Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Electrical or mechanical stimulation has been applied widely in bone fracture treatment or bone tissue construction. The effect of combined mechanical and electrical stimulation to osteoblasts or bone tissue has not been deeply studied, which would provide a more actual simulation of in vivo microenvironment. In this study, a cell culture device that can simultaneously load mechanical and electrical stimulation on cells was self-designed and fabricated. The combined laminar shear stress and electrical stimulation could be supplied to MC3T3-E1 cells seeded on the nano-crystalline fluorine-doped tin oxide glass plate. Cell counting kit-8 assay was used to study the proliferation of MC3T3-E1 cells. The changes in alkaline phosphatase activities, genes expression of collagen-I (Col-I) and fibronectin and concentration of Col-I were also measured. Compared with single stimulation, the combined stimulation obviously enhanced proliferation, differentiation and synthesis of extracellular matrix of pre-osteoblasts in a greater extent. The combined mechanical and electrical stimulation provide a new way to promote in vitro cultivation of cells and tissues, which will be very significant in the development of tissue engineering.
 ...
PMID:Promoting Proliferation and Differentiation of Pre-Osteoblasts MC3T3-E1 Cells Under Combined Mechanical and Electrical Stimulation. 3089 Feb 24

There have been only a limited number of reports on primary adult T cell lymphoma/leukemia (ATL) in the bone. This is a case report of a 75-year-old patient initially reporting multiple bone pains that were attributed to osteolytic ATL. The patient developed spontaneous chest/back pain and visited a local hospital. Laboratory tests showed high levels of alkaline phosphatase (ALP), and computed tomography (CT) revealed skeletal lesions with osteolysis. Although multiple myeloma was initially suspected, the results of bone marrow aspiration and bone biopsy were inconsistent. After he was referred to our hospital, mild hypercalcemia (10.4 mg/dL) with low-normal intact parathyroid hormone (PTH) (27 pg/mL), low parathyroid hormone-related protein (PTHrP), and elevated 1,25-dihydroxy vitamin D (1,25OH2D) levels (136 pg/mL) narrowed the differential diagnosis down to lymphomatous and granulomatous diseases, and then, the high serum soluble IL-2 receptor (3,450 U/mL) and the flower cells recognized in the peripheral blood sample suggested the involvement of ATL. Finally, the reevaluation of the iliac bone biopsy sample led us to the histological diagnosis of ATL infiltration in the bone. The subsequent two courses of chemotherapy in addition to denosumab resulted in an objective partial metabolic response indicated in 18-fluorine-fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT). Although very rare, the bone involvement of ATL could be used for the differential diagnosis for local osteolytic bone pain in addition to multiple myeloma and metastatic bone diseases.
 ...
PMID:Primary bone adult T cell lymphoma with multiple skeletal lesions and debilitating painful osteolysis: a case report. 3312 74


<< Previous 1 2 3 4 5 6 7 8 9