangium of kidney from patients with membranous nephropathy. No change in
5'-nucleotidase
expression was observed in other common forms of
glomerulonephritis
: IgA nephropathy, mesangioproliferative and mesangiocapillary
glomerulonephritis
. The distribution of Mg(2+)-ATPase in investigated specimens was similar to control distribution. Results obtained in this study indicate increased mesangial expression of
5'-nucleotidase
in non-proliferative form of
glomerulonephritis
consistent to a role of mesangial cells in inflammatory processes.
...
PMID:Increased expression of glomerular mesangial cell 5'-nucleotidase in membranous nephropathy. 1218 7
