Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In 50 workers of a non-iron metallurgic plant exposed in their work to lead and trace amounts of cadmium and zinc cytochemical reactions were carried out in peripheral blood granulocytes. The control group comprised 30 men. In the investigations the degree of intoxication with lead compounds and the effect of the length of exposure ion the cytochemical reactions were taken into account. It was observed in these investigations that chronic exposure to lead and trace amounts of the remaining elements caused changes in the activity of acid phosphatase, alkaline phosphatase, lactic dehydrogenase and MOP. In particular, a significant fall of MOP and acid phosphatase activity and increased LDH activity in subjects exposed to lead with biochemical evidence of lead absorption deserve attention. With longer exposure to lead the activity of MPO decreased in workers with evidence of lead absorption.
Acta Haematol Pol
PMID:[Cytochemical studies of peripheral blood granulocytes of workers with occupational exposure to lead compounds]. 627 22

Swiss mice were irradiated with X-rays (5, 7 and 9 Gy) and neutrons (3 and 5 Gy) determining the degree of 3H-thymidine incorporation into the cells in the intestinal crypts, alkaline phosphatase activity in intestinal homogenate and serum, and radiation-induced leucopenia. It was shown that damage to the cells in intestinal crypts depends on the dose and type of radiation. More radiation-resistant cells covering the intestinal villi showed a significant inhibition of alkaline phosphatase activity at high radiation doses. Changes in intestinal alkaline phosphatase activity were reflected also by changes in its level in the serum where its dependence on the dose of radiation was evident also.
Acta Physiol Pol
PMID:Regeneration in small intestine following exposure to X-radiation and neutron beam as estimated in the light of changes in alkaline phosphatase activity and degree of 3H-thymidine incorporation into cells in intestinal crypts. 653 77

Twenty Thoroughbred 3 year old horses (10 stallions and 10 mares), trained and raced at the Warsaw Race-Course were studied from March through November. Blood was taken approximately every 8 weeks to determine the activities of aspartate and alanine transaminases, acid and alkaline phosphatases and aldolase. It was observed that the activities of aspartate aminotransferase and alkaline phosphatase reached their maxima in July and alanine transaminase in May. The activities of acid phosphatase and aldolase showed their minima in July. Comparing these data with the literature it was noted that the changes observed are mainly seasonally-dependent; but, training had some influence on the activity of the enzymes involved in energy metabolism.
Acta Physiol Pol
PMID:Seasonal enzyme activity changes in two aminotransferases AspAT and AlAT, acid and alkaline phosphatases and aldolase in the serum of Thoroughbred horses during a racing season. 653 19

The aim of this work was to find out the histochemical activity of alkaline and acid phosphatases, and nonspecific esterases in intestinal mucosa in spontaneous swine colibacillosis. The investigated animals were divided into 3 groups: I--oedema disease (12 pigs), II--enetrotoxemia due to E. coli (8 pigs), III--control animals (5 pigs). The above grouping was based on the clinical, bacteriological and post-mortem examination. A slight increase of alkaline phosphatase activity in the jejunum and ileum of sick pigs was observed. The acid phosphatase activity decreased in the duodenum of oedema diseased pigs, while it increased in the cecum and colon--comparing with the control and enterotoxemia groups of animals. The decrease of acid phosphatase activity was observed in the jejunum and ileum of the enterotoxemia group of animals. The difference in the activity of nonspecific esterases concerned only the cecum and colon of diseased pigs, where its decrease was observed.
Pol Arch Weter 1982
PMID:[Activity of various enzymes of the intestinal mucosa in spontaneous colibacteriosis in pigs]. 676 35

1. Acid phosphatase (AcPase) from potato tubers was purified by tannic acid fractionation, DEAE-cellulose chromatography, filtration on Bio-Gel P-150 and affinity chromatography on Con A-Sepharose. The enzyme was purified 260-fold and was electrophoretically homogeneous; its mol. mass is about 69 000. 2. The carbohydrate component accounts for 16.6% of the total enzyme weight and includes mannose (5.6%), rhamnose (3.4%), glucose (2.5%), galactose (1.5%) and glucosamine (3.6%). In the amino acid composition aspartic acid, glutamic acid, serine and glycine account for 37.7% of total amino acid residues. 3. Optimum pH is at 5.0-5.3. The enzyme activity was reduced by half after 30 min incubation at 60 degrees C, and was fully abolished after 2 h incubation at 70 degrees C. The enzyme is a nonspecific phosphomonoesterase; aromatic phosphomonoesters and inorganic pyrophosphate can serve as substrates. Apparent Km values were 1.25 mM and 40 mM for p-nitrophenylphosphate and inorganic pyrophosphate, respectively. The enzyme is inhibited by MoO42-, Zn2+, Hg2+ and urea. Inhibition caused by urea was reversible at urea concentration below 9 M.
Acta Biochim Pol 1982
PMID:Acid phosphatase of potato tubers (Solanum tuberosum L). Purification, properties, sugar and amino acid composition. 715 77

