Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Bronchoalveolar lavage fluid neutrophils were studied by the cytologic methods in 58 patients with chronic bronchitis, 63 ones with bronchiectasis, and 8 normal controls. The study included cytospectrophotometry of myeloperoxidase and alkaline phosphatase activity and estimation of active oxygen-producing cells in the NBT test. Neutrophilic functional activity was different in the patients with chronic bronchitis and bronchiectasis. Neutrophilic myeloperoxidase and alkaline phosphatase activities were lower in the patients with chronic bronchitis than in those with bronchiectasis, whereas the counts of cells active in the NBT test were low in both patient populations.
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PMID:[Functional activity of the neutrophils in the bronchoalveolar space in chronic bronchitis and bronchiectasis]. 171 28

A number of cytochemical characteristics and the NBT test were studied in neutrophilic granulocytes of 194 patients with diffuse pulmonary carcinoma (Stages III-IV), 31 patients with chronic nonspecific pulmonary diseases, and 20 normal subjects. Changes in the neutrophilic morphology and function were revealed in lung cancer patients, presenting as elevated alkaline phosphatase activity, reduced myeloperoxidase activity and lipid and glycogen levels, increased endogenous activation of the neutrophils in the NBT test, and decreased reaction activity in zymosan stimulation. Antitumor chemotherapy involved a lowering of the cationic protein level, as well of the acid phosphatase activity, and elevation of glycogen content. Stimulated NBT test was highly sensitive to cytostatic therapy. Tumor dissemination and morphologic variant contributed to changes in the neutrophilic morphology and function.
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PMID:[The cytochemical characteristics of neutrophil leukocytes in lung cancer before and during antineoplastic chemotherapy]. 172 28

Colostrum cell composition observed during the first 24 hrs following delivery objectively reflects the degree of prelactation preparation of the breast. The state of neutrophilic leucocytes appeared to be the most informative in this respect. In normal lactation, maximal number of neutrophilic leucocytes was found during the first 24 hrs after delivery. The leucocyte inflow to the colostrum is highly increased in hypogalactia. Leucocytes aggregation which is the most characteristic for normal lactation did not occur in hypogalactia. The activity of alkaline phosphatase and myeloperoxidase of leucocytes is much higher in normal lactation and much lower in hypogalactia. The evaluation of alkaline phosphatase activity and sudanophilic lipids content shows no differences related to the lactation level.
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PMID:[A physiological validation for the early diagnosis of hypogalactia in women]. 172 69

It was established that acrylonitrile (AN) toxic action resulted in changes in the morphofunctional characteristics of white blood cells. Deviations were found in the activity of the marker enzymes of alkaline phosphatase neutrophil secretory granules and myeloperoxidase. Other changes were established in the markers of acid phosphatase lysosomes, the enzyme of plasma membrane--5 nucleotidase, in the endoplasm network of glucose-6-phosphatase, in succinate dehydrogenase mitochondria, and in alpha-glycerolphosphate dehydrogenase. Along with a considerably lowered succinate dehydrogenase activity, lymphocyte cell deformation against the activity of this enzyme was detected. The data obtained specified newly revealed changes in the blood at AN intoxication and indicated to innovated means of its prophylaxis.
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PMID:[Study of the activity of membrane-bound leukocyte enzymes in chronic acrylonitrile poisoning in experimental studies and occupational environment]. 196 27

Pneumonic pasteurellosis was experimentally induced in calves by inoculation of 5 x 10(8) Pasteurella haemolytica organisms into the right diaphragmatic lung lobe. Blood and bronchoalveolar lavage fluid samples were obtained prior to inoculation and at postinoculation hour (PIH) 2, 4, and 6. Calves developed acute lung injury, characteristic of pneumonic pasteurellosis. Lesions were found only in the right diaphragmatic lobe. By PIH 4, significant (P less than 0.01) increases were detected in lavage fluid total cell count, neutrophil count, total protein and albumin concentrations, and alkaline phosphatase (ALP) and lactic dehydrogenase (LD) activities. Myeloperoxidase and elastase activities did not increase. Neutrophil depletion ameliorated the lung lesions and prevented the increase in lavage fluid cell count, total protein, and albumin concentrations and ALP and LD activities. Treatment with the iron chelator, deferoxamine mesylatehydroxyethyl starch, attenuated the increase in total protein and albumin concentrations and ALP and LD activities at PID 4, but not PIH 6. Treatment with a neutrophil function inhibitor, pentoxifylline, prevented the increase in lavage fluid neutrophil numbers, but accentuated the increase in total protein and albumin concentrations, and ALP, LD, myeloperoxidase, and elastase activities.
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PMID:Changes in blood and bronchoalveolar lavage fluid components in calves with experimentally induced pneumonic pasteurellosis. 201 47

