Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.3.16 (
calcineurin
)
17,112
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Transmembrane and ubiquitin-like domain-containing 1 (Tmub1) encodes a protein (
TMUB1
) containing an ubiquitin-like domain and plays a negative regulatory role during hepatocyte proliferation, but its mechanism in this process is still unknown. Here,
TMUB1
interfered with the binding of calcium-modulating cyclophilin ligand (CAML) to cyclophilin B, which may represent a key role in the negative regulatory process of
TMUB1
in hepatocyte proliferation. Co-immunoprecipitation assays in rat BRL-3A cells confirmed the interaction between
TMUB1
and CAML; significant regulation of the influx of Ca2+ ([Ca2+]i) and hepatocyte proliferation occurred following
TMUB1
overexpression or knockout. Deletion of the TM1 hydrophobic domain of
TMUB1
completely abolished this interaction and led to loss of
TMUB1
's regulatory effects on cytological behavior. Furthermore, overexpression of
TMUB1
completely abolished the interaction between CAML and its downstream protein cyclophilin B, which can act upstream of
calcineurin
by increasing [Ca2+]i during cell proliferation. Taken together, our results indicate that
TMUB1
regulates BRL-3A hepatocyte proliferation by interacting with CAML and further interferes with the binding of CAML to cyclophilin B to decrease cellular [Ca2+]i.
...
PMID:TMUB1 Inhibits BRL-3A Hepatocyte Proliferation by Interfering with the Binding of CAML to Cyclophilin B through its TM1 Hydrophobic Domain. 2996 78