The activity of both enzymes was measured in the extracts of all parts of the reproductive system of the hen. Very high activity of alkaline phosphatase was found in the follicle membranes and very low values in the oviduct. The values of the activity of acid phosphatase are similar in various parts of the oviduct and ovary. Only in the contents of the follicle and in the yolk the activity was near zero. The electrophorograms of alkaline and acid phosphatases in the ovary and the oviduct show similar phenotypes as in the blood. It seems that acid phosphatase does not play any topical role in the functions of the reproductive system. Very high activity of alkaline phosphatase, particularly in the membrane of the ovarian follicles, confirms the suggestion that this enzyme has an active role in the transport of yolk material from the blood into the follicle during its growth.
Acta Physiol Pol
PMID:Activity and electrophoretic forms of alkaline and acid phosphatases in the reproductive system of laying hen. 718 9

The interactions between zinc and lead in their effect on the biochemical and histochemical activity of alkaline phosphatase were investigated in different parts of the small intestine in rats: in the duodenum, jejunum and ileum, as well as in the liver, kidney and serum. The animals were divided into three experimental groups receiving during 120 days 50 micrograms/ml Zn, 100 micrograms/ml Pb and 50 micrograms/ml Zn + 100 micrograms/ml Pb in deionized water. In the duodenal mucosa in all experimental groups the activity of alkaline phosphatase was raised in relation to controls. The administration of both metals separately increased also the activity of the enzyme in the jejunal mucosa but their joint administration decreased this activity by 28% in relation to the control group. The activity of the enzyme in the ileal mucosa was decreased in all experimental animals. In the homogenates of the liver and kidneys of the rats given only zinc a statistically significant rise was found in the activity of the enzyme. In the remaining experimental groups this activity was decreased. Both metals given separately or jointly increased significantly the activity of the enzyme. Moreover, administration of lead caused a change in the location of alkaline phosphatase in the kidney shifting it towards the basement membrane of the epithelial cells in the convoluted tubules.
Acta Physiol Pol
PMID:Zinc and lead interactions on alkaline phosphatase activity (E.C. 3.1.3.1.) in rats. 718 48

The activity of serum alkaline phosphatase in pigs changes in relation to the age of the animals [2, 4, 5, 7]. In chicken [3] it was observed that the activity of alkaline phosphatase showed cyclic fluctuations during the process of egg formation, but in the 24 hours day cyclic the activity of the enzyme was not increasing as far as the process of shell calcification was not taking place. In the presently reported work circadian fluctuations of alkaline phosphatase activity were investigated in the serum of piglets.
Acta Physiol Pol
PMID:24 hours day cycle variations of serum alkaline phosphatase activity in piglets. 723 38

The inhalation of furfural, 20 mg/m3 by adult rats during 3 months for 5 h daily, 6 times a week, did not change the calcium and phosphorus level in blood serum and in bones, but depressed the activity of alkaline phosphatase in blood serum. The exposure to furfural of immature rats (6-7 weeks old) during 4 weeks caused a depression of blood serum and intestine mucosa alkaline phosphatase activity, elevation of calcium and phosphorus levels in blood serum with concomitant fall in bones, and a decrease in calcium excretion with urine.
Pol J Pharmacol Pharm
PMID:The influence of furfural inhalation poisoning on calcium and phosphorus metabolism in rats. 731 18

The reported investigations were carried out on female Wistar rats which were given at 72-hour interval during 117 days (40 doses) dichlorphos subcutaneously, intragastrically and percutaneously, and chlorphenvinphos, bromphenvinphos, methylbromphenvinphos and phospholine subcutaneously in doses amounting to 5% of LD50. After the 40th dose the animals were weighed and killed, blood and organs were taken for investigations. The activity of AChE was determined in the erythrocytes, brain, tibialis muscle and liver. In the serum the levels of transaminases (AspAT, AlAT), alkaline phosphatase, cholesterol and phospholipids were determined. Histomorphological and histochemical investigations included the liver, kidneys, myocardium, femoral muscle, diaphragm, hypothalamus, gastric wall and abdominal sympathetic ganglia. A statistically significant fall of body weight was observed in all intoxicated animals as compared with controls. The mean AChE activity was about 50% of the activity in the control group. A decrease was found also in the cholesterol-phospholipid ratio in the intoxicated animals. Degenerative changes in the parenchymal organs developed independently on the route of poison administration and they included particularly the liver (atrophy of parenchyma) and myocardium (hyperaemia, extravasations, early necrotic changes of muscle fibres, nuclear pyknosis). Necrosis and oedema were observed also in the neurons of sympathetic ganglia. The greatest intensity of the lesions was found after intoxication with the agents which were most potent AChE inhibitors (phospholine and chlorphenvinphos).
Acta Physiol Pol
PMID:Activity of certain enzymes and histomorphological changes in subacute intoxication of rats with selected organophosphates. 733 85


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