Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound   Target Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound

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Query: EC:3.1.3.1 (alkaline phosphatase)
47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effects of 30 days oral administration of a sublethal daily dose (0.1 g/kg body weight) of copper sulfate on the blood constituents of albino rats were studied. Percent hemoglobin, number of red blood corpuscles, plasma corpuscular volume and mean corpuscular volume were decreased significantly. No marked alteration was observed in the number of white blood corpuscles, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration and alkaline phosphatase activity. The concentrations of blood glucose, cholesterol, bilirubin and urea were elevated in the copper-fed rats. The activities of glutamic oxaloacetic transaminase, glutamic pyruvic transaminase and lactate and glutamate dehydrogenases were increased significantly, but blood total protein and acid phosphatase activity were reduced.
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PMID:Hematological indices in copper-poisoned rats. 366 Apr 31

To evaluate a critical concentration concept of cadmium (Cd) toxicity on the kidney, relationships of renal Cd level with urinary excretion of various substances--i.e., metallothionein, alkaline phosphatase, lactate dehydrogenase, N-acetyl-beta-D-glucosaminidase, total protein, Cd, copper, and zinc--were studied in Cd-injected rats. At the renal Cd concentration of 100-200 micrograms/g tissue, a dramatic increase of all these substances in urine was observed, supporting the idea of the critical concentration proposed by Friberg and co-workers (1974). The significance of increase of urinary metallothionein below this level is also discussed.
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PMID:Critical concentration of cadmium for renal toxicity in rats. 368 16

Of 187 patients with severe chronic active hepatitis, 37 (20%) had antimitochondrial antibodies, usually of low titer (less than or equal to 1:40). To assess the significance of this finding and to identify differentiating features from primary biliary cirrhosis, 24 of these patients were compared to two groups of matched counterparts of which one lacked antimitochondrial antibodies and one had the antibodies together with typical primary biliary cirrhosis. Higher serum levels of alkaline phosphatase and an increased frequency of stainable hepatic copper were the only features that distinguished these patients from those without antimitochondrial antibodies. The response to corticosteroids was not influenced by antibody status. Histologic interpretation differentiated primary biliary cirrhosis from antibody-positive chronic active hepatitis in 91% of instances. High antibody levels (greater than or equal to 1:160), immunoglobulin M concentrations (greater than or equal to 6.0 mg/ml), alkaline phosphatase activity (greater than or equal to fourfold normal), and cholesterol elevations (greater than or equal to 300 mg/dl) separated the syndromes in 82% of instances. Patients with laboratory features of primary biliary cirrhosis but histologic findings of chronic active hepatitis responded to corticosteroids. We conclude that low titers of antimitochondrial antibodies are common in chronic active hepatitis, and the presence of these antibodies does not preclude a satisfactory response to corticosteroids. Histologic features are more reliable than biochemical findings in differentiating the syndromes and should be the basis for diagnosis and treatment.
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PMID:Frequency and significance of antimitochondrial antibodies in severe chronic active hepatitis. 372 Apr 67

Low values for serum alkaline phosphatase activity were observed early in the course of two patients with Wilson's disease presenting with the combination of severe liver disease and Coombs' negative acute hemolytic anemia. A review of other cases of Wilson's disease revealed that 11 of 12 patients presenting with hemolytic anemia had values for serum alkaline phosphatase less than their respective sex- and age-adjusted mean values; in eight, serum alkaline phosphatase activity was less than the lower value for the normal range of the test. Low values for serum alkaline phosphatase were much less common in Wilson's disease patients with more chronic forms of presentation. Copper added in high concentration to serum in vitro did not have an important effect on serum alkaline phosphatase activity. The mechanism responsible for the decrease in serum alkaline phosphatase activity in patients is uncertain.
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PMID:Low serum alkaline phosphatase activity in Wilson's disease. 375 40

The effect of essential trace metals on bone metabolism was investigated in the femoral diaphysis of weanling rats. Oral administration of zinc (1.53-306 mumol/100 g body weight) for 3 days produced significant increases in alkaline phosphatase activity and DNA content. These biochemical indices were also increased by oral administration of chromium (III), cobalt, copper, manganese, and nickel with the dose of 1.53 mumol/100 g. With the dose of 15.3 mumol/100 g of above all metals, except zinc, the enzyme activity was significantly decreased in comparison with control, while DNA content was not decreased significantly. Moreover, the effect of zinc on alkaline phosphatase activity and DNA content was not enhanced by simultaneous administration of other metals (1.53 mumol/100 g). The present study indicates that, of the essential trace metals, zinc can effectively stimulate the bone growth and calcification with comparatively higher dose levels. This suggests a nutritional significance of zinc on bone growth.
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PMID:Effect of essential trace metals on bone metabolism in weanling rats: comparison with zinc and other metals' actions. 379 21

