Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Administration of pesticides to the experimental animals bring changes in the activity of enzymes: 3.1.3.1. alkaline phosphatase (AP), 3.1.3.2. acid phosphatase (AcP), 1.4.1.2. glutamin dehydrogenase (GDH), 1.1.1.27. lactate dehydrogenase (LDH), 2.6.1.1. glutamic oxalacetic transaminase (GOT), and 2.6.1.2. glutamic pyruvic transaminase (GPT), determined in blood serum, liver, and kidneys. In the majority of cases these changes are statistically significant.
Pol J Pharmacol Pharm
PMID:The influence of carathane and toxaphene on the activity of some enzymes in rat's tissues in the studies in vivo. 116 18

Post-mortem examinations concerned 16 pigs which had died of the oedema disease. A group of 8 healthy pigs were slaughtered at the Lublin abattoir and used as controls. All animals were subjected to detailed autopsy, and sections of the liver were taken for histological examination. The fixed material was cut into sections by the paraffin and refrigeration methods. To the routine staining with haematoxylin and eosin, colour reactions were added to reveal glycogen, simple fats and acid and alkaline phosphatase. The macro- and microscopic examinations demonstrated chronic, focal inflammation of the liver capsule connected with liver cirrhosis. Hyperaemia of the liver was noticed in all cases. A decrease of glycogen, associated with progressive fatty infiltration of the liver cells, was observed in the diseased pigs in contrast to the control group. An increase of the activity of hydrolytic enzymes and a positive reaction to cholesterol compounds are symptoms of progressive catabolic changes in the liver cells.
Pol Arch Weter 1975
PMID:[Estimation of pathomorphological and histochemical changes in the liver of pigs in the course of edema disease of swine]. 118 54

In 47 patients with hepatocellular or obstructive jaundice and 20 healthy controls the alkaline phosphatase isoenzyme pattern was determined by means of electrophoresis on polyacrylamide gel. It was demonstrated that the main activity of alkaline phosphatase (AP) in healthy subjects was connected with beta globulins while beta lipoproteins contained only traces of this activity. In hepatocellular jaundice during viral hepatitis only a slight rise was found of the activities of isoenzymes present in healthy subjects, and no zones of additional activity were found. In obstructive jaundice of benign as well malignant etiology the beta lipoprotein zone increased also significantly, moreover an additional activity zone was revealed moving in the gel together with alpha2macroglobulins. In 40% of cases of obstructive jaundice caused by metastatic malignant neoplasms in the liver a 4-fraction isoenzyme pattern of AP was demonstrated with additional activity in the haptoglobin zone.
Pol Med Sci Hist Bull
PMID:Non-homogeneity of serum alkaline phosphatase activity in certain diseases of the liver and bile ducts investigated by polyacrylamide electrophoresis. 122 17

In 50 patients with mitral stenosis in the stage of maximal clinical improvement a correlation between the functional state of liver and the morphological changes was estblished. For this purpose the serum total bilirubin concentration was determined together with the serum total protein, albumin and globulin levels, serum alanine aminotransferase, aspartate aminotransferase, cholinesterase, leucylaminopeptidase, and alkaline phosphatase activities. The bromsulphalein test, provoked hypoprothrombinemia test, and histological examination of liver biopsy specimens were made. It was found that with increasing liver morphological lesions there is a gradual but not parallel impairment of function. The most sensitive index of hepatic functional disturbances was the test of provoked hypoprothrombinemia according to Kirchmayer and Bromowiczowa. For full assessment of the degree to which the changes in the congested liver have advanced it is necessary to perform liver function tests in combination with histological examination of the organ.
Pol Med Sci Hist Bull
PMID:Correlation of liver function and morphological abnormalities in mitral stenosis. 122 31

Rats kept on a low-magnesium diet for 14 weeks developed increasing clinical symptoms, from slight trophic changes to inhibition of growth. Focal fragmentation of myocardial fibers was followed by focal fuchsinophilia and later--by focal necrosis. Increasing morphological lesions were associated with calcium salts deposition in the degenerated myocardial fibers, connective tissue and wall of cardiac blood vessels. The low-magnesium diet did not affect the activity of ATP-ases and cytochrome oxidase. The areas of myocardial degeneration and necrosis were free of the activity of alkaline phosphatase and succinic dehydrogenase.
Pol Med Sci Hist Bull
PMID:Effect of low-magnesium diet on the histology and the activity of certain enzymes of rat myocardium. 122 2

Among 179 patients with various chronic diseases of the liver the authors found in 20 cases histological evidence of intrahepatic cholestasis and in all these cases lipoproteid X (LP-X) was present in the serum. In the group of 159 patients without evidence of cholestasis in microscopic examination of liver tissue LP-X was demonstrated only in two cases (1.3%). The results of investigations confirm the view that detection of LP-X in the serum is a specific and sensitive index of cholestasis and its value in the diagnosis of this condition is greater than that of determinations of alkaline phosphatase, gamma-glutamyl-transpeptidase, leucinamino-peptidase activity or cholesterol in the serum.
Pol Med Sci Hist Bull
PMID:Significance of serum lipoproteid-X (LP-X) determination for the diagnosis of cholestasis in chronic liver diseases. 122 4

