Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Allergen B previously isolated from honeybee venom and shown to be a mildly acidic protein consisting of polymers of a chain of 49,000 d is shown to have acid phosphatase activity. Allergen B is homogeneous by several criteria. No acid phosphatase, alkaline phosphatase, or esterase activity was found in any other allergen or fraction of bee venom. Acid phosphatase activity was also found in yellow jacket venom and extracts of venom sacs from bumblebees and paper wasps.
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PMID:Allergens in bee venom. III. Identification of allergen B of bee venom as an acid phosphatase. 85 76

50 guinea pigs were allergized three times in 3 days intervals by subcutaneous injection of 25% solution of egg-white in physiological saline in a dose of 0-1 ml/100 g of body weight. On the 21 day after the last injection the animals were exposed to aerosol of antigen of egg-white. 11 animals died in acute anaphylactic shock. The control group consisted of 12 guinea pigs which received subcutaneously a solution of physiological sodium chloride of the same dosis--0-1 ml/100 g of body weight and were also exposed to the allergen, together with the experimental group. The removed parathyroids together with the thyroid gland were studied with histologic and cytoenzymatic methods. The activity of alkaline and acid phosphomonoesterase, nonspecific AS-naphtol acetate esterase, succinic (SDH), lactic (LDH) and D-L-alfaglicerophosphate dehydrogenase (alpha-GPD) were tested. No morphological changes in the parathyroids of guinea pigs in anaphylactic shock were found. Instead a decrease of the enzymatic activity of dehydrogenases was found, what might be connected with the decrease of metabolic activity of the cells. The decrease of alkaline phosphatase activity in the endothelial cells of the capillaries was another finding. It is likely that in an acute anaphylactic shock in guinea pigs only functional changes develop which have not counterparts in histology visible under the light microscope.
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PMID:Cytoenzymatic investigations of parathyroid glands in acute anaphylactic shock of guinea pig. 86 38

Twenty days after the onset of alloxan-induced diabetes, a villous hyperplasia has developed in the intestines of rats having free access to food. The transformation is characterised by a considerable increase in the area of the villous surface, caused by an enhanced mitotic activity in the crypts. The absorption of glucose or methionine by jejunal loops, whether expressed in terms of serosal area or villous area, is unchanged at this stage. On the other hand, the specific activity of certain disaccharidases and dipeptidases in crude mucosal homogenates is greater in diabetic animals, but quantitative histochemistry revealed no changes in the activities of alkaline phosphatase, leucine amino-peptidase and non-specific esterase in the individual enterocytes. Thus the biochemical changes may simply reflect the hyperplasia of the mucosa. The blood sugar level does not appear to be directly responsible for the mucosal transformation; however, the positive correlation between the daily food intake and the villus height suggests a role of hyperphagia and consequent increased luminal nutrition in the development of the hyperplasia.
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PMID:Structural and functional studies on the transformation of the intestinal mucosa in rats with experimental diabetes. 88 18

At 20 degrees C, aflatoxin B1, at a sublethal dose, decreases the activity of alkaline phosphatase (EC 3.1.3.1), alpha-glucosidase (EC 3.2.1.20), esterase (EC 3.1.1.1), chymotrypsin (EC 3.4.21.1), leucine aminopeptidase (EC 3.4.11.1), and phosphoamidase (EC 3.9.1.1) biosynthesis in Bacillus thuringiensis (Berliner). In contrast, at 41 degrees C no significant decrease was observed. At this temperature, the mycotoxin is not destroyed or metabolized and bacterial cells are resistant to the toxin.
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PMID:[Effect of aflatoxin B1 on the enzymatic activities of Bacillus thuringiensis (Berliner)]. 88 28

The influence of antidiuretic hormone (ADH) and papaverine on hydroxyquinoline-induced nephropathy in rats was tested, using histotopochemistry, enzyme activity measurement and morphometric investigation. Hydroxyquinoline causes a marked increase in renal weight, the development of wedge-shaped foci with severely dilated tubule segments, and a simultaneous reduction in dehydrogenases, alkaline phosphatase, and alpha-naphthyl esterase. Both ADH and papaverine produced a significant inhibition of renal damage. The subjective findings were quantitatively confirmed by measurement of enzyme activity, using the microscope photometer, and by morphometric studies with the Leitz-Classimat (determination on the basis of the alkaline phosphatase reaction) of the surface percentage of brush border in the proximal tubules. A disturbance of the hairpin counter-current system is to be considered as the cause of the renal lesion. This disturbance is caused by hydroxyquinoline-induced impairment of Na+/K+ transport, especially in the thick ascending limb of Henle's loop. Our results show that the hydroxyquinoline nephropathy can be favourably influenced both by stimulation of water re-absorption and possibly also transepithelial Na+ transport (ADH), and by increasing the blood flow of the arteriolae rectae with a resultant lowering of the intratubular urine concentration (papaverine). The dependency of hydroxyquinoline nephropathy on the phylogenetically determined concentration capacity of the kidney, and the effective influencing of the condition by ADH and papaverine indicate the importance of the degree of efficiency of the medullary countercurrent system in the pathogenesis of this renal lesion.
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PMID:The significance of the hairpin counter-current principle in the pathogenesis of toxic kidney lesions. An investigation of the influence of antidiuretic hormone and papaverine on hydroxyquinoline nephropathy of the rat. 88 59

