Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A trial was performed in 204 healthy calves (heifers) of the Bohemian Spotted breed in the post-natal period from birth to the age of four months. The activities of the following enzymes in blood plasma were determined: L-aspartate: 2-oxoglutarate: aminotransferase, EC.2.6.1.1. (GOT), L-alanine: oxoglutarate: aminotransferase, EC.2.6.1.2. (GPT), L-lactate: NAD oxidoreductase, EC.1.1.1.27 (LDH), and orthophosphoric acid monoester phosphohydrolase, EC.3.1.3.1. (alkaline phosphatase). The calves were divided into age categories according to the date of birth with an interval of one week. GOT activity in blood plasma increased significantly until the age of eight weeks (from the original value of 1.1708 +/- 0.2598 micronmol ml-1 to 1.8150 +/- 0.6362 micronmol ml-1, with the maximum of 2.0317 +/- 0.7777 micronmol ml-1 of plasma in the sixth week). In the subsequent period the GOT curve has not a characteristic course. While the activity of GOT increased in the first weeks after birth, the activity of GPT showed a significant drop (from the original level of 0.9000 +/- 0.3364 micronmol ml-1 to the minimum of 0.3675 +/- 0.1901 micronmol ml-1 of plasma in the seventh week); from the 10th week on the values rise so that at the end of the period of study they reach almost the same levels as in calves in the first postnatal week. The activity of LDH in blood plasma remains at almost the same level in the first five weeks after birth (between 43.4025 +/- 8.4893 micronmol ml-1 and 46.3792 +/- 14.8952 micronmol ml-1 of plasma); it was at a statistically significantly higher level only in a short period between the 7th and 10th week after birth. The highest values of alkaline phosphatase in blood plasma were recorded at the age of two or three weeks (maximum in the second week 23.9833 +/- 9.0945 micronmol ml-1 of plasma); from the fourth week on, the values of alkaline phosphatase are significantly lower until the end of the test period, ranging betweek 5.3133 +/- 1.6017 micronmol ml-1 and 7.5425 +/- 2.2437 micronmol ml-1 of plasma. Changes conditioned by postnatal development were observed in the development of all the enzymatic activities under study, the greatest changes being observed in alkaline phosphatase.
 ...
PMID:[The development of transaminase activity (SGOT and SGPT), lactate dehydrogenase and alkaline phosphatase in the blood plasma of calves up to the age of 4 months]. 82 94

1. We have studied the in vitro effect of 39 drugs on 17 biochemical parameters determined by a SMAC System. Only two drugs were found to interfere: ascorbic and theophyline. 2. The ascorbic acid lowers the glucose and the bilirubine values; it increases the creatinine and the uric acid concentration. At concentration smaller than 5 mg/dl of this drug, these effects are negligible. 3. We have found a new drug interference: theophylline inhibits the alkaline phosphatase and LDH activities. This effect is not negligible on alkaline phosphatase for therapeutic levels of this drug; the action on LDH can be ignored at normal therapeutic range. 4. For a given drug, we have found different interference with biochemical parameters determined with various commercial lyophlised control sera or a liquid pool of sera. This indicates that the type of sera used in drug interference studies must be described.
 ...
PMID:The in vitro effect of drugs on biochemical parameters determined by a SMAC system. 83 28

Preoperative LDH, CPK, alkaline phosphatase and liver scan results were compared with the presence or absence of hepatic metastases at operation in 124 laparotomies in 123 patients with colorectal carcinomas during a three-year period. The overall accurcy rates for the four tests ranged from 74 to 84 per cent. The false-negative rates were acceptably low (9-13 per cent) for all but the CPK determination. The false-positive rates were unacceptably high for all four tests (38 to 60 per cent). A 'metastatic score' for reducing the high false-positive rate is described. In view of the high false-positive rates of preoperative screening tests, laparotomy is presently the most accurate method of diagnosing hepatic metastases.
 ...
PMID:The preoperative diagnosis of hepatic metastases in cases of colorectal carcinoma. 84 94

50 guinea pigs were allergized three times in 3 days intervals by subcutaneous injection of 25% solution of egg-white in physiological saline in a dose of 0-1 ml/100 g of body weight. On the 21 day after the last injection the animals were exposed to aerosol of antigen of egg-white. 11 animals died in acute anaphylactic shock. The control group consisted of 12 guinea pigs which received subcutaneously a solution of physiological sodium chloride of the same dosis--0-1 ml/100 g of body weight and were also exposed to the allergen, together with the experimental group. The removed parathyroids together with the thyroid gland were studied with histologic and cytoenzymatic methods. The activity of alkaline and acid phosphomonoesterase, nonspecific AS-naphtol acetate esterase, succinic (SDH), lactic (LDH) and D-L-alfaglicerophosphate dehydrogenase (alpha-GPD) were tested. No morphological changes in the parathyroids of guinea pigs in anaphylactic shock were found. Instead a decrease of the enzymatic activity of dehydrogenases was found, what might be connected with the decrease of metabolic activity of the cells. The decrease of alkaline phosphatase activity in the endothelial cells of the capillaries was another finding. It is likely that in an acute anaphylactic shock in guinea pigs only functional changes develop which have not counterparts in histology visible under the light microscope.
 ...
PMID:Cytoenzymatic investigations of parathyroid glands in acute anaphylactic shock of guinea pig. 86 38

