Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The concentrations of protein and copper and the activities of acid and alkaline phosphatase and beta-glucuronidase were measured in the uterine fluid of 8 25-38 year old women using the copper-T (Cu-T) 200 device for intrauterine contraception. Specimens were obtained in the proliferative phase on Cycle Days 10-12 of 1 menstrual cycle, and in the secretory phase on Days 20-23 during the next cycle prior to the insertion of the Cu-T, and during the same cycle days in Cycles 2 or 3 or 6 or 7 following insertion. Uterine fluid was obtained by irrigating the uterine cavity with physiological saline, while endometrial biopsies were taken for histological dating of the endometrium. The protein concentration of the uterine washings did not change significantly as a result of the Cu-T insertion. There was a significant difference (p.001) in Cycles 2 or 3 and 6 or 7 following insertion. Acid phosphatase activity was not influenced by the presence of the device. The betaglucuronidase in the fluid obtained during the proliferative phase showed a significant increase (p .001) DURING THE TIME WHEN the device was situ. The device caused a significant increase in the copper concentration in both phases, while the copper level in the blood serum remained unchanged. There was as increased number of white blood cells in the washings obtained during the secretory phase. The increase in the copper concentration of the uterine fluid might be the cause of the Cu-T antifertility effect due to a spermatotoxic and/or blastotoxic effect, as may the enzymic changes and increase of white blood cells.
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PMID:Intrauterine contraception with the copper-T device. 4. Influence on protein and copper concentrations and enzyme activities in uterine washings. 456 82

A patient with Wilson's disease presented at the age of 41 with a neurological defect and gross osteomalacia secondary to a defect of renal tubular reabsorption. He also showed the unusual features of a renal stone in the presence of the Fanconi syndrome and a relatively low alkaline phosphatase level, possibly due to the additional inherited defect of hypophosphatasia. During four years of treatment with penicillamine and calciferol clinical improvement was spectacular. Details of amino-acid clearances before and after treatment are given, and the results suggest that, as in the brain and the liver, the function of the distal renal tubules may be restored in Wilson's disease when copper is removed.
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PMID:Effect of treatment on renal function in severe osteomalacia due to Wilson's disease. 531 32

Rats with testicular feminization (Tfm) had been reported to have a testis specific zinc deficiency. In this report it is demonstrated that this organ specific zinc deficiency was not corrected by intraperitoneal zinc administration. Normal littermates on the other hand showed a positive testicular response to zinc administration. The increased testicular zinc level in control animals returned to normal 1 week after the zinc treatment probably due to the fast turnover of this element in the testis. Not only surgically induced cryptorchidism but also surgical cryptorchidism and epididymodeferentectomy (to simulate Tfm conditions in normal adult rats) caused a drastic reduction in testicular zinc level. Unlike in Tfm rats, however, the decrease in zinc content in operated animals was not accompanied by a corresponding decrease in alkaline phosphatase activity. Zinc concentration and alkaline phosphatase activity in plasma or other organs were not affected by the surgical procedure. The testicular copper content in the operated animals was higher than that of the unoperated controls.
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PMID:Zinc metabolism in testicular feminization and surgical cryptorchid testes in rats. 613 14

Intravenous inoculation of rabbits with spores of Absidia corymbifera strain V.73/8 produced acute phycomycosis and death within 2 to 10 days. Cultural and microscopical examination showed that fungal infection was widespread and involved most organ systems but with particularly extensive lesions developing in the kidneys. The progress of the infection was associated with a raised leucocyte count, an increasing erythrocyte sedimentation rate and significant changes in serum biochemistry. The latter included a decrease in serum iron, zinc, alkaline phosphatase and gamma-glutamyl transpeptidase concentrations but an increase in the synthesis of acute phase proteins and in the phenylalanine:tyrosine ratio and in serum concentrations of copper, magnesium, potassium, lactate dehydrogenase and triglycerides. The serum urea concentration increased substantially during the terminal phase of infection.
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PMID:Biochemical and pathological changes in experimental phycomycosis. 613 59

An histochemical method is presented to simultaneously localize, for the first time, alkaline phosphatase (ALP) and gamma-glutamyltranspeptidase (gamma-GT) in the kidney. The reaction product of ALP activity appears as a dark brown precipitate of lead sulfide, while a bright red copper chelate of an azo dye (Fast blue BBN salt) final product indicates sites of gamma-GT activity. The amalgamation of Mayahara's (ALP) and Rutenberg's (gamma-GT) techniques resulted in the demonstration of various categories of kidney tubules, according to the staining reaction of the cell brush borders: Black tubules where ALP predominates; Intermediate tubules showing a mixture of brown and red precipitates; Red tubules indicating a prevalence of gamma-GT activity; Negative tubules. A possible relation might exist between the staining characteristics observed and the different proximal tubule segments, thus allowing their distinction. In addition, this technique has the advantage to permit the concomitant study of ALP and gamma-GT distribution on the same tissue section instead of serial sections, so reducing the number of manipulations and observations as well as the amount of tissue required.
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PMID:Simultaneous visualization of alkaline phosphatase and gamma-glutamyl transpeptidase in kidney sections. 615 May 94

