Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound   Target Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound

---

Query: EC:3.1.3.1 (alkaline phosphatase)
47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Serum chemistry test results were correlated with discharge diagnoses of 4,295 persons admitted to an acute care general hospital in a seven-month period. The diagnostic value of the test results, separately and in combinations of up to 12 tests, were calculated at each of ten levels as measured from the mean of normal in standard deviations. All patients had a serum profile consisting of total calcium, inorganic phosphorus, glucose, urea nitrogen, uric acid, cholesterol, total protein, albumin, total bilirubin, alkaline phosphatase, lactic dehydrogenase, and glutamic oxaloacetic transaminase levels. Analysis by manual and computer technics is illustrated. Computerized analysis of a chemistry profile is reported as a list of diagnostic possibilities in ranking order, with the calculated probability for each. In this preliminary report individual diseases are replaced by categories of disease taken from The International Classification of Diseases, Adapted (ICDA).
...
PMID:Diagnostic value of a chemistry test profile. 101 35

Development of experimental chronic renal insufficiency in rats was accompanied by an increase in concentration of residual nitrogen and phosphorus in blood and also by a decrease in intestinal absorption of Ca2+, however, no hypocalcemia was observed and the alkaline phosphatase activity was unaltered in blood serum. At the same time the renal insufficiency caused in some animals metastatic calcification of aorta and kidney, which was manifested by increased calcium concentration in these tissues. Administration of dihydrotachysterol increased the active transport of Ca2+ in rat intestine at the later steps of the impairment and led to development of moderate hypercalcemia and particularly to an increase in the degree of calcinosis of aorta and kidney. Administration of thyrocalcitonine did not prevent the hypercalcemia and calcinosis of internal tissues.
...
PMID:[Changes in phosphorus-calcium metabolism in experimental renal insufficiency and after administration of dihydrotachysterol and thyrocalcitonin]. 103 Aug 98

ASSAY CURVES, USING A DISK DIFFUSION METHOD FOR THE ANTIBIOTICS GENTAMICIN AND CEFAZOLIN, WERE PREPARED WITH: saline, saline plus 10% serum, and ascitic, synovial, cerebrospinal, and pleural fluids. The curves were compared with a standard curve prepared with pooled human serum. The pH, total protein, glucose, blood urea nitrogen, sodium, potassium, calcium, phosphorus, chloride, CO(2) content, uric acid, cholesterol, bilirubin, serum glutamic oxalacetic transaminase, CPK, LDH, and alkaline phosphatase were determined and compared for all fluids. Measurements for cefazolin levels were falsely elevated in those fluids with low protein content when serum was used as a reference standard. There was a linear inverse relationship between the protein content of the fluids and the cefazolin level with serum as the standard for the assay of this highly protein-bound antibiotic. No discrepancies were observed in the assay curves for gentamicin, an antibiotic known not to be bound by serum proteins.
...
PMID:Effect of protein concentration and binding on antibiotic assays. 109 4

The effects of lynestrenol, administered continuously in the dose of .5 mg/day, were studied in 50 women during 1146 menstrual cycles. 45 patients completed 24 cycles each. Before treatment and every 6 cycles all patients were subjected to questioning, physical examination, cytological, blood and urea nitrogen tests and urinalysis. 50% were also subjected to hepatic function tests (sulfobromophtalein excretion, glutamic oxalacetic transaminase, alkaline phosphatase, cephalin-cholesterol flocculation) and endometrial biopsies every 6 cycles. Subjective side effects were absent and weight and blood pressure remained unchanged. Laboratory tests and cytological studies showed normal results. Only 14% of the biopsies performed showed interferen ce with the normal hormonal transformation of the endometrium. The average duration of menstruation was 4-5 days; 7% of all cycles were shortened by 5-10 days and .2% by 11-14 days. 7% of the cycles were prolonged by 5-10 days and .8% by 11-20 days. Amenorrhea was present in .4% of the cycles. Irregular bleeding (either spotting or breakthrough bleeding) was present in 14% of the cycles. 6 patients (12%) stopped the treatment for this reason. It is concluded that the preparation studied is effective and very well tolerated. Patients must be advised regarding the possibility of irregularities in the menstrual cycle, the efficacy of the drug and the absence of other unwanted side effects, to decrease the dropout rate.
...
PMID:[Effects of lynestrenol used singly in low doses, as a contraceptive]. 110 Apr 81

