Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.3.16 (calcineurin)
 17,112 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Chitin is an essential component of the fungal cell wall and its synthesis is under tight spatial and temporal regulation. The fungal human pathogen Candida albicans has a four member chitin synthase gene family comprising of CHS1 (class II), CHS2 (class I), CHS3 (class IV) and CHS8 (class I). LacZ reporters were fused to each CHS promoter to examine the transcriptional regulation of chitin synthesis. Each CHS promoter had a unique regulatory profile and responded to the addition of cell wall damaging agents, to mutations in specific CHS genes and exogenous Ca2+. The regulation of both CHS gene expression and chitin synthesis was co-ordinated by the PKC, HOG MAP kinase and Ca2+/calcineurin signalling pathways. Activation of these pathways also resulted in increased chitin synthase activity in vitro and elevated cell wall chitin content. Combinations of treatments that activated multiple pathways resulted in synergistic increases in CHS expression and in cell wall chitin content. Therefore, at least three pathways co-ordinately regulate chitin synthesis and activation of chitin synthesis operates at both transcriptional and post-transcriptional levels.
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PMID:The PKC, HOG and Ca2+ signalling pathways co-ordinately regulate chitin synthesis in Candida albicans. 1730 16

Maintenance of the integrity of the cell wall in fungi is essential. One mechanism that cells use to maintain cell wall integrity in response to cell wall damage is to up-regulate chitin synthesis. In Candida albicans, the PKC cell wall integrity, Ca(2+)/calcineurin and high osmolarity glycerol (HOG) signalling pathways co-ordinately regulate chitin synthesis in response to cell wall stress. The transcription factors downstream of these pathways and their DNA binding sites within the promoters of target genes are well characterised in Saccharomyces cerevisiae, but not in C. albicans. The promoters of the C. albicans class I CHS genes (CaCHS2 and CaCHS8) were functionally dissected with the aim of identifying and characterising the transcription factors and promoter elements that mediate the transcriptional up-regulation of CaCHS2 and CaCHS8 in response to cell wall stress. This analysis provided evidence that the PKC cell wall integrity pathway may operate through RLM1-elements in the CaCHS2 and CaCHS8 promoters, but that promoter sequences that respond to the Ca(2+)/calcineurin and HOG signalling pathways in S. cerevisiae did not directly regulate chitin synthase 2 and 8 gene transcription in C. albicans.
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PMID:Dissection of the Candida albicans class I chitin synthase promoters. 1915 67

Although a great deal of progress has been made toward understanding the role of abscisic acid (ABA) in fruit ripening, many components in the ABA signalling pathway remain to be elucidated. Here, a strawberry gene homologous to the Arabidopsis gene ABI1, named FaABI1, was isolated and characterized. The 1641bp cDNA includes an intact open reading frame that encodes a deduced protein of 546 amino acids, in which putative conserved domains were determined by homology analysis. Transcriptional analysis showed that the levels of FaABI1 mRNA expression declined rapidly during strawberry fruit development as evidenced by real-time PCR, semi-quantitative reverse transcription-PCR, and northern blotting analyses, suggesting that the Ser/Thr protein phosphatase PP2C1 encoded by FaABI1 may be involved in fruit ripening as a negative regulator. The results of Tobacco rattle virus-induced gene silencing and PBI121 vector-mediated overexpression suggested that the down- and up-regulation of FaABI1 mRNA expression levels in degreening strawberry fruit could promote and inhibit ripening, respectively. Furthermore, alteration of FaABI1 expression could differentially regulate the transcripts of a set of both ABA-responsive and ripening-related genes, including ABI3, ABI4, ABI5, SnRK2, ABRE1, CHS, PG1, PL, CHI, F3H, DFR, ANS, and UFGT. Taken together, the data provide new evidence for an important role for ABA in regulating strawberry fruit ripening in the processes of which the type 2C protein phosphatase ABI1 serves as a negative regulator. Finally, a possible core mechanism underlying ABA perception and signalling transduction in strawberry fruit ripening is discussed.
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PMID:Type 2C protein phosphatase ABI1 is a negative regulator of strawberry fruit ripening. 2340 98

Sweet cherry (Prunus avium L.) fruits are classified into dark-red and bicolored cultivars based on their anthocyanin contents; however, the mechanisms regulating the accumulation of these pigments are unclear. Here, we reveal that anthocyanin accumulation is highly dependent on light in bicolored 'Rainier' cherries, while it is only slightly light dependent in the dark-red 'Hongdeng' fruits. To reveal the transcriptional mechanisms regulating light-dependent anthocyanin accumulation in bicolored 'Rainier' cherries, we sequenced the transcriptomes of fruits grown in light or in darkness. Genes encoding the anthocyanin biosynthesis enzymes chalcone synthase, chalcone isomerase, flavanone 3-hydroxylase, and flavonoid 3'-hydroxylase were significantly upregulated by light in the bicolored fruits. Most of the differentially expressed regulatory genes were known to be involved in the light or hormone signal transduction pathways, such as those encoding protein phosphatase 2Cs, PHYTOCHROME INTERACTING FACTOR 3, phytochromes, and ELONGATED HYPOCOTYL 5. The expression levels of 32 highly expressed transcription factors were found to be significantly altered by light in the bicolored fruits, including members of the basic leucine zipper, R2R3-MYB, and WRKY transcription factor families. A co-expression network analysis further revealed that many of the light-regulated genes were co-expressed with genes involved in the abscisic acid and gibberellic acid signaling pathways, suggesting that these phytohormones play important roles in light-dependent anthocyanin biosynthesis. Together, our data reveal multiple roles for light in regulating anthocyanin biosynthesis in differently colored cherries.
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PMID:Transcriptomic analysis of light-dependent anthocyanin accumulation in bicolored cherry fruits. 3013 Dec 7

The influences of abscisic acid (ABA)-guanosine 3',5'-cyclic monophosphate (cGMP) on UV-B treatment-stimulated isoflavone synthesis in soybean sprouts was explored. It turned out that ABA, with cGMP, up-regulated gene expression and activity of chalcone synthase (CHS) and isoflavone synthase (IFS), and subsequently induced isoflavone biosynthesis under UV-B treatment. Furthermore, data obtained from the isobaric tags for relative and absolute quantification (iTRAQ) analysis showed that there were two core components in ABA response: SNF1-related protein kinase (SnRK) and type 2C protein phosphatase (PP2C), were up and down regulated after UV-B treatment, respectively. UV-B exposure stimulated increment in guanine nucleotide-binding protein and calreticulin expression. Additionally, CHS and IFS protein expression were up regulated under UV-B stress. Overall, UV-B-induced ABA resulted in PP2C inhibition and SnRK2 activation, and up-regulated CHS and IFS expression, leading to enhancement of isoflavone accumulation. cGMP and calreticulin as downstream messengers, mediated ABA-stimulated isoflavone biosynthesis after UV-B exposure.
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PMID:Cyclic GMP mediates abscisic acid-stimulated isoflavone synthesis in soybean sprouts. 3072 18