Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.3.16 (
calcineurin
)
17,112
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The cold-induced antisense transcript
COOLAIR
represses
FLOWERING LOCUS C
(
FLC
) transcription with increased H3K27me3 and decreased H3K36me3 levels in response to cold temperatures. However, the molecular connection between
COOLAIR
and histone modification factors in the absence of cold treatment remains unclear. We report that the RNA binding protein FCA interacts with the PRC2 subunit CURLY LEAF (CLF) and binds nascent
COOLAIR
transcripts to allow deposition of H3K27me3 at
FLC
. Loss of
COOLAIR
function results in a reduction in FCA and CLF enrichment, which, in turn, decreases H3K27me3 levels at
FLC
. The
Arabidopsis
protein phosphatase
SSU72
physically interacts with the RRM1 motif of FCA to antagonize FCA binding with
COOLAIR
. Mutations in
SSU72
caused early flowering, reduced
FLC
transcription, increased CLF enrichment and H3K27me3, and enhanced affinity between FCA and
COOLAIR
. Our results suggest that FCA binding of
COOLAIR
and
SSU72
is critical for PRC2 enrichment and H3K27me3 deposition in
Arabidopsis
.
...
PMID:PRC2 recruitment and H3K27me3 deposition at
FLC
require FCA binding of
COOLAIR
. 3103 1
