Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Soluble alkaline phosphatase from Thiobacillus thioparus cells was purified about 230-fold. The enzyme had a mol. wt. of 50 000 daltons, optimum pH at 10.5, and was heat-resistant in the presence of diethanolamine. Polyacrylamide-gel electrophoresis demonstrated contamination of the preparation with inactive proteins and the presence of two active bands. The enzyme activity was distinctly stimulated by increasing concentrations of Tris or diethanolamine. In the presence of glycine, 1 mM-Zn2+ enhanced the enzyme activity; in Tris or diethanolamine buffers the activity was stimulated by 1 mM-Mg2+ whereas Zn2+ had a strong inhibitory effect. Glycine at concentrations exceeding 25 mM also inhibited the enzyme. Specificity of the enzyme is fairly broad.
Acta Biochim Pol 1976
PMID:Alkaline phosphatase of Thiobacillus thioparus. Partial purification and properties of the enzyme. 0 90

Activity of alkaline phosphatase as well as its substrate specificity in Vibrio el tor and in nitrofurantoin resistant Vibrio el tor have been studied. A lower level of activity is observed in Vibrio el tor after its acquisition of resistance towards nitrofurantoin. The enzyme activity in both the strains is significantly inhibited by EDTA, and also by metal ions like Mg++, Zn++ and Mn++. The normal strain is found to possess two isoenzymes for alkaline phosphatase whereas the resistant strain has only one isoenzyme.
Acta Microbiol Pol 1978
PMID:Studies on alkaline phosphatase in normal and nitrofurantoin resistant Vibrio el tor. 8 86

In 317 patients with diseases of the liver and bile tract the authors determined LP-X in the serum and the obtained results were compared with the morphological findings in liver tissue or intraoperative examination of the extrahepatic bile ducts. Int he same patients serum bilirubin, total cholesterol, alkaline phosphatase activity, gamma-glutamyltranspeptidase and leucylaminopeptidase activity were determined. In the light of these results the authors believe that detection of LP-X in the serum is a sensitive and specific method in the diagnosis of cholestasis and that it is of greater usefulness than determination of alkaline phosphatase activity. Determinations of other biochemical parameters is of small diagnostic importance in these cases.
Mater Med Pol
PMID:Diagnostic importance of lipoprotein-X (LP-X) in the diagnosis of cholestasis. 17 22

Thirty-five ovarian tumors were examined (7 granulosa-cell tumors, 2 thecomas, 4 dysgerminomas, 6 mucinous cystadenomas, 10 serous cystadenomas, 2 adenocarcinomas, 3 Krukenberg tumors and 1 early embryonic tumor). The granulosa-cell tumors showed high activity of alpha-glycerophosphate dehydrogenase, and the changed cells of their stroma--that of glucose-6 phosphate and isocitrate dehydrogenases. The activity of the latter enzymes was striking also in thecomas. Dysgerminomas showed high alkaline phosphatase, acid phosphatase and nonspecific esterase activities. The authors emphasize the role of stromal cells in the hormonal activity of ovarian neoplasms.
Pol Med Sci Hist Bull
PMID:Histochemistry of hormonally active ovarian tumors. 17 58

The effects of sequential therapy (mestranol alone, and combined with norethisteron acetate and chlormadinon acetate) on liver functions were studied in 2 groups of healthy females (16-32 years of age): the first group consisted of 15 subjects who were given 0.08 mg/day mestranol from the 5th to the 24th day of the cycle and 1 mg/day norethisterone acetate from the 15th to the 24th day of the cycle; the second group of 15 subjects were given 0.08 mg per day mestranol from the 5th to the 24th day of the cycle and 2 mg/day chlormadinon acetate from the 15th to the 24th day of the cycle. Blood samples were collected and Ujoviridine tests (indocyanine green tests) were done 3 times under standarized conditions in both groups during the 1st treatment cycle. Comparison of the 2 forms of sequential treatment using simple variance analysis showed no significant differences between the 2 gestagen phases (norethisteron acetate and chlormadinon acetate) or between the alkaline phosphatase and aminotransferases (the values for these 2 parameters however dropped after norethisterone acetate and rose after chlormadinon acetate). This was attributed to the observation that the 10-day estrogen phase followed by an estrogen-gestagen phase of identical length closely approximate the physiological cycle. The enzymatic results and the ICG half-life indicate that both sequential treatments have no serious toxic effects on the liver functions of healthy fertile women.
Mater Med Pol
PMID:Studies on liver functions under the effect of sequence drugs. 48 Sep 80