Ferritin binds a large quantity of Be2+ (Price D.J. and Joshi, J.G. J. Biol. Chem. 258 (1983) 10873) as well as other divalent metal ions. Therefore the ability of this protein to protect enzymes against or reverse the inhibition by metal ions was studied. Evidence presented here shows that the inhibition by Be2+ of the enzymes Na+K+ATPase, alkaline phosphatase and phosphoglucomutase is reversed by ferritin. Be2+ can be transferred reversibly between phosphoglucomutase and ferritin depending upon the relative concentrations of the 2 proteins. Ferritin also reactivated phosphoglucomutase inhibited by Zn2+, Cu2+, or Cd2+. Incubation of ferritin containing Be2+ with 4-10 fold molar excess of phosphoglucomutase (with respect to Be2+) removed 90% of the Be2+ from ferritin. The rates of inactivation of phosphoglucomutase by Be2+ donated by apoferritin or ferritin were identical. Based upon these observations it is suggested that Be2+ bound to the protein shell and to the iron core are in equilibrium with each other with the equilibrium favoring ferritin-Be2+ complex.
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PMID:Ferritin: protection of enzymatic activity against the inhibition by divalent metal ions in vitro. 633 Sep 34

Primary sclerosing cholangitis is a progressive, ultimately fatal, chronic hepatobiliary disorder for which no effective medical or surgical therapy now exists. The syndrome occurs most commonly in young men and is characterized by a chronic cholestatic syndrome, frequent association with chronic ulcerative colitis, hepatic copper overload, a paucity of serologic markers, and characteristic abnormalities in some liver-biopsy specimens and most cholangiograms. The natural history of the syndrome is unclear; the disease is likely to progress slowly and relentlessly, over a decade or longer, from an asymptomatic stage to a condition characterized by symptoms of cholestasis and complicated by cirrhosis, portal hypertension, and possibly carcinoma of the bile ducts. Screening techniques based on automated biochemical analyses are likely to lead to a diagnosis of primary sclerosing cholangitis in increasing numbers of patients, perhaps in the early, preicteric stage. An increased level of serum alkaline phosphatase in a young man, particularly if he has chronic ulcerative colitis, should strongly suggest the presence of this syndrome and the need for additional diagnostic studies. Endoscopic retrograde cholangiography and liver biopsy should be considered under these circumstances.
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PMID:Current concepts. Primary sclerosing cholangitis. 636 57

Spectrophotometric and isoelectric focusing (IEF) electrophoretic characterization of the alkaline phosphatase (ALKP) of the mosquito, Culex tarsalis, are presented. With p-nitrophenylphosphate (Pnp) as substrate, ALKP was optimally active at 37 degrees C, pH 8.0, 30 mM MgCl2, Vmax was 35.8 mumoles/10 min and the Km was 5.7 mM, with no demonstrable requirement for Zn2+. The spectrophotometric enzyme(s) was stimulated by dithiothreitol, 2-mercaptoethanol, and poly-vinylpyrollidone (PVP); inhibited by NaF, several alternative cations (Ca2+, Ba2+, Fe2+, Cu2+), and EDTA. ALKP activity was cyclic during the 15 day post-adult emergence period of the study. No significant differences were noted between the specific activities of males and females. IEF electrophoresis revealed 6 ALKP isozymes detected with alpha-naphthylphosphate within the pH range 4.0-5.5, with a second group of 3 rather indistinct species in the pH 6.0-7.0 range. IEF ALKP isozymes were stimulated by Mg2+ and PVP and inhibited by EDTA (except ALKP5.0) and cysteine; partial inhibition with phenylalanine. IEF detection of ALKP activity with Pnp indicated that the majority of the activity was localized in the pH 4.0-5.5 range, in close agreement with the alpha-naphthylphosphate results.
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PMID:Alkaline phosphatases of the mosquito, Culex tarsalis Coquillett. 646 96

Calves clinically affected with experimentally induced Johne's disease exhibited elevation of caeruloplasmin oxidase activity, and marked depression of alpha-mannosidase activity during the period when clinical signs of the disease were most prominent. Changes in serum copper levels and alkaline phosphatase activity were closely correlated with the elevation of caeruloplasmin oxidase activity, and depression of alpha-mannosidase activity. The pattern of these changes was similar to nutritional and metabolic changes described previously in acute infectious conditions in man and animals.
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PMID:Serum biochemical changes in calves with Johne's disease. 651 77

Bilaterally vasectomized langur monkeys were compared with age matched sham operated controls for biochemical constituents of the blood following 1.5, 2.0 and 2.5 years of surgery. No appreciable changes were observed in blood glucose and blood urea and serum total protein, total lipid, phospholipid, total cholesterol, sialic acid, electrolytes (chloride, phosphorus, magnesium, sodium, potassium, copper and zinc) and enzymes (LDH, alkaline phosphatase SGOT and SGPT) suggesting that long-term vasectomy does not cause adverse effects on general body metabolism including atherosclerosis.
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PMID:Effect of vasectomy on biochemical constituents of the blood in langur monkey--a 2 1/2 years follow up. 653 74


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