Ten subjects were exposed to high-G on the human centrifuge using seatback angles of 13 degrees, 30 degrees, 45 degrees, 60 degrees, and 75 degrees from the vertical, and body configurations of the lower portion of the body with pelvis and legs elevated, pelvis, elevated, and pelvis elevated with knees on chest (fetal position). Tolerance was measured by peripheral light loss. Mental status, respirations, core and ambient temperatures, and ECG were monitored. Daily physio-chemical data included: creatinine, bilirubin, phosphorus, alkaline phosphatase, uric acid, cholesterol, total protein, albumin, BUN, glucose, LDH cardiac isoenzyme No. 5, SGOT, SGPT, CPK, CBC, and urinalysis. Tiredness, pressure on the chest, and general discomfort in the fetal position were reported. Physical examination demonstrated petechiae. Heart rate, respiratory rate, and temperature increased post-session. There was a significant rise in values for albumin, chloride ion, creatinine, calcium, LDH, BUN, and immature white cells; and a decrease in values for phosphorus, SGOT, SGPT, protein, uric acid CO2, globulin, hematocrit, monocytes, and eosinophils.
 ...
PMID:Psycho-physiological assessment of acceleration-induced changes in various seat configurations. 86 40

Gastric and duodenal ulcers were characterized by the high activity of alkaline phosphatase within the zone of impairment. In gastric ulcer the high concentration of the enzyme was observed also in the region of minor curvature. Activities of aspartate and alanine transaminases were decreased in the zone of gastric ulcer; in duodenal ulcer only the alanine transaminase activity was decreased. The total lactate dehydrogenase activity was unaltered in gastric ulcer, but it was increased in impaired duodenum, where, among the other LDH isozymes, LDH5 fraction was increased.
 ...
PMID:[Non-proteolytic enzymes of human gastric and duodenal mucosa in peptic ulcer]. 103 Aug 92

The influence of Uden, exerted on the activity of GOT, GPT, LDH acid phosphatase and alkaline phosphatase in the serum, is investigated upon 4-day-long treatment. The experiment is conducted in three groups of white male rats with mean weight 270 +/- 20 g. The first group of animals underwnt poisoning with 1/20 LD50, the second--with 1/4 LD50 while in the third, control group, the animals were treated with an oil solvent at the respective days. It is found that acid phosphatase, GOT and alkaline phosphatase activity in group two is virtually unchanged, whilst GPT activity in both experimental groups, and acid phosphatase in group one are reliably increased (P less than 0,01), with 20,17 and 53 per cent respectively. LDH activity is reliably lowered with 60 per cent in group two, whereas in group one a tendency for a reduction is noted (P less than 0,05).
 ...
PMID:[Changes in enzymatic activity in rats with acute Unden poisoning]. 103 21

Blood samples from rats, rabbits and beagles were subjected to simultaneous analyses for ten parameters using the RaBA-System and the results were compared with those obtained by the routine manual methods. Although the mean values measured by the two methods differ in several items, a significant correlation between the two methods in the majority of the determinations makes the use of the RaBA-System acceptable. The RaBA-System is thus considered to be applicable to evaluation of hemoglobin, total protein, glucose, BUN, total cholesterol, GOT, GPT, and alkaline phosphatase in blood samples from rats, rabbits and beagles. Plasma LDH and albumin, however, cannot be determined with accuracy by the system.
 ...
PMID:[Application of the RaBA-System to clinical blood chemistry test in experimental animals]. 103 65

ASSAY CURVES, USING A DISK DIFFUSION METHOD FOR THE ANTIBIOTICS GENTAMICIN AND CEFAZOLIN, WERE PREPARED WITH: saline, saline plus 10% serum, and ascitic, synovial, cerebrospinal, and pleural fluids. The curves were compared with a standard curve prepared with pooled human serum. The pH, total protein, glucose, blood urea nitrogen, sodium, potassium, calcium, phosphorus, chloride, CO(2) content, uric acid, cholesterol, bilirubin, serum glutamic oxalacetic transaminase, CPK, LDH, and alkaline phosphatase were determined and compared for all fluids. Measurements for cefazolin levels were falsely elevated in those fluids with low protein content when serum was used as a reference standard. There was a linear inverse relationship between the protein content of the fluids and the cefazolin level with serum as the standard for the assay of this highly protein-bound antibiotic. No discrepancies were observed in the assay curves for gentamicin, an antibiotic known not to be bound by serum proteins.
 ...
PMID:Effect of protein concentration and binding on antibiotic assays. 109 4

Thirty-two conditioned 15-18 kg dogs underwent laparotomy, heparinization, left lateral hepatic lobe injury and subsequent partial left lateral hepatic lobectomy. The hemostatic capabilities of the surgical scalpel combined with suture ligatures and stay sutures, the Bovie, and the CO2 laser were compared. The CO2 laser proved significantly more effective in achieving hemostasis both in comparison to the Bovine and the surgical scalpel combined with classical methods. The postoperative mortality utilizing the Bovie was 11%, the scalpel 30% and the laser 23%. Two of the three laser deaths were related to a bulky laser delivery system which can be redesigned. Postoperative laboratory evaluation revealed that SGOT, LDH, alkaline phosphatase, total protein, albumin and hemoglobin levels were altered in the postoperative period but returned to normal levels in uniform fashion in all groups. There was no statistical difference between various surgical modalities with regard to these parameters. The white blood count was significantly lower in laser dogs when compared to the other two groups. Other laboratory parameters were unchanged. Damage to liver tissue may be less extensive when the laser is utilized as opposed to the Bovie or stay suture methods of hemostasis and healing is equally good. The CO-2 laser is considered a valuable ancillary tool in hepatic resection and clinical evaluation is warranted.
 ...
PMID:Laser surgery in exsanguinating liver injury. 111 70


<< Previous 1 2 3 4 5 6 7 8 9 10 Next >>