1. An endonuclease has been isolated from the nuclei of rye (Secale cereale L) germ and partially purified. The enzyme shows optimum activity over the pH range 5.4-7.4 towards both DNA and RNA, and has no phosphomonoesterase or phosphodiesterase activity. 2. DNA is degraded by the rye germ nuclease to oligonucleotides of similar size, and RNA to oligonucleotides and mononucleotides containing a C-terminal 5'-phosphate group. 3. The rate of hydrolysis of nuclear acids by the enzyme decreases in the following order: native DNA greater than denatured DNA greater than RNA. Synthetic polynucleotides are hydrolysed at a rate decreasing in the order: poly(A) greater than poly(U) greater than poly(C) greater than poly(G).
Acta Biochim Pol 1977
PMID:Purification and some properties of a nuclease from rye germ nuclei. 61 Feb 81

The activity of some enzymes of neutrophils in peripheral blood of rats in subacute benzene vapour poisoning (an exposure to the concentration of benzene vapour of 27.000 mg/m3, 6 hr daily for 10 consecutive days) was determined cytochemically. Increase in the activity of alkaline phosphatase and decrease in that of acid hydrolases: acid phosphatase, beta-glucuronidase and beta-glucosaminidase were found. The results obtained indicate destructive action of benzene on lysosomes of peripheral blood neutrophils.
Pol J Pharmacol Pharm
PMID:Enzymatic changes in peripheral blood leukocytes in rats in subacute benzene vapours poisoning. II. The activity of neutrophils enzymes. 74 May 49

Using cytoenzymatic methods the activity of acid and alkaline phosphatase, non-specific esterase and glycogen content in peripheral blood cells were analyzed. A statistically significant rise in the activity of these enzymes was demonstrated in granulocytes and lymphocytes and a low level of glycogen in lymphocytes. Following prednisone treatment a statistically significant decrease was observed in the activity of studied enzymes and a rise in glycogen content.
Pol Med Sci Hist Bull
PMID:Cytochemical studies of peripheral blood granulocytes and lymphocytes in patients with systemic lupus erythematosus. 95 49

The NBT reduction test and determination of alkaline phosphatase activity in the peripheral blood granulocytes (FAG) were done in 94 subjects including 30 blood donors donating blood for the first time and 64 cases of various haematological syndromes. Raised proportion of formazan granulocytes was found in patients with pancytopenia, acute myeloid leukaemia, chronic myeloid leukaemia during blastic exacerbation, Hodgkin's disease during exacerbation and lymphosarcoma. These results correlated with increased FAG activity. Lower proportions of formazan granulocytes capable of spontaneous reduction of NBT were found in patients with chronic myeloid leukaemia, in immunohaemolytic anaemias and in plasmocytoma. Of all the above syndromes only in chronic myeloid leukaemia impaired ability of formazan cell formation parallelled decreased FAG activity. In the remaining syndromes FAG activity in the granulocytes was normal or raised. In the remissions of Hodgkin's disease a fall was observed in the proportion of formazan granulocytes to values of FAG. In chronic myeloid leukaemia the proportion of formazan cells showed considerable fluctuations and no correlation was observed between the proportion of formazan cells and FAG activity.
Acta Haematol Pol
PMID:[Spontaneous nitroblue terazolium reduction test (NBT) by peripheral blood granulocytes in healthy subjects and in some hematologic syndromes]. 105 43

1. Wheat shoot phosphotransferase has been employed, with p-nitrophenylphosphate as a phosphate donor, to specifically phosphorylate the 5'-position of a variety of nucleosides and nucleoside analogues. The specificity of the enzyme towards the 5'-position of pentose nucleosides is testified to by the complete resistance to phosphorylation of 5'-O-methylcytidine. 2. With the use of ion-exchange chromatography, the foregoing procedure has been applied to the large-scale preparation of nucleoside-5'-phosphates with overall yields of the order of 80-90%. Quantitative recovery of unreacted nucleoside makes it possible to use this method without risk of losses either on a small or large scale with rare nucleosides. It is also applicable to acid- and alkali-labile nucleosides which cannot readily be phosphorylated by chemical procedures. 3. The wheat shoot phosphotransferase also phosphorylated a galactopyranosyl nucleoside, as well as such derivatives as 1-(beta-hydroxyethyl)cytosine and 5-(beta-hydroxyethyl)uracil, showing that the enzyme does not have an absolute requirement for a 5-membered sugar ring, but rather for the presence of a primary hydroxyl group. 4. The phosphorylated derivatives of galactopyranosyluracil, and of both hydroxyethyl pyrimidines, were resistant to 5'-nucleotidase. E. coli alkaline phosphatase converted all three nucleotides quantitatively to the starting compounds. 5. A synthesis of 1-(beta-hydroxyethyl)cytosine is described.
Acta Biochim Pol 1975
PMID:Preparative enzymic synthesis of nucleoside-5'-phosphates. 109 45


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