Resistance to Marek's disease (MD) was selected over five generations in three strains and their four substrains. 2. MD mortality decreased consistently on natural exposure, but in birds artificially inoculated the mortality increased again in later generations. 3. Changes of gene frequencies at five polymorphic loci--blood group A, B and D, plasma esterase and alkaline phosphatase loci--were examined. 4. The frequencies of blood group A6 and B8 alleles showed a consistent trend of increase and the B11 allele gradually decreased in most strains. The Akp allele of alkaline phosphatase increased significantly in two of seven strains. 5. Comparisons of homozygotes and heterozygotes revealed a significant difference only at the A locus.
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PMID:Changes in polymorphic gene frequencies in strains of chickens selected for resistance to Marek's disease. 89 May 15

Human erythrocyte ghosts were solubilized in a low ionic strength medium containing 1% Triton X-100 and subjected to electrophoresis in polyacrylamide gels containing Triton X-100. Five major bands were stained with Coomassie Blue, all except one band being heterogenous when re-electrophoresed in gels containing sodium dodecyl sulphate. It was possible to detect acetylcholinesterase, non-specific esterase, ATPase, alkaline phosphatase, 5'-nucleotidase, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase, and aldolase activities on the Triton-containing polyacrylamide gels. Two of the enzymes, ATPase and 5'-nucleotidase, showed substantial inhibition by Triton X-100 in quantitative studies. This appears to be a useful method for studying membrane enzymes in normal and pathological red cells.
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PMID:Polyacrylamide gel electrophoresis of human erythrocyte membrane enzymes solubilized with triton X-100. 89 Sep 65

Polymorphism for the number of the blood proteins (transferrin, alkaline phosphatase, haptoglobin, plasma esterase and erythrocyte esterase) have been exposed by means of starch gel electrophoresis of northern fur seal Callorhinus ursinus from three regions: Pelagic of Commander Islands (n = 164), Rockery of Robben Island (Sakhalin population, n = 308), winter todder region to the east of Honshu Island (n = 110). The reliable differences have been exposed between fur seals of the Commander and Sakhalin populations by the gene frequencies Tf and Es pl. Fur seals from winter todder region turned out to be closer to Commander population, that to the Robben one. However, originality of gene frequencies of locus Ap (alkaline phosphatase), revealed from the todder region animals, is difficult to explain by the mixture of the Commander and Robben population only. Perhaps, this originality is caused by the influence of Pribylov population. It is established that hemoglobin of northern fur seal consists of two components (Hb I + Hb II). Individual variability of hemoglobin results in the variation of the colour intensity of the "slow" component (Hb II).
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PMID:[Biochemical polymorphism in the northern fur seal, Callorhinus usinus, from 3 regions of the northwestern part of the Pacific Ocean]. 90 85

The localization and enzymatic activity of alkaline phosphatase, non-specific esterase and leucine aminopeptidase were studied in normal menopausal corpus uterine tissues and its benign (fibromyoma and cellular fibromyoma) and malignant tumours (endometrium adenocarcinomata and spindle cell sarcoma). Alkaline phosphatase was localized in the cytoplasm and nuclear structures and showed the highest intense activity in well-differentiated papillary adenocarcinoma. Non-specific esterase was confined to the cytoplasm and was particularly marked in undifferentiated adenocarcinoma cells. Leucine aminopeptidase was considerably higher in spindle cell sarcoma than endometrium adenocarcinoma. Generally, the aforementioned enzymes were increased in neoplastic tumour tissues of the uterus than the homologous normal tissues.
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PMID:Cytoenzymology of benign and malignant tumours of the corpus uteri. III. Alkaline phosphatase, non-specific esterase and leucine aminopeptidase. 91 9

Using cytoenzymatic methods the activity of acid and alkaline phosphatase, non-specific esterase and glycogen content in peripheral blood cells were analyzed. A statistically significant rise in the activity of these enzymes was demonstrated in granulocytes and lymphocytes and a low level of glycogen in lymphocytes. Following prednisone treatment a statistically significant decrease was observed in the activity of studied enzymes and a rise in glycogen content.
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PMID:Cytochemical studies of peripheral blood granulocytes and lymphocytes in patients with systemic lupus erythematosus. 95 49


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