Cytochemical changes were studied in leukocytes in peripheral blood smears from rabbits chronically exposed to mercury vapor. Experimental animals were exposed in a toxicologic chamber to air containing metallic mercury in concentrations of 2.0-2.5 mg/m3 for 3 hours daily over 12 weeks. In the poisoned rabbits, as compared with controls, alkaline phosphatase activity was depressed in granulocytes, and lactate dehydrogenase activity in granulocytes and lymphocytes. The activities of acid phosphatase, arylsulphatase, 5'-nucleotidase, the color reaction with Sudan black B and the p.a.S. reaction were not affected.
Pol Med Sci Hist Bull
PMID:Cytochemical abnormalities of the leukocytes of peripheral blood of rabbits in chronic experimental intoxication with mercuric vapors. 122 12

The protein content and activity of enzymatic markers of cell organelles: succinate dehydrogenase, glucose-6-phosphatase, uricase, acid phosphatase, 5'-nucleotidase and alkaline phosphatase were assayed in the homogenate and the supernatant (after two-hour centrifugation at 140,000 X g) of the liver and intestinal epithelium in rabbits irradiated with a single dose of 550 rads of gamma rays. The determinations were carried out on 1,3,6,9,15 and 30 days after irradiation for experimental and control animals. After gamma irradiation the following alterations were found: 1) increase in protein content (marked between 3-6 days), 2) remarkable rise of alkaline phosphatase activity (during the entire period of study), 3) elevation of 5'-nucleotidase activity (only in the intestinal epithelium), 4) marked reduction of succinate dehydrogenase and uricase activity (on the first day of study), 5) moderate decrease of glucose-6-phosphatase activity (mainly on the third day). Apart from a slight decline in the activity of acid phosphatase in the homogenate of intestinal epithelium, on the third day there practically were no changes in the activity of this enzyme either in the supernatant of intestinal epithelium or in the liver tissue.
Pol Med Sci Hist Bull
PMID:Effect of gamma radiation on the enzymatic activity of cell organelles of liver and epithelium of small intestine in rabbits. 123 88

Some lectins were used to study the localization of sugar residues on the endothelial cell surface in the pia mater blood vessels of control (WKY) and hypertensive rats (SHR). The lectins tested recognized the following residues: beta-D-galactosyl (Ricinus communis agglutinin 120, RCA-1), alpha-L-fucosyl (Ulex europaeus agglutinin, UEA-1), N-acetylglucosaminyl and sialyl (Wheat germ agglutinin, WGA), N-glycolyl-neuraminic acid (Limax flavus agglutinin, LFA), and N-acetyl-D-galactosaminyl (Helix pomatia agglutinin, HPA). Several differences were revealed in the presence of sugar receptors on the surface of endothelial cells between the control and the hypertensive rats. Our studies showed also differences in the localization of the tested glycoconjugates between pial capillaries, small, medium-size and large pial arteries. The histochemical evaluation of alkaline phosphatase revealed an increased activity of the enzyme in the pial vessels of SHRs as compared with control rats with a similar localization of the enzyme activity. Some differences in the distribution of lectin binding sites and alkaline phosphatase activity could be associated with the different functions of particular segments of the pial vascular network.
Neuropatol Pol 1992
PMID:Lectin histochemistry and alkaline phosphatase activity in the pia mater vessels of spontaneously hypertensive rats (SHR). 129 33

31 adult patients (15 male and 16 female) with chronic renal failure were treated for 6 months with 1-alfa-hydroxycholecalciferol on a dose 0.25-2.0 micrograms/24 h. 15 patients with not very advanced renal failure (serum creatinine level 176.8-442 mumol/l) received conservative therapy (group I), 16 patients with serum creatinine value 884-1326 mumol/l were treated by intermittent hemodialysis (group II). The statistically significant decrease of serum alkaline phosphatase activity in group I and II (p < 0.01), the rise of serum calcium level in group I (p < 0.005) were determined. Half of the patients from both the groups stated the relief or disappearance of bone and joint pains and muscle weakness. Besides in group I significant decrease of creatinine clearance (p < 0.001) and increase of serum urea and creatinine value (p < 0.01) were noticed. On the basis of these results we can conclude that the treatment with 1-alfa-hydroxycholecalciferol, produced by "Polfa", ought to be introduced gradually with increasing doses and frequent monitoring of calcium-phosphate metabolism and renal function parameters.
Pol Tyg Lek
PMID:[Clinical estimation of 1-alpha-hydroxycholecalciferol in treatment of patients with chronic renal failure]. 130 33


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