Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A nonisotopic enzymolysis assay method of phosphatidylinositol kinase (PIK) has been developed. Phosphatidylinositol 4-phosphate (PIP), phosphorylated from phosphatidylinositol (PI) by PIK was hydrolyzed with phosphainositide-specific phospholipase C. The product, inositol 1, 4-bisphosphate (IP2), was separated from inositol 1-phosphate (IP) with Dowex-1 column chromatography. Then, the IP2 was hydrolyzed by alkaline phosphatase to yield PI, and the PI was determined by colorimetry. The PIK activity was defined as PI nmol/mg protein per min. The recovery was 91%, CV = 6.5%
Hua Xi Yi Ke Da Xue Xue Bao 1991 Mar
PMID:[A nonisotopic enzymolysis assay method of phosphatidylinositol kinase]. 166 83

In this cross-sectional study, the radius bone mineral content (BMC) of 340 postmenopausal women (mean age: 53; mean years since menopause: 5.56) were assessed by single photon absorptiometry (SPA) for determining prevalence rate of postmenopausal osteoporosis in Chengdu, and some factors relative to BMC were investigated. Eighty-five of 340 postmenopausal women were diagnosed osteoporosis by SPA. The prevalence rate, which increases with year since menopause and age, is 25%. There is significant increase in prevalence after the age of 60 years or 5 years since menopause. Sixteen factors were analysed by STEPWISE REGRESSION. The variables selected were serum calcium(SCa), serum alkaline phosphatase (SAkP), urine calcium (UCa), urine hydroxypoline (UH), age, and Para. Fisher DISCRIMINANT ANALYSIS was used for diagnosis with these variables, the accuracy of diagnosis being 75.2%. Our study showed that postmenopausal osteoporosis is a common disease in Chengdu. High SCa, UH, age and low SAkP level may be risk factors of osteoporosis.
Hua Xi Yi Ke Da Xue Xue Bao 1991 Mar
PMID:[Study on morbidity and relative factors of postmenopausal osteoporosis in 340 women in Chengdu]. 177 38

Prior studies identified a cell-surface antigen, p75/150, that exclusively associated with the tumorigenic phenotype of the HeLa parent and the tumorigenic phenotype of the HeLa parent and the tumorigenic segregants of suppressed, nontumorigenic HeLa x human fibroblast cell hybrids. Candidate p75/150 cDNA clones were isolated from a D98/AH.2 (HeLa) cDNA library using oligonucleotide probes derived from p75/150 partial peptide sequence data. A data base search revealed close similarity of p75/150 with intestinal alkaline phosphatase (IAP) [Berger, J., Garantini, E., Hua, J. C. & Udenfriend, S. (1987) Proc. Natl. Acad. Sci. USA 84, 695-698]. We demonstrate that p75/150 is identical to HeLa IAP by the following criteria: (i) 47/49 amino acid identity of p75 peptide sequence with IAP, (ii) restriction maps for the p75/150 candidate cDNA clone and IAP are identical, (iii) partial DNA sequence analysis of p75/150 candidate cDNA clones revealed complete nucleotide identity with IAP, except for a single nucleotide substitution in the 5' untranslated region, (iv) transfection of a p75/150 cDNA expression vector into the nontumorigenic hybrid, CGL1, yielded p75/150 antibody-positive transfectants that also expressed partially heat-resistant alkaline phosphatase activity. Northern blot analysis demonstrated that high levels of HeLa IAP mRNA were expressed in D98/AH.2 and the tumorigenic segregant CGL4; however, no mRNA was detected in CGL1. Nuclear run-on analyses indicate that HeLa IAP mRNA expression in the HeLa x fibroblast hybrids is regulated at the level of transcription initiation. Furthermore, evidence is discussed supporting the involvement of a chromosome 11 tumor suppressor locus in the regulation of HeLa IAP gene expression.
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PMID:Identification of the HeLa tumor-associated antigen, p75/150, as intestinal alkaline phosphatase and evidence for its transcriptional regulation. 230 98

This study aimed to observe the influence of the new compound-XW630 on the proliferation of osteoblast, ALP action, and the forming of osteoclast. The MTT method and the alkaline phosphatase method were adopted to investigate the influence of XW630 on ALP action and the proliferation of osteoblast cultured from grown rat's skull. The Tartrate-resistant acid phosphatase dyeing method was used to observe the influence of XW630 on forming of cultured osteoclast in vitro. The result showed that, when XW630 concentration was 10(-6)-10(-8) mol/L, it obviously enhanced the proliferation of osteoblast and improved the activity of ALP, and it evidently provented PTH from stimulating the forming of osteoclast. It is concluded that XW630 is obviously effective for stimulating the proliferation of cultivated osteoblast in vitro and for inhibiting the forming of osteoclast.
Hua Xi Yi Ke Da Xue Xue Bao 1998 Dec
PMID:[Effects of the new compound-XW630 on osteoblast]. 1074 29