We report two cases of Philadelphia (Ph1) chromosome positive acute mixed lineage leukemia (AMLL) with breakpoint cluster region (bcr) (M-BCR-1) rearrangement. A 31 year-old-man (case 1) and a 42 year-old-woman (case 2) were admitted to our hospital for further evaluation of leucocytosis with atypical blasts. Each case was diagnosed as having bilineal type of AMLL because: (1) blasts in each case consisted of larger myeloid cells positive for myeloperoxidase and small lymphoid cells positive for PAS, and blasts in case 2 were positive for TdT; (2) blasts in case 1 expressed B lymphoid associated antigen; (3) Southern analysis in each case showed clonal rearrangements of both the immunoglobulin heavy chain and the T cell receptor beta gene. These two cases demonstrated the Ph1 chromosome and rearrangement of the bcr (M-BCR-1) gene, but none of splenomegaly, basophilia, and additional chromosome abnormalities were observed. In addition, after achieving remissions, they didn't revert to chronic phase of chronic myelogenous leukemia (CML) and showed normal neutrophil alkaline phosphatase scores, and the Ph1 chromosome disappeared completely in case 1 and coexisted with the normal chromosome in case 2. These findings suggest that diagnosis of both cases should not be CML blast crisis (BC) but Ph1 positive acute leukemia, and Ph1 positive AMLL may be a distinct clinical entity to be distinguished from CML-BC.
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PMID:[Philadelphia chromosome positive acute mixed lineage leukemia with bcr (M-BCR-1) rearrangement]. 215 95

This communication presents four different assay systems for the determination of myeloperoxidase in body fluids. One is based on conventional chemiluminescence, two on luminescence-amplified enzyme measurement using either spiroadamantane-1,2-dioxetanes with alkaline phosphatase or luminol/peroxidase/4-iodophenol coupled with a peroxidase label. The assays covered the range 0-600 micrograms/l peroxidase. Established reference range for EDTA plasma from healthy volunteers gave an upper limit of 250 micrograms/l (95% confidence limits). Intra-assay coefficients of variation were less than 5% for all assays, as seen in compound precision profiles. Inter-assay variation was less than 10% throughout the whole concentration range. Kinetic curves for the light production were performed over a 2 hour period for the enhanced luminescence assays. Dynamic ranges of these assays were compared with the conventional colorimetric assay using 4-nitrophenyl phosphate--alkaline phosphatase. The assay was standardized using a commercially available myeloperoxidase preparation with defined enzymatic activity. The protein content was estimated and the laboratory standard compared and calibrated in mass units.
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PMID:A comparison of four immunometric assays for myeloperoxidase using luminescent and colorimetric signal detection. 217 39

This short communication describes the use of spiroadamantane-1,2 dioxetane substrates for alkaline phosphatase and beta-D-galactosidase in the development of luminescence-enhanced enzyme immunoassays. Until the present work, only peroxidase-luminol/peroxidase systems had been used in such assays. The light reaction kinetics were studied from the time of initiation of the light reaction up to 2 days later. The methods is illustrated by the determination of myeloperoxidase, based on microtitre plate technology, coupled with a microtitre plate luminometer which needs no injector. The alkaline phosphatase and peroxidase systems were compared.
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PMID:Spiroadamantane dioxetane substrates--stable labels for luminescence-enhanced enzyme immunoassays. 223 Jun 66

Alkaline phosphatase and myeloperoxidase was examined in patients with chronic ischemic heart disease and myocardial infarction. It was established that patients with myocardial infarction showed high values of alkaline phosphatase while the values of myeloperoxidase were low. Dynamic evaluation of alkaline phosphatase and myeloperoxidase of leucocytes allows to assess the course of myocardial infarction and efficacy of its treatment.
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PMID:[Granulocyte alkaline phosphatase and myeloperoxidase in patients with ischemic heart disease]. 254 22

In an attempt to assess the effect of alcohol per se on human polymorphonuclear neutrophils (PMN), irrespective of other physiopathological parameters, we examined neutrophil function in healthy volunteers who had taken a single large dose of whisky. Before and at different times after ingestion, several PMN properties were simultaneously tested including random migration, in vitro chemotaxis, adherence, aggregation, cytochrome C reduction, phagocytosis, bacterial killing, intracellular cAMP and cGMP contents, myeloperoxidase, and neutrophil alkaline phosphatase scores. Only phagocytosis of Staphylococcus aureus was significantly depressed after alcohol ingestion. Adherence was inhibited only in some individuals when their respective blood alcohol levels were the highest. Both alterations were moderate and reversible. These data point out the limited effect of occasional alcohol consumption on the different facets of neutrophil behavior. These findings suggest that factors other than alcohol itself could be concerned in the marked PMN dysfunction well established in chronic alcoholism.
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PMID:Human neutrophils are not severely injured in conditions mimicking social drinking. 255 61


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