One hundred and five infants of birth weight 2000 g or less who received peripherally administered parenteral nutrition for periods of three or more weeks, were randomly assigned to groups receiving different amounts of zinc and copper supplement. The blood concentrations of zinc, copper, retinol-binding protein, prealbumin, alkaline phosphatase and aspartate transaminase were followed weekly. Mean serum zinc, retinol-binding protein and prealbumin declined significantly over time while alkaline phosphatase rose. Only the group receiving the highest zinc supplement maintained a mean serum zinc concentration within the normal range at seven weeks. No difference in the protein or enzyme concentrations was found between the different zinc supplement groups. No difference was seen in serum copper or ceruloplasmin between copper dose groups although one intravenous supplement was double that of the other.
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PMID:Serial changes in selected serum constituents in low birth weight infants on peripheral parenteral nutrition with different zinc and copper supplements. 392 51

The effects of zinc on the enzymes of femoral tissue were investigated in weanling rats that had been given zinc sulfate (1.0 mg Zn2+/100 g body wt) p.o. for 3 days. Administration of zinc caused a marked elevation of alkaline phosphatase and acid phosphatase activities, whereas it did not cause significant changes in succinate dehydrogenase, 5'-nucleotidase, ATPase, pyrophosphatase and beta-N-acetylglucosaminidase activities. The effect of zinc was greater on alkaline phosphatase of the femoral diaphysis. Zinc content of the femoral diaphysis was raised significantly by administration of zinc. The addition of zinc in concentrations of 10(-2)-10(2) microM did not produce a significant increase in alkaline phosphatase activity in the femoral diaphysis, indicating that zinc could not activate the enzyme. Administration of cycloheximide or actinomycin D completely inhibited the increase in alkaline phosphatase activity produced by administration of zinc. DNA content of the femoral diaphysis, but not epiphysis, was increased markedly by administration of zinc. The increases in both alkaline phosphatase activity and DNA content of the femoral diaphysis were not caused by administration of copper, manganese, cobalt, nickel and chromium(III). The present investigation suggests that zinc may induce the increase in alkaline phosphatase related to DNA synthesis and, as a result, stimulate bone growth.
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PMID:Action of zinc on bone metabolism in rats. Increases in alkaline phosphatase activity and DNA content. 395 86

One can find only little information about the quantitative analysis of quantity elements and trace elements in animal bone compact substance and blood serum. The authors analysed potassium, sodium, phosphorus, calcium, iron, copper, zinc, and the alkaline phosphatase in the blood serum and in the compact substance of the tibial shaft of the rabbit. The collection and the preparation of blood serum and bone for the analytic investigation and the analysis methods were shown. The concentration of the inorganic elements were determined in mol/l and the content were calculated on 1 g dry bone substance.
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PMID:[Levels of alkaline phosphatase, quantity and trace elements in the serum and compact bone in the rabbit]. 399 33

Serum biochemistry was monitored in 50 patients with otosclerosis confirmed surgically. Age distribution was 25 to 50 years (mean:30 years), and the group included 8 men. Constants determined were serum sodium, potassium, chlorine, iron, phosphorus, aluminium, magnesium, copper and alkaline phosphatase. All patients were free from systemic or metabolic disease. Results failed to demonstrate variations in sodium, potassium, iron, and chlorine concentrations when compared with controls, with non-significant differences in copper, magnesium, phosphorus, aluminium and alkaline phosphatases. These findings were observed both in young patients in whom the otosclerotic process is in the active phase, and in older patients, this excluding correlations between duration of the disease and variations in concentrations of specific ions. The presence of aluminium in the active otosclerotic process is therefore due to local factors of which the mechanism is unknown at the present time.
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PMID:[Concentration of various bioelements in the serum of otosclerosis patients]. 400 18

The two Zn(II) ions of native Escherichia coli alkaline phosphatase (EC 3.1.3.1) that are necessary for activity have been replaced by (63)Cu(II). Titration of apoenzyme with up to 2 eq of Cu(II) gives a homogeneous species with an electron spin resonance typical for Cu(II) in an axially symmetric environment, with A(z) = 496 MHz, g(z) = g = 2.27, and g(x) = g(y) = 2.05. At least seven nitrogen hyperfine lines, spaced 11 G apart, are clearly resolved on the M = +[unk] Cu(II) hyperfine peak in the parallel region. When more than 2 eq of Cu(II) are added, the electron spin resonance spectrum shows at least two types of Cu(II) binding sites; the additional site, or sites, are characterized by lower g and higher A(z) values. When Cu(II) is added to native Zn(II) alkaline phosphatase or to apoenzyme incubated with 2 eq of Zn(II), the electron spin resonance spectrum shows little or no trace of the species with higher g values and nitrogen splitting. These results indicate that the species with higher g represents copper bound at the site normally occupied by the 2 Zn (II) ions necessary for enzyme activity, and that the metal ion at this site has at least 3 equivalent nitrogen ligands, probably histidyl side chains.
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PMID:Nitrogen ligands at the active site of alkaline phosphatase. 433 43


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