NF-IL6 (Nuclear factor for IL-6 expression) is involved in inflammatory reaction, expression of acute-phase proteins and cytokines, apoptosis and suppression of tumor cells, and maintenance of macrophage immunological functions. To investigate the role of highly expressed exogenous NF-IL6 in macrophage tumor cytotoxicity, a recombinant expression plasmid, pCN, which harbored the coding region of NF-IL6, was transfected into murine primary cultured peritoneal resident macrophages by an improved DEAE-dextran method. Western blot showed the high expression of NF-IL6 in these macrophages. Then the tumor cytotoxicity of the NF-IL6-overexpressing macrophages from normal and nude mice was measured by an alkaline phosphatase assay, using the human hepatocarcinoma cell line SMMC 7721 as target cells. Results showed that the overexpression of NF-IL6 enhanced the tumor cytotoxicity in both types of macrophages, demonstrating that the expression level of the NF-IL6 gene was directly related to the tumoricidal activity in these cells.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 2001
PMID:Enhancement of Macrophage Cytotoxicity by Overexpression of Exogenous NF-IL6 Gene. 1205 86

CD20 is a specific antigen expressed on normal and neoplastic B cells exclusively. Recent researches showed that in B cell leukemia, CD20 was over-expressed. Therefore monoclonal antibody (McAb) to CD20 may be of clinical value in diagnosis and treatment of some leukemias and lymphomas. In this study, the full length gene of CD20 cDNA were cloned from total RNA of Raji cells, inserted into an eukaryotic expression vector pcDNA3.1, forming a recombinant plasmid pcDNA3.1/CD20. NIH-3T3 cells were transfected with pcDNA3.1/CD20 and selected with G418 for the transfected cells. Alkaline phosphatase against alkaline phosphatase assay(APAAP) experiments showed that the selected cells could express the human CD20 onto its surface. Balb/c mice were immunized with CD20( ) NIH-3T3 cells once three weeks for 3 shuts. Indirect immunofluorescence experiments were done with the Raji cells and the serum of the immunized mice, and the results showed that the spleen of the immunized mice could be used to prepare the McAb to CD20.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 2000
PMID:Cloning and Expression of Human CD20 Gene on NIH-3T3 Cell Membrane. 1207 39

Human epiregulin cDNA was amplified from the lung cancer cell line A549 using RT-PCR. After adding 6 His codon to its 3' end, it was cloned into a high efficient secretive Escherichia coli system with alkaline phosphatase promoter(phoA promoter)constructed in our lab and induced for expression. The product was purified one-step by Ni-NTA column. Amino acid sequence analysis revealed the identity of our product with that previously reported. The product showed strong proliferative effect on fibroblast cell line Balb/c3T3 and growth inhibitory effect on epithelial carcinoma cell line A431.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 2000
PMID:Expression of Human Epiregulin in E.coli. 1207 58

Osteoblast-like cells were isolated from embryonic human calvarium with modified enzyme digestion method. TGF-beta which is believed having biological effects on bone cells was added into the culture medium with separate concentrations. The incorporation of 3H-TdR, 3H-Proline of osteoblast-like cells and alkaline phosphatase(ALP) content of the cells were tested to study the effects of the TGF-beta on the proliferation of osteoblasts. The results showed that TGF-beta increased ALP activity and the synthesis of collagen, and the cell proliferation was inhibited. It affected the proliferation and differentiation of osteoblast-like cells with the best 1 ng/ml among four concentration of 0.01-10 ng/ml. These findings suggested that function of TGF-beta might be modified by other local growth factors.
Hua Xi Kou Qiang Yi Xue Za Zhi 1998 Feb
PMID:[The effects of TGF-beta on the proliferation and differentiation of human osteoblast-like cells]. 1207 91

An artificial affinity ligand comprising a p-aminobenzyl phosphoric acid as inhibitor was designed and coupled to an agarose support by chlorotriazine ring to separate and purify alkaline phosphatase from calf intestine. The optimal density of the ligand is 6 &mgr;mol/g adsorbent. After elution with sodium chloride and inorganic phosphoric acid a 300-fold purified enzymatic preparation was obtained in one step with a yield of 90%.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 1998
PMID:Application of a Designed Synthetic Ligand for the Separation and Purification of Alkaline Phosphatase. 1217 54

This study aimed to identify the behaviour of mandibular condylar chondrocytes in vitro. Cells were harvested from the mandibular condyles of 3 week-old S. D. rats. Cell structure, morphology and characteristics were assessed by phase-contrast microscopy, scanning electron microscopy, enzymohistochemistry and immunohistochemistry. The cultured condylar chondrocytes, stellated or spindle-shaped, could grow in several layers and form many cell colonies. They could secret proteoglycans, alkaline phosphatase, type II collagen, et al. The methods of isolation, culture and identification of condylar chondrocytes were presented and discussed, which can be adopted in probing further into the cellular mechanism of functional orthopedics.
Hua Xi Yi Ke Da Xue Xue Bao 1999 Mar
PMID:[The behaviour of rat mandibular condylar cartilage in cell culture]. 1220 13


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