An enzyme capable of hydrolyzing the substrate L-alanine p-nitroanilide has been found in the various Escherichia coli strains tested. This enzyme has been called aminoendopeptidase since it shows both activities (see accompanying paper). It is released from the cells by osmotic shock and by lysozyme -- EDTA spheroplasting treatment, and 50% of the total activity is directly detectable with suspensions of intact cells. However, the release by osmotic shock or spheroplasting is not as efficient as it is for alkaline phosphatase. This periplasmic aminoendopeptidase is constitutively produced but the differential rate of synthesis is increased 4-fold when the cell growth is limited by Pi. The occurrence of this 'derepression' is simultaneous with that of alkaline phosphatase. Increasing the concentration of inorganic phosphate in the medium has no effect on the constitutive aminoendopeptidase synthesis. The effect of phosphate starvation is specific since starvation for neither nitrogen nor carbon and energy source are effective in derepressing aminoendopeptidase.
...
PMID:Evidence for an aminoendopeptidase localized near the cell surface of Escherichia coli. Regulation of synthesis by inorganic phosphate. 110 39

Ultramicro procedures requiring 5-10 mul of serum or blood per analysis were used in determining blood constituents of healthy full-term newborns during the first four days of life. The resulting values appeared to be influenced by age, sex, and race. Values for total protein, albumin, urea nitrogen, and uric acid in serum decreased with time; serum inorganic phosphorus and whole-blood aldosaccharoses increased. Serum from females had higher values than that from males for total proteins, albumin, and inorganic phosphorus. The values for serum calcium and alkaline phosphatase were consistently higher in Negro than in white infants; values for uric acid were higher in the latter.
...
PMID:Ultramicroscale determination of clinical chemical values for blood during the first four days of postnatal life. 112 19

Twenty patients with generalized symptomatic Paget's disease had serial measurements of radiocalcium turnover and/or total body elemental composition by in vivo neutron activation analysis during long-term calcitonin therapy. Despite maintained clinical improvement, seven of 15 patients showed partial or total loss of the initial decelerating effect of calcitonin on skeletal turnover, whereas the remaining eight patients maintained the calcitonin-induced deceleration. The changes in skeletal turnover were roughly proportional to the induced changes in serum alkaline phosphatase and urinary hydroxyproline. However, disparities in the magnitude of the changes among the three parameters were not uncommon. Total body calcium was increased by a mean of 22% above predicted prior to calcitonin and decreased significantly by 4% during long-term calcitonin treatment. Total body phosphorus, nitrogen, and sodium also decreased. The phosphorus and sodium losses appeared to be mostly from the skeleton. These data confirm histologic evidence of the disappearance of pagetic bone, resumption of normal lamelar bone formation, and radiographic evidence of a decrease in bone volume during calcitonin treatment and incidate the relative magnitude of this effect. The action of calcitonin in this regard possibly represents a specific effect on Paget's disease beyond its general skeletal effect of reduce cellular activity.
...
PMID:Skeletal turnover and total body elemental composition during extended calcitonin treatment of Paget's disease. 112 38

The development of toxicity to 4'-demethylepipodophyllotoxin-9-(4,6,-O-thenylidene-beta-glucopyranoside) an epipodophyllotoxin with oncolytic activity, was characterized in mice treated three times at 3-day intervals with 10 mg of drug i.p. per kg of body weight. Changes in organ function and general metabolism were determined by measuring 18 constituents of blood for up to 10 weeks after drug administration. The results indicate three distinct phases of toxicity to 4'-demethylepipodophyllotoxin 9-(4,6-O-2-thenylidene-beta-glucopyranoside). Acute toxicity developed within the first 10 days and was expressed by a depressed hematocrit and elevated plasma levels of glutamate-pyruvate transaminase, glutamate-oxaloacetate transaminase, lactic dehydrogenase, amylase, lipase, and uric acid. By 4 weeks, levels ahd returned to normal. The acute phase was followed by a chronic phase, which was characterized by progressive decreases in plasma levels of glucose, cholesterol, albumin, and total protein. Finally, about 7 weeks after treatment, a terminal phase indicated by correlated increases in glutamate-pyruvate transaminase, glutamate-oxaloacetate transaminase, lactic dehydrogenase, and blood urea nitrogen became apparent. Plasma levels of creatine phosphokinase, calcium, inorganic phosphate, total bilirubin, ketones, and alkaline phosphatase did not change. Although the pancreas liver and marrow were all affected during acute toxicity, boserved changes in blood components during the chronic and terminal phases correlate best with continued hepatotoxicity. The present evidence on delayed toxicity to 4'-demethylepipodophyllotoxin 9-(4,6-o-2-thenylidene-beta-D-glucopyranoside) is most compatible with irreversible hepatotoxocity which leads to metabolic deficiencies and terminates in death of mice.
...
PMID:Acute, chronic and terminal toxicity to 4'-demethylepipodophyllotoxin thenylidene glucoside (VM26) in mice. 113 30

Cyproterone acetate (CA), an antiandrogenic compound, was used in order to investigate the role of testosterone in bone growth processes. The formation of Haversian systems in the growing antlers of white-tailed deer (Odocoileus virginianus) were substantially affected by only 3.5 mg of CA kg/wk. The mineralization processes of the bone matrix were almost completely blocked and the antlers persisted in growing throughout the whole year. Plasma levels of thyroxine, alkaline phosphatase and GH were higher while cortisol and testosterone levels were lower than in controls. No differences were registered in plasma levels of Ca, P or urea nitrogen. Despite hypertrophy of interstitial tissue, spermatogenesis was almost completely blocked. The sexual behaviour and the body appearance of experimental animals was typical for castrates.
...
PMID:The role of sex hormones in the growth of antler bone tissue. I: Endocrine and metabolic effects of antiandrogen therapy. 119 73

The operational characteristics of the Gilford System 3500 were evaluated for six months as to accuracy, precision, carry-over, reliability, and ease of operation. Accuracy was evaluated by comparison to manual methods of established accuracy. The tests evaluated and their respective correlation coefficients are as follows: glucose (0.99), blood urea nitrogen (0.99), calcium (0.97), total bilirubin (0.99), aspartate aminotransferase (0.97), alkaline phosphatase (0.98), albumin (0.96), and total protein (0.96). Within-run precision (CV) for three commercial calibration sera of differing analyte concentrations (low, intermediate, and high) were respectively: 0.69, 1.02, 1.18; 5.4, 1.2, 1.09; 0.83, 0.77, 0.86; 5.9, 1.0, 0.86; 6.4, 5.2, 2.1; 3.7, 1.5, 1.3; 0.0, 1.4, 0.97; and 1.2, 1.3, 0.75. Day-to-day precision, similarly evaluated during 101-164 days, met accepted criteria for clinically acceptable precision. Carry-over for each of the eight tests was less than 1%. Instrument reliability has been excellent, and training time is short. In summary, we have found the Gilford System 3500 to be sufficiently precise and fast, easy to operate, highly accurate, and flexible.
...
PMID:Evaluation of a discrete-sample computer-directed clinical analyzer. 124 22


<< Previous 1 2 3 4 5 6 7 8 9 10